An energy-dispersive X-ray fluorescence method for analysing Fe and Zn in common bean, maize and cowpea biofortification programs
Background and aims Biofortification breeding programs have the need for rapid and accurate screening methods to identify nutrient dense genotypes. This study explores the use of energy dispersive x-ray fluorescence (EDXRF) for the rapid screening of iron (Fe) and zinc (Zn) concentration in three c...
| Main Authors: | , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Springer
2017
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/83153 |
| _version_ | 1855525235882721280 |
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| author | Guild, Georgia E. Paltridge, Nicholas G. Andersson, Meike S. Stangoulis, James C.R. |
| author_browse | Andersson, Meike S. Guild, Georgia E. Paltridge, Nicholas G. Stangoulis, James C.R. |
| author_facet | Guild, Georgia E. Paltridge, Nicholas G. Andersson, Meike S. Stangoulis, James C.R. |
| author_sort | Guild, Georgia E. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Background and aims
Biofortification breeding programs have the need for rapid and accurate screening methods to identify nutrient dense genotypes. This study explores the use of energy dispersive x-ray fluorescence (EDXRF) for the rapid screening of iron (Fe) and zinc (Zn) concentration in three coarse-grain crops; common bean, maize and cowpea.
Methods
Bean, maize and cowpea seed was provided from biofortification breeding programs and analysed with Inductively Coupled Plasma - Mass Spectrometry (ICP-MS) to determine reference Fe and Zn concentrations. A subset of samples for each crop was used to calibrate for Zn and Fe and a separate set of samples used to validate the XRF method.
Results
Results indicate that when analysing bean, maize and cowpea flour samples, the EDXRF results were not significantly different to the reference ICP-MS analysis, with an average difference of ± 1 mg kg−1 for both Fe and Zn.
Conclusion
EDXRF analysis of common beans, cowpea and maize flour enables rapid and accurate analysis when screening for Fe and Zn in bean, maize and cowpea. |
| format | Journal Article |
| id | CGSpace83153 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace831532025-03-13T09:44:16Z An energy-dispersive X-ray fluorescence method for analysing Fe and Zn in common bean, maize and cowpea biofortification programs Guild, Georgia E. Paltridge, Nicholas G. Andersson, Meike S. Stangoulis, James C.R. food enrichment food fortification biofortied crops micronutrients breeding plant enriquecimiento de los alimentos fortificación de alimentos micronutrientes mejora Background and aims Biofortification breeding programs have the need for rapid and accurate screening methods to identify nutrient dense genotypes. This study explores the use of energy dispersive x-ray fluorescence (EDXRF) for the rapid screening of iron (Fe) and zinc (Zn) concentration in three coarse-grain crops; common bean, maize and cowpea. Methods Bean, maize and cowpea seed was provided from biofortification breeding programs and analysed with Inductively Coupled Plasma - Mass Spectrometry (ICP-MS) to determine reference Fe and Zn concentrations. A subset of samples for each crop was used to calibrate for Zn and Fe and a separate set of samples used to validate the XRF method. Results Results indicate that when analysing bean, maize and cowpea flour samples, the EDXRF results were not significantly different to the reference ICP-MS analysis, with an average difference of ± 1 mg kg−1 for both Fe and Zn. Conclusion EDXRF analysis of common beans, cowpea and maize flour enables rapid and accurate analysis when screening for Fe and Zn in bean, maize and cowpea. 2017-10 2017-08-15T18:39:09Z 2017-08-15T18:39:09Z Journal Article https://hdl.handle.net/10568/83153 en Open Access Springer Guild, Georgia E.; Paltridge, Nicholas G.; Andersson, Meike S.; Stangoulis, James C. R.. 2017. An energy-dispersive X-ray fluorescence method for analysing Fe and Zn in common bean, maize and cowpea biofortification programs . Plant and Soil 419(1-2): 457-466. |
| spellingShingle | food enrichment food fortification biofortied crops micronutrients breeding plant enriquecimiento de los alimentos fortificación de alimentos micronutrientes mejora Guild, Georgia E. Paltridge, Nicholas G. Andersson, Meike S. Stangoulis, James C.R. An energy-dispersive X-ray fluorescence method for analysing Fe and Zn in common bean, maize and cowpea biofortification programs |
| title | An energy-dispersive X-ray fluorescence method for analysing Fe and Zn in common bean, maize and cowpea biofortification programs |
| title_full | An energy-dispersive X-ray fluorescence method for analysing Fe and Zn in common bean, maize and cowpea biofortification programs |
| title_fullStr | An energy-dispersive X-ray fluorescence method for analysing Fe and Zn in common bean, maize and cowpea biofortification programs |
| title_full_unstemmed | An energy-dispersive X-ray fluorescence method for analysing Fe and Zn in common bean, maize and cowpea biofortification programs |
| title_short | An energy-dispersive X-ray fluorescence method for analysing Fe and Zn in common bean, maize and cowpea biofortification programs |
| title_sort | energy dispersive x ray fluorescence method for analysing fe and zn in common bean maize and cowpea biofortification programs |
| topic | food enrichment food fortification biofortied crops micronutrients breeding plant enriquecimiento de los alimentos fortificación de alimentos micronutrientes mejora |
| url | https://hdl.handle.net/10568/83153 |
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