Development of a simple enzyme-linked immunosorbent assay for quantitative estimation of aflatoxin B1 albumin adduct in humans
In the present study, high titred polyclonal antibodies were produced against synthetic AFB1-lys and developed a simple indirect competitive (IC)-ELISA for quantitative estimation of AFB1-lys adducts in human serum albumin. IC-ELISA was validated by testing 250 blood samples that include 85 HBV posi...
| Autores principales: | , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
2011
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/83275 |
| _version_ | 1855536239581593600 |
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| author | Anitha, S. Waliyar, Farid Reddy, A.S. Rao, R. Kumar, P. Lava |
| author_browse | Anitha, S. Kumar, P. Lava Rao, R. Reddy, A.S. Waliyar, Farid |
| author_facet | Anitha, S. Waliyar, Farid Reddy, A.S. Rao, R. Kumar, P. Lava |
| author_sort | Anitha, S. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | In the present study, high titred polyclonal antibodies were produced against synthetic AFB1-lys and developed a simple indirect competitive (IC)-ELISA for quantitative estimation of AFB1-lys adducts in human serum albumin. IC-ELISA was validated by testing 250 blood samples that include 85 HBV positive samples from unidentified subjects from the Apollo Health City, Hyderabad, and 165 blood samples collected from ICRISAT campus, Hyderabad, India. All the samples that were positive to AFB1-lys were from HBV-positive subjects, indicating a potential risk of hepatocellular carcinoma in 4.8% of the subjects tested in the present study. This validation confirms the suitability of IC-ELISA, which is simple, cost-effective and enables high-throughput analysis. |
| format | Journal Article |
| id | CGSpace83275 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2011 |
| publishDateRange | 2011 |
| publishDateSort | 2011 |
| record_format | dspace |
| spelling | CGSpace832752024-01-17T12:58:34Z Development of a simple enzyme-linked immunosorbent assay for quantitative estimation of aflatoxin B1 albumin adduct in humans Anitha, S. Waliyar, Farid Reddy, A.S. Rao, R. Kumar, P. Lava aflatoxins toxin contamination fungal infestation aflatoxin exposure In the present study, high titred polyclonal antibodies were produced against synthetic AFB1-lys and developed a simple indirect competitive (IC)-ELISA for quantitative estimation of AFB1-lys adducts in human serum albumin. IC-ELISA was validated by testing 250 blood samples that include 85 HBV positive samples from unidentified subjects from the Apollo Health City, Hyderabad, and 165 blood samples collected from ICRISAT campus, Hyderabad, India. All the samples that were positive to AFB1-lys were from HBV-positive subjects, indicating a potential risk of hepatocellular carcinoma in 4.8% of the subjects tested in the present study. This validation confirms the suitability of IC-ELISA, which is simple, cost-effective and enables high-throughput analysis. 2011-10-10 2017-08-28T14:03:31Z 2017-08-28T14:03:31Z Journal Article https://hdl.handle.net/10568/83275 en Limited Access Anitha, S., Waliyar, F., Reddy, A.S., Rao, R., Rao, R. & Kumar, P.L. (2011). Development of a simple enzyme-linked immunosorbent assay for quantitative estimation of aflatoxin B1 albumin adduct in humans. Current Science, 101(7), 844-846. |
| spellingShingle | aflatoxins toxin contamination fungal infestation aflatoxin exposure Anitha, S. Waliyar, Farid Reddy, A.S. Rao, R. Kumar, P. Lava Development of a simple enzyme-linked immunosorbent assay for quantitative estimation of aflatoxin B1 albumin adduct in humans |
| title | Development of a simple enzyme-linked immunosorbent assay for quantitative estimation of aflatoxin B1 albumin adduct in humans |
| title_full | Development of a simple enzyme-linked immunosorbent assay for quantitative estimation of aflatoxin B1 albumin adduct in humans |
| title_fullStr | Development of a simple enzyme-linked immunosorbent assay for quantitative estimation of aflatoxin B1 albumin adduct in humans |
| title_full_unstemmed | Development of a simple enzyme-linked immunosorbent assay for quantitative estimation of aflatoxin B1 albumin adduct in humans |
| title_short | Development of a simple enzyme-linked immunosorbent assay for quantitative estimation of aflatoxin B1 albumin adduct in humans |
| title_sort | development of a simple enzyme linked immunosorbent assay for quantitative estimation of aflatoxin b1 albumin adduct in humans |
| topic | aflatoxins toxin contamination fungal infestation aflatoxin exposure |
| url | https://hdl.handle.net/10568/83275 |
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