Xanthomonas campestris pv. musacearum: a major constraint to banana, plantain and enset production in central and east Africa over the past decade
Taxonomy Bacteria; Phylum Proteobacteria; Class Gammaproteobacteria; Order Xanthomonadales; Family Xanthomonadaceae; Genus Xanthomonas; currently classified as X. campestris pv. musacearum (Xcm). However, fatty acid methyl ester analysis and genetic and genomic evidence suggest that this pathogen...
| Main Authors: | , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Wiley
2018
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/88052 |
| _version_ | 1855513504012828672 |
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| author | Nakato, V. Mahuku, George S. Coutinho, Teresa A. |
| author_browse | Coutinho, Teresa A. Mahuku, George S. Nakato, V. |
| author_facet | Nakato, V. Mahuku, George S. Coutinho, Teresa A. |
| author_sort | Nakato, V. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Taxonomy
Bacteria; Phylum Proteobacteria; Class Gammaproteobacteria; Order Xanthomonadales; Family Xanthomonadaceae; Genus Xanthomonas; currently classified as X. campestris pv. musacearum (Xcm). However, fatty acid methyl ester analysis and genetic and genomic evidence suggest that this pathogen is X. vasicola and resides in a separate pathovar.
Isolation and Detection
Xcm can be isolated on yeast extract peptone glucose agar (YPGA), cellobiose cephalexin agar and yeast extract tryptone sucrose agar (YTSA) complemented with 5-fluorouracil, cephalexin and cycloheximide to confer semi-selectivity. Xcm can also be identified using direct antigen coating enzyme-linked immunosorbent assay (DAC-ELISA), species-specific polymerase chain reaction (PCR) using GspDm primers and lateral flow devices that detect latent infections.
Host range
Causes Xanthomonas wilt on plants belonging to the Musaceae, primarily banana (Musa acuminata), plantain (M. acuminata × balbisiana) and enset (Ensete ventricosum).
Diversity
There is a high level of genetic homogeneity within Xcm, although genome sequencing has revealed two major sublineages.
Symptoms
Yellowing and wilting of leaves, premature fruit ripening and dry rot, bacterial exudate from cut stems.
Distribution
Xcm has only been found in African countries, namely Burundi, Ethiopia, Democratic Republic of the Congo, Kenya, Rwanda, Tanzania and Uganda.
Ecology and Epidemiology
Xcm is transmitted by insects, bats, birds and farming implements. Long-distance dispersal of the pathogen is by the transportation of latently infected plants into new areas.
Management
The management of Xcm has relied on cultural practices that keep the pathogen population at tolerable levels. Biotechnology programmes have been successful in producing resistant banana plants. However, the deployment of such genetic material has not as yet been achieved in farmers’ fields, and the sustainability of transgenic resistance remains to be addressed. |
| format | Journal Article |
| id | CGSpace88052 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace880522023-10-02T11:44:17Z Xanthomonas campestris pv. musacearum: a major constraint to banana, plantain and enset production in central and east Africa over the past decade Nakato, V. Mahuku, George S. Coutinho, Teresa A. bananas enset plantains xanthomonas genetic genome molecular biology Taxonomy Bacteria; Phylum Proteobacteria; Class Gammaproteobacteria; Order Xanthomonadales; Family Xanthomonadaceae; Genus Xanthomonas; currently classified as X. campestris pv. musacearum (Xcm). However, fatty acid methyl ester analysis and genetic and genomic evidence suggest that this pathogen is X. vasicola and resides in a separate pathovar. Isolation and Detection Xcm can be isolated on yeast extract peptone glucose agar (YPGA), cellobiose cephalexin agar and yeast extract tryptone sucrose agar (YTSA) complemented with 5-fluorouracil, cephalexin and cycloheximide to confer semi-selectivity. Xcm can also be identified using direct antigen coating enzyme-linked immunosorbent assay (DAC-ELISA), species-specific polymerase chain reaction (PCR) using GspDm primers and lateral flow devices that detect latent infections. Host range Causes Xanthomonas wilt on plants belonging to the Musaceae, primarily banana (Musa acuminata), plantain (M. acuminata × balbisiana) and enset (Ensete ventricosum). Diversity There is a high level of genetic homogeneity within Xcm, although genome sequencing has revealed two major sublineages. Symptoms Yellowing and wilting of leaves, premature fruit ripening and dry rot, bacterial exudate from cut stems. Distribution Xcm has only been found in African countries, namely Burundi, Ethiopia, Democratic Republic of the Congo, Kenya, Rwanda, Tanzania and Uganda. Ecology and Epidemiology Xcm is transmitted by insects, bats, birds and farming implements. Long-distance dispersal of the pathogen is by the transportation of latently infected plants into new areas. Management The management of Xcm has relied on cultural practices that keep the pathogen population at tolerable levels. Biotechnology programmes have been successful in producing resistant banana plants. However, the deployment of such genetic material has not as yet been achieved in farmers’ fields, and the sustainability of transgenic resistance remains to be addressed. 2018-03 2017-09-27T09:26:20Z 2017-09-27T09:26:20Z Journal Article https://hdl.handle.net/10568/88052 en Open Access Wiley Nakato, V., Mahuku, G. & Coutinho, T. (2018). Xanthomonas campestris pv. musacearum: a major constraint to banana, plantain and enset production in Central and East Africa over the past decade. Molecular Plant Pathology, 1-12. |
| spellingShingle | bananas enset plantains xanthomonas genetic genome molecular biology Nakato, V. Mahuku, George S. Coutinho, Teresa A. Xanthomonas campestris pv. musacearum: a major constraint to banana, plantain and enset production in central and east Africa over the past decade |
| title | Xanthomonas campestris pv. musacearum: a major constraint to banana, plantain and enset production in central and east Africa over the past decade |
| title_full | Xanthomonas campestris pv. musacearum: a major constraint to banana, plantain and enset production in central and east Africa over the past decade |
| title_fullStr | Xanthomonas campestris pv. musacearum: a major constraint to banana, plantain and enset production in central and east Africa over the past decade |
| title_full_unstemmed | Xanthomonas campestris pv. musacearum: a major constraint to banana, plantain and enset production in central and east Africa over the past decade |
| title_short | Xanthomonas campestris pv. musacearum: a major constraint to banana, plantain and enset production in central and east Africa over the past decade |
| title_sort | xanthomonas campestris pv musacearum a major constraint to banana plantain and enset production in central and east africa over the past decade |
| topic | bananas enset plantains xanthomonas genetic genome molecular biology |
| url | https://hdl.handle.net/10568/88052 |
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