Burkholderia pseudomallei in a lowland rice paddy: seasonal changes and influence of soil depth and physico-chemical properties
Melioidosis, a severe infection with the environmental bacterium Burkholderia pseudomallei, is being recognised increasingly frequently. What determines its uneven distribution within endemic areas is poorly understood. We cultured soil from a rice field in Laos for B. pseudomallei at different dept...
| Autores principales: | , , , , , , , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2017
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/83488 |
| _version_ | 1855522353457397760 |
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| author | Lacombe, Guillaume Ribolzi, Olivier Rochelle-Newall, E. Dance, D.A.B. Manivanh, L. Pierret, A. Rattanavong, S. Kounnavongsa, O. Buisson, Y. Elliott, I. Maeght, J.L. Xayyathip, K. Silisouk, J. Vongsouvath, M. Phetsouvanh, R. Newton, P.N. |
| author_browse | Buisson, Y. Dance, D.A.B. Elliott, I. Kounnavongsa, O. Lacombe, Guillaume Maeght, J.L. Manivanh, L. Newton, P.N. Phetsouvanh, R. Pierret, A. Rattanavong, S. Ribolzi, Olivier Rochelle-Newall, E. Silisouk, J. Vongsouvath, M. Xayyathip, K. |
| author_facet | Lacombe, Guillaume Ribolzi, Olivier Rochelle-Newall, E. Dance, D.A.B. Manivanh, L. Pierret, A. Rattanavong, S. Kounnavongsa, O. Buisson, Y. Elliott, I. Maeght, J.L. Xayyathip, K. Silisouk, J. Vongsouvath, M. Phetsouvanh, R. Newton, P.N. |
| author_sort | Lacombe, Guillaume |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Melioidosis, a severe infection with the environmental bacterium Burkholderia pseudomallei, is being recognised increasingly frequently. What determines its uneven distribution within endemic areas is poorly understood. We cultured soil from a rice field in Laos for B. pseudomallei at different depths on 4 occasions over a 13-month period. We also measured physical and chemical parameters in order to identify associated characteristics. Overall, 195 of 653 samples (29.7%) yielded B. pseudomallei. A higher prevalence of B. pseudomallei was found at soil depths greater than the 30 cm currently recommended for B. pseudomallei environmental sampling. B. pseudomallei was associated with a high soil water content and low total nitrogen, carbon and organic matter content. Our results suggested that a sampling grid of 25 five metre square quadrats (i.e. 25 × 25 m) should be sufficient to detect B. pseudomallei at a given location if samples are taken at a soil depth of at least 60 cm. However, culture of B. pseudomallei in environmental samples is difficult and liable to variation. Future studies should both rely on molecular approaches and address the micro-heterogeneity of soil when investigating physico-chemical associations with the presence of B. pseudomallei. |
| format | Journal Article |
| id | CGSpace83488 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace834882025-03-11T09:50:20Z Burkholderia pseudomallei in a lowland rice paddy: seasonal changes and influence of soil depth and physico-chemical properties Lacombe, Guillaume Ribolzi, Olivier Rochelle-Newall, E. Dance, D.A.B. Manivanh, L. Pierret, A. Rattanavong, S. Kounnavongsa, O. Buisson, Y. Elliott, I. Maeght, J.L. Xayyathip, K. Silisouk, J. Vongsouvath, M. Phetsouvanh, R. Newton, P.N. pseudomonas pseudomallei rice soil properties soil sampling soil water characteristics soil depth chemicophysical properties bacteria melioidosis Melioidosis, a severe infection with the environmental bacterium Burkholderia pseudomallei, is being recognised increasingly frequently. What determines its uneven distribution within endemic areas is poorly understood. We cultured soil from a rice field in Laos for B. pseudomallei at different depths on 4 occasions over a 13-month period. We also measured physical and chemical parameters in order to identify associated characteristics. Overall, 195 of 653 samples (29.7%) yielded B. pseudomallei. A higher prevalence of B. pseudomallei was found at soil depths greater than the 30 cm currently recommended for B. pseudomallei environmental sampling. B. pseudomallei was associated with a high soil water content and low total nitrogen, carbon and organic matter content. Our results suggested that a sampling grid of 25 five metre square quadrats (i.e. 25 × 25 m) should be sufficient to detect B. pseudomallei at a given location if samples are taken at a soil depth of at least 60 cm. However, culture of B. pseudomallei in environmental samples is difficult and liable to variation. Future studies should both rely on molecular approaches and address the micro-heterogeneity of soil when investigating physico-chemical associations with the presence of B. pseudomallei. 2017 2017-09-13T05:22:41Z 2017-09-13T05:22:41Z Journal Article https://hdl.handle.net/10568/83488 en Open Access Springer Manivanh, L.; Pierret, A.; Rattanavong, S.; Kounnavongsa, O.; Buisson, Y.; Elliott, I; Maeght, J. -L.; Xayyathip, K.; Silisouk, J.; Vongsouvath, M.; Phetsouvanh, R.; Newton, P. N.; Lacombe, Guillaume; Ribolzi, O.; Rochelle-Newall, E.; Dance, D. A. B. 2017. Burkholderia pseudomallei in a lowland rice paddy: seasonal changes and influence of soil depth and physico-chemical properties. Scientific Reports, 11p. (Online first). doi: 10.1038/s41598-017-02946-z |
| spellingShingle | pseudomonas pseudomallei rice soil properties soil sampling soil water characteristics soil depth chemicophysical properties bacteria melioidosis Lacombe, Guillaume Ribolzi, Olivier Rochelle-Newall, E. Dance, D.A.B. Manivanh, L. Pierret, A. Rattanavong, S. Kounnavongsa, O. Buisson, Y. Elliott, I. Maeght, J.L. Xayyathip, K. Silisouk, J. Vongsouvath, M. Phetsouvanh, R. Newton, P.N. Burkholderia pseudomallei in a lowland rice paddy: seasonal changes and influence of soil depth and physico-chemical properties |
| title | Burkholderia pseudomallei in a lowland rice paddy: seasonal changes and influence of soil depth and physico-chemical properties |
| title_full | Burkholderia pseudomallei in a lowland rice paddy: seasonal changes and influence of soil depth and physico-chemical properties |
| title_fullStr | Burkholderia pseudomallei in a lowland rice paddy: seasonal changes and influence of soil depth and physico-chemical properties |
| title_full_unstemmed | Burkholderia pseudomallei in a lowland rice paddy: seasonal changes and influence of soil depth and physico-chemical properties |
| title_short | Burkholderia pseudomallei in a lowland rice paddy: seasonal changes and influence of soil depth and physico-chemical properties |
| title_sort | burkholderia pseudomallei in a lowland rice paddy seasonal changes and influence of soil depth and physico chemical properties |
| topic | pseudomonas pseudomallei rice soil properties soil sampling soil water characteristics soil depth chemicophysical properties bacteria melioidosis |
| url | https://hdl.handle.net/10568/83488 |
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