Carotenoid and molecular markerbased diversity assessment in tropical yellow endosperm maize inbred lines

Maize is a staple food crop for many people in Africa where millions of children, pregnant women and nursing mothers suffer from vitamin A deficiency. The β-carotene found in yellow maize can be used as a primary source of provitamin A. Several tropical yellow endosperm maize inbred lines with varyi...

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Autores principales: Adeyemo, O. Sarah, Menkir, A., Gedil, Melaku A, Omidiji, O.
Formato: Journal Article
Lenguaje:Inglés
Publicado: 2011
Materias:
Acceso en línea:https://hdl.handle.net/10568/83278
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author Adeyemo, O. Sarah
Menkir, A.
Gedil, Melaku A
Omidiji, O.
author_browse Adeyemo, O. Sarah
Gedil, Melaku A
Menkir, A.
Omidiji, O.
author_facet Adeyemo, O. Sarah
Menkir, A.
Gedil, Melaku A
Omidiji, O.
author_sort Adeyemo, O. Sarah
collection Repository of Agricultural Research Outputs (CGSpace)
description Maize is a staple food crop for many people in Africa where millions of children, pregnant women and nursing mothers suffer from vitamin A deficiency. The β-carotene found in yellow maize can be used as a primary source of provitamin A. Several tropical yellow endosperm maize inbred lines with varying carotenoid concentrations have been identified as potential parents for breeding. Diversity assessment of these inbred lines was conducted using 17 AFLP primer combinations, 87 SSR markers and carotenoid composition to identify parents for making crosses to develop new inbred lines with high provitamin A carotenoids. Genetic distance (GD) values among all pairs of inbred lines varied from 0.08 to 0.64 with an average of 0.48 for AFLPs, and from 0.007 to 0.59 with an average of 0.45 for SSRs. The Euclidean distances calculated from carotenoid composition of the lines varied from 0.30 to 9.00 with an average of 3.27. Cluster and principal coordinate analyses based on AFLP and SSR-based GD estimates separated the lines into grouping consistent with their pedigrees. Although the correlations of molecular marker-based clustering with carotenoid-based clustering were not strong, molecular analysis could be used as the basis to identify diverse lines with complementary carotenoid compositions as parents for making bi-parental crosses to increase the level of provitamin A in tropical maize. The GD estimates can also be used as the basis to select yellow endosperm maize inbred lines for broadening and diversifying the genetic base of sources of provitamin A carotenoids in tropical yellow endosperm maize germplasm.
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spelling CGSpace832782024-01-17T12:58:34Z Carotenoid and molecular markerbased diversity assessment in tropical yellow endosperm maize inbred lines Adeyemo, O. Sarah Menkir, A. Gedil, Melaku A Omidiji, O. maize carotinoids tropical maize yellow endosperm lines ssr markers aflp markers diversity provitamin germplasm Maize is a staple food crop for many people in Africa where millions of children, pregnant women and nursing mothers suffer from vitamin A deficiency. The β-carotene found in yellow maize can be used as a primary source of provitamin A. Several tropical yellow endosperm maize inbred lines with varying carotenoid concentrations have been identified as potential parents for breeding. Diversity assessment of these inbred lines was conducted using 17 AFLP primer combinations, 87 SSR markers and carotenoid composition to identify parents for making crosses to develop new inbred lines with high provitamin A carotenoids. Genetic distance (GD) values among all pairs of inbred lines varied from 0.08 to 0.64 with an average of 0.48 for AFLPs, and from 0.007 to 0.59 with an average of 0.45 for SSRs. The Euclidean distances calculated from carotenoid composition of the lines varied from 0.30 to 9.00 with an average of 3.27. Cluster and principal coordinate analyses based on AFLP and SSR-based GD estimates separated the lines into grouping consistent with their pedigrees. Although the correlations of molecular marker-based clustering with carotenoid-based clustering were not strong, molecular analysis could be used as the basis to identify diverse lines with complementary carotenoid compositions as parents for making bi-parental crosses to increase the level of provitamin A in tropical maize. The GD estimates can also be used as the basis to select yellow endosperm maize inbred lines for broadening and diversifying the genetic base of sources of provitamin A carotenoids in tropical yellow endosperm maize germplasm. 2011-10 2017-08-29T11:07:41Z 2017-08-29T11:07:41Z Journal Article https://hdl.handle.net/10568/83278 en Limited Access Adeyemo, O., Menkir, A., Gedil, M. & Omidiji, O. (2011). Carotenoid and molecular marker-based diversity assessment in tropical yellow endosperm maize inbred lines. Journal of Food, Agriculture & Environment, 9(3&4), 383-392.
spellingShingle maize
carotinoids
tropical maize
yellow endosperm lines
ssr markers
aflp markers
diversity
provitamin
germplasm
Adeyemo, O. Sarah
Menkir, A.
Gedil, Melaku A
Omidiji, O.
Carotenoid and molecular markerbased diversity assessment in tropical yellow endosperm maize inbred lines
title Carotenoid and molecular markerbased diversity assessment in tropical yellow endosperm maize inbred lines
title_full Carotenoid and molecular markerbased diversity assessment in tropical yellow endosperm maize inbred lines
title_fullStr Carotenoid and molecular markerbased diversity assessment in tropical yellow endosperm maize inbred lines
title_full_unstemmed Carotenoid and molecular markerbased diversity assessment in tropical yellow endosperm maize inbred lines
title_short Carotenoid and molecular markerbased diversity assessment in tropical yellow endosperm maize inbred lines
title_sort carotenoid and molecular markerbased diversity assessment in tropical yellow endosperm maize inbred lines
topic maize
carotinoids
tropical maize
yellow endosperm lines
ssr markers
aflp markers
diversity
provitamin
germplasm
url https://hdl.handle.net/10568/83278
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AT menkira carotenoidandmolecularmarkerbaseddiversityassessmentintropicalyellowendospermmaizeinbredlines
AT gedilmelakua carotenoidandmolecularmarkerbaseddiversityassessmentintropicalyellowendospermmaizeinbredlines
AT omidijio carotenoidandmolecularmarkerbaseddiversityassessmentintropicalyellowendospermmaizeinbredlines