Screening for potential Striga hermonthica fungal and bacterial biocontrol agents from suppressive soils in Western Kenya
Striga hermonthica is a hemiparasitic weed that causes huge grain yield losses to small-scale farmers in Africa. Effective biocontrol agents against S. hermonthica can sustainably mitigate these losses. This study characterized the biocontrol potential of culturable fungal and bacterial isolates fro...
| Main Authors: | , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
2017
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/82971 |
| _version_ | 1855542024395030528 |
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| author | Neondo, J.O. Alakonya, A.E. Kasili, R.W. |
| author_browse | Alakonya, A.E. Kasili, R.W. Neondo, J.O. |
| author_facet | Neondo, J.O. Alakonya, A.E. Kasili, R.W. |
| author_sort | Neondo, J.O. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Striga hermonthica is a hemiparasitic weed that causes huge grain yield losses to small-scale farmers in Africa. Effective biocontrol agents against S. hermonthica can sustainably mitigate these losses. This study characterized the biocontrol potential of culturable fungal and bacterial isolates from S. hermonthica suppressive soils of western Kenya. These isolates were screened for their ability to produce antibiotic compounds and extra cellular enzymes and also their ability to cause S. hermonthica seed decay. Genomic DNA of the selected bacterial and fungal isolates was extracted and partial characterization of 16S rRNA and 18S rRNA genes performed respectively. Analysis show that antibiosis and enzymatic properties of potential biocontrol isolates correlated positively. Isolate KY041696 recorded high antibiosis, enzymatic and seed decay values. This study also revealed that bioactive bacterial isolates belonged to Bacillus, Streptomyces and Rhizobium genera. In this study, no fungal isolate caused S. hermonthica seed decay. This study therefore provides baseline information on the potential biocontrol microbes against S. hermonthica in Western Kenya that could be exploited further in the management of the weed. |
| format | Journal Article |
| id | CGSpace82971 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | CGSpace829712023-12-08T19:43:41Z Screening for potential Striga hermonthica fungal and bacterial biocontrol agents from suppressive soils in Western Kenya Neondo, J.O. Alakonya, A.E. Kasili, R.W. exudate antibiosis dna biocontrol agent extra-cellular enzymes striga hermonthica fungal western kenya Striga hermonthica is a hemiparasitic weed that causes huge grain yield losses to small-scale farmers in Africa. Effective biocontrol agents against S. hermonthica can sustainably mitigate these losses. This study characterized the biocontrol potential of culturable fungal and bacterial isolates from S. hermonthica suppressive soils of western Kenya. These isolates were screened for their ability to produce antibiotic compounds and extra cellular enzymes and also their ability to cause S. hermonthica seed decay. Genomic DNA of the selected bacterial and fungal isolates was extracted and partial characterization of 16S rRNA and 18S rRNA genes performed respectively. Analysis show that antibiosis and enzymatic properties of potential biocontrol isolates correlated positively. Isolate KY041696 recorded high antibiosis, enzymatic and seed decay values. This study also revealed that bioactive bacterial isolates belonged to Bacillus, Streptomyces and Rhizobium genera. In this study, no fungal isolate caused S. hermonthica seed decay. This study therefore provides baseline information on the potential biocontrol microbes against S. hermonthica in Western Kenya that could be exploited further in the management of the weed. 2017-07-18 2017-08-01T10:12:33Z 2017-08-01T10:12:33Z Journal Article https://hdl.handle.net/10568/82971 en Limited Access Neondo, J.O., Alakonya, A.E. & Kasili, R.W. (2017). Screening for potential Striga hermonthica fungal and bacterial biocontrol agents from suppressive soils in Western Kenya. BioControl, 1-13. |
| spellingShingle | exudate antibiosis dna biocontrol agent extra-cellular enzymes striga hermonthica fungal western kenya Neondo, J.O. Alakonya, A.E. Kasili, R.W. Screening for potential Striga hermonthica fungal and bacterial biocontrol agents from suppressive soils in Western Kenya |
| title | Screening for potential Striga hermonthica fungal and bacterial biocontrol agents from suppressive soils in Western Kenya |
| title_full | Screening for potential Striga hermonthica fungal and bacterial biocontrol agents from suppressive soils in Western Kenya |
| title_fullStr | Screening for potential Striga hermonthica fungal and bacterial biocontrol agents from suppressive soils in Western Kenya |
| title_full_unstemmed | Screening for potential Striga hermonthica fungal and bacterial biocontrol agents from suppressive soils in Western Kenya |
| title_short | Screening for potential Striga hermonthica fungal and bacterial biocontrol agents from suppressive soils in Western Kenya |
| title_sort | screening for potential striga hermonthica fungal and bacterial biocontrol agents from suppressive soils in western kenya |
| topic | exudate antibiosis dna biocontrol agent extra-cellular enzymes striga hermonthica fungal western kenya |
| url | https://hdl.handle.net/10568/82971 |
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