In vitro methods of germ-plasm conservation
For many crops with preferential vegetative propagation, or which are sterile, or have seed unresponsive to standard seed storage techniques, in vitro methods can provide a valuable adjunct to other germplasm conservation strategies. Two types of in vitro gene banks are proposed: (i) an in vitro act...
| Autores principales: | , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Canadian Science Publishing
1989
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/83252 |
| _version_ | 1855532896227426304 |
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| author | Roca, W. Chavez, R. Martin, M.L. Arias, D.I. Mafla, G. Reyes, Raúl |
| author_browse | Arias, D.I. Chavez, R. Mafla, G. Martin, M.L. Reyes, Raúl Roca, W. |
| author_facet | Roca, W. Chavez, R. Martin, M.L. Arias, D.I. Mafla, G. Reyes, Raúl |
| author_sort | Roca, W. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | For many crops with preferential vegetative propagation, or which are sterile, or have seed unresponsive to standard seed storage techniques, in vitro methods can provide a valuable adjunct to other germplasm conservation strategies. Two types of in vitro gene banks are proposed: (i) an in vitro active gene bank where cultures are maintained under slow growth; of the few of these that exist, the cassava (Manihot) in vitro active gene bank of the Centro Internacional de Agricultura Tropical (CIAT) consists of over 4000 clones; (ii) an in vitro base gene bank, where cultures are cryopreserved; at present, none of this type exist. In vitro conservation offers a means of maintaining valuable gene combinations in a small space, protected against pest and disease attack, soil problems, and climatic changes, and with high multiplication potential. The main limitations are inability to regenerate plants of many important crops and the risk of genetic instability of cultures. A joint project of the International Board for Plant Genetic Resources and CIAT is underway to develop an in vitro based set of procedures for handling germplasm, using cassava as a model, with culture maintenance, stability monitoring, and management data bases being considered. Results of recent work on in vitro conservation and related activities, i.e., germ-plasm collection, exchange, and characterization, will be presented and discussed, with special reference to Manihot. (AS) |
| format | Journal Article |
| id | CGSpace83252 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1989 |
| publishDateRange | 1989 |
| publishDateSort | 1989 |
| publisher | Canadian Science Publishing |
| publisherStr | Canadian Science Publishing |
| record_format | dspace |
| spelling | CGSpace832522024-01-17T12:58:34Z In vitro methods of germ-plasm conservation Roca, W. Chavez, R. Martin, M.L. Arias, D.I. Mafla, G. Reyes, Raúl manihot esculenta gene banks tissue culture cryopreservation electrophoresis genetic variation starch crops bancos de genes cultivo de tejidos criopreservacion electroforesis variacion genetica For many crops with preferential vegetative propagation, or which are sterile, or have seed unresponsive to standard seed storage techniques, in vitro methods can provide a valuable adjunct to other germplasm conservation strategies. Two types of in vitro gene banks are proposed: (i) an in vitro active gene bank where cultures are maintained under slow growth; of the few of these that exist, the cassava (Manihot) in vitro active gene bank of the Centro Internacional de Agricultura Tropical (CIAT) consists of over 4000 clones; (ii) an in vitro base gene bank, where cultures are cryopreserved; at present, none of this type exist. In vitro conservation offers a means of maintaining valuable gene combinations in a small space, protected against pest and disease attack, soil problems, and climatic changes, and with high multiplication potential. The main limitations are inability to regenerate plants of many important crops and the risk of genetic instability of cultures. A joint project of the International Board for Plant Genetic Resources and CIAT is underway to develop an in vitro based set of procedures for handling germplasm, using cassava as a model, with culture maintenance, stability monitoring, and management data bases being considered. Results of recent work on in vitro conservation and related activities, i.e., germ-plasm collection, exchange, and characterization, will be presented and discussed, with special reference to Manihot. (AS) 1989-01-15 2017-08-28T12:45:48Z 2017-08-28T12:45:48Z Journal Article https://hdl.handle.net/10568/83252 en Limited Access Canadian Science Publishing Roca, William M.; Chavez, R.; Martin, M.L.; Arias, D.I.; Mafla, G.; Reyes, Raúl. 1989. In vitro methods of germplasm conservation. Genome 31(2):813-817. |
| spellingShingle | manihot esculenta gene banks tissue culture cryopreservation electrophoresis genetic variation starch crops bancos de genes cultivo de tejidos criopreservacion electroforesis variacion genetica Roca, W. Chavez, R. Martin, M.L. Arias, D.I. Mafla, G. Reyes, Raúl In vitro methods of germ-plasm conservation |
| title | In vitro methods of germ-plasm conservation |
| title_full | In vitro methods of germ-plasm conservation |
| title_fullStr | In vitro methods of germ-plasm conservation |
| title_full_unstemmed | In vitro methods of germ-plasm conservation |
| title_short | In vitro methods of germ-plasm conservation |
| title_sort | in vitro methods of germ plasm conservation |
| topic | manihot esculenta gene banks tissue culture cryopreservation electrophoresis genetic variation starch crops bancos de genes cultivo de tejidos criopreservacion electroforesis variacion genetica |
| url | https://hdl.handle.net/10568/83252 |
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