Cryopreservation of cassava zygotic embryos and whole seeds in liquid nitrogen
Cryopreservation in liquid nitrogen of isolated zygotic embryos and whole seeds of cassava have been studied. A simple method is proposed for utilization in germplasm conservation or other related uses. Very high percentage survival values (ca. 97 percent) of zygotic embryos and whole seeds were obt...
| Autores principales: | , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
1990
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/83253 |
| _version_ | 1855520739305717760 |
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| author | Marín, M.L. Mafla, G. Roca, W. Withers, Lyndsey A. |
| author_browse | Mafla, G. Marín, M.L. Roca, W. Withers, Lyndsey A. |
| author_facet | Marín, M.L. Mafla, G. Roca, W. Withers, Lyndsey A. |
| author_sort | Marín, M.L. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Cryopreservation in liquid nitrogen of isolated zygotic embryos and whole seeds of cassava have been studied. A simple method is proposed for utilization in germplasm conservation or other related uses. Very high percentage survival values (ca. 97 percent) of zygotic embryos and whole seeds were obtained by both slow and rapid cooling methods followed by slow thawing (1 hour at -70 degrees C followed by 1 hour at -15 degrees C). In the case of whole seeds, slow thawing prevented shattering. Recovery of whole plants occurred from all surviving seeds but was reduced to 25-34 percent for control and frozen embryos. This drop is attributed to physical injury during dissection. (AS) |
| format | Journal Article |
| id | CGSpace83253 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1990 |
| publishDateRange | 1990 |
| publishDateSort | 1990 |
| record_format | dspace |
| spelling | CGSpace832532024-03-06T10:16:43Z Cryopreservation of cassava zygotic embryos and whole seeds in liquid nitrogen Marín, M.L. Mafla, G. Roca, W. Withers, Lyndsey A. manihot esculenta cryopreservation biotechnology seed temperature germination water content storage criopreservacion biotecnología semillas temperatura germinacion contenido de agua almacenamiento Cryopreservation in liquid nitrogen of isolated zygotic embryos and whole seeds of cassava have been studied. A simple method is proposed for utilization in germplasm conservation or other related uses. Very high percentage survival values (ca. 97 percent) of zygotic embryos and whole seeds were obtained by both slow and rapid cooling methods followed by slow thawing (1 hour at -70 degrees C followed by 1 hour at -15 degrees C). In the case of whole seeds, slow thawing prevented shattering. Recovery of whole plants occurred from all surviving seeds but was reduced to 25-34 percent for control and frozen embryos. This drop is attributed to physical injury during dissection. (AS) 1990 2017-08-28T12:45:48Z 2017-08-28T12:45:48Z Journal Article https://hdl.handle.net/10568/83253 en Limited Access Marin, M.L.; Mafla, G.; Roca, W.M.; Withers, L.A. 1990. Cryopreservation of cassava zygotic embryos and whole seeds in liquid nitrogen. Cryo-Letters 11:257-264. |
| spellingShingle | manihot esculenta cryopreservation biotechnology seed temperature germination water content storage criopreservacion biotecnología semillas temperatura germinacion contenido de agua almacenamiento Marín, M.L. Mafla, G. Roca, W. Withers, Lyndsey A. Cryopreservation of cassava zygotic embryos and whole seeds in liquid nitrogen |
| title | Cryopreservation of cassava zygotic embryos and whole seeds in liquid nitrogen |
| title_full | Cryopreservation of cassava zygotic embryos and whole seeds in liquid nitrogen |
| title_fullStr | Cryopreservation of cassava zygotic embryos and whole seeds in liquid nitrogen |
| title_full_unstemmed | Cryopreservation of cassava zygotic embryos and whole seeds in liquid nitrogen |
| title_short | Cryopreservation of cassava zygotic embryos and whole seeds in liquid nitrogen |
| title_sort | cryopreservation of cassava zygotic embryos and whole seeds in liquid nitrogen |
| topic | manihot esculenta cryopreservation biotechnology seed temperature germination water content storage criopreservacion biotecnología semillas temperatura germinacion contenido de agua almacenamiento |
| url | https://hdl.handle.net/10568/83253 |
| work_keys_str_mv | AT marinml cryopreservationofcassavazygoticembryosandwholeseedsinliquidnitrogen AT maflag cryopreservationofcassavazygoticembryosandwholeseedsinliquidnitrogen AT rocaw cryopreservationofcassavazygoticembryosandwholeseedsinliquidnitrogen AT witherslyndseya cryopreservationofcassavazygoticembryosandwholeseedsinliquidnitrogen |