Cryopreservation of Hamilin sweet orange [(Citrus sinensis (L.) Osbeck)] embryogenic calli using a modified aluminum cryo-plate technique
A modified aluminum cryo-plate technique was applied for cryopreservation of embryogenic citrus calli using aluminum cryo-plate. After encapsulation in a medium with 3% alginate, the beads were placed directly on aluminum foil troughs for exposure to plant vitrification solution (PVS2) incubated on...
| Main Authors: | , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Elsevier
2017
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/83307 |
| _version_ | 1855531972228546560 |
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| author | Duarte Souza, F.V. Kaya, E. Vieira, L.J. Silva Souza, A. Silva Carvalho, M.J. Barbosa Santos, E. Cunha Alves, A.A. Ellis, David |
| author_browse | Barbosa Santos, E. Cunha Alves, A.A. Duarte Souza, F.V. Ellis, David Kaya, E. Silva Carvalho, M.J. Silva Souza, A. Vieira, L.J. |
| author_facet | Duarte Souza, F.V. Kaya, E. Vieira, L.J. Silva Souza, A. Silva Carvalho, M.J. Barbosa Santos, E. Cunha Alves, A.A. Ellis, David |
| author_sort | Duarte Souza, F.V. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | A modified aluminum cryo-plate technique was applied for cryopreservation of embryogenic citrus calli using aluminum cryo-plate. After encapsulation in a medium with 3% alginate, the beads were placed directly on aluminum foil troughs for exposure to plant vitrification solution (PVS2) incubated on ice for three intervals (30, 45 and 60 min). After rewarming, the calli were incubated under light (16 h/8 h) or dark at 27 ± 2 °C. The survival rates ranged from 59.2% following 45 min of PVS2 exposure to 88.7% after 30 min of exposure. The 30-min PVS2 treatment yielded the highest callus growth (1.4) and percentage of calli with embryos regenerated (43.57%). The highest percentage of embryos regenerated was observed in dark condition (75.2%). The method described using aluminum foil troughs is efficient for cryopreservation of embryogenic citrus calli. |
| format | Journal Article |
| id | CGSpace83307 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace833072025-12-08T09:54:28Z Cryopreservation of Hamilin sweet orange [(Citrus sinensis (L.) Osbeck)] embryogenic calli using a modified aluminum cryo-plate technique Duarte Souza, F.V. Kaya, E. Vieira, L.J. Silva Souza, A. Silva Carvalho, M.J. Barbosa Santos, E. Cunha Alves, A.A. Ellis, David cryopreservation techniques somatic embryogenesis A modified aluminum cryo-plate technique was applied for cryopreservation of embryogenic citrus calli using aluminum cryo-plate. After encapsulation in a medium with 3% alginate, the beads were placed directly on aluminum foil troughs for exposure to plant vitrification solution (PVS2) incubated on ice for three intervals (30, 45 and 60 min). After rewarming, the calli were incubated under light (16 h/8 h) or dark at 27 ± 2 °C. The survival rates ranged from 59.2% following 45 min of PVS2 exposure to 88.7% after 30 min of exposure. The 30-min PVS2 treatment yielded the highest callus growth (1.4) and percentage of calli with embryos regenerated (43.57%). The highest percentage of embryos regenerated was observed in dark condition (75.2%). The method described using aluminum foil troughs is efficient for cryopreservation of embryogenic citrus calli. 2017-10 2017-09-01T14:25:49Z 2017-09-01T14:25:49Z Journal Article https://hdl.handle.net/10568/83307 en Limited Access Elsevier Duarte Souza, F.V.; Kaya, E.; Vieira, L.J.; Silva Souza, A.; Silva Carvalho, M.J.; Barbosa Santos, E.; Cunha Alves, A.A.; Ellis, D. 2017. Cryopreservation of Hamilin sweet orange [(Citrus sinensis (L.) Osbeck)] embryogenic calli using a modified aluminum cryo-plate technique. Scientia Horticulturae. (Netherlands). ISSN 0304-4238. 224:302-305. |
| spellingShingle | cryopreservation techniques somatic embryogenesis Duarte Souza, F.V. Kaya, E. Vieira, L.J. Silva Souza, A. Silva Carvalho, M.J. Barbosa Santos, E. Cunha Alves, A.A. Ellis, David Cryopreservation of Hamilin sweet orange [(Citrus sinensis (L.) Osbeck)] embryogenic calli using a modified aluminum cryo-plate technique |
| title | Cryopreservation of Hamilin sweet orange [(Citrus sinensis (L.) Osbeck)] embryogenic calli using a modified aluminum cryo-plate technique |
| title_full | Cryopreservation of Hamilin sweet orange [(Citrus sinensis (L.) Osbeck)] embryogenic calli using a modified aluminum cryo-plate technique |
| title_fullStr | Cryopreservation of Hamilin sweet orange [(Citrus sinensis (L.) Osbeck)] embryogenic calli using a modified aluminum cryo-plate technique |
| title_full_unstemmed | Cryopreservation of Hamilin sweet orange [(Citrus sinensis (L.) Osbeck)] embryogenic calli using a modified aluminum cryo-plate technique |
| title_short | Cryopreservation of Hamilin sweet orange [(Citrus sinensis (L.) Osbeck)] embryogenic calli using a modified aluminum cryo-plate technique |
| title_sort | cryopreservation of hamilin sweet orange citrus sinensis l osbeck embryogenic calli using a modified aluminum cryo plate technique |
| topic | cryopreservation techniques somatic embryogenesis |
| url | https://hdl.handle.net/10568/83307 |
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