Development of ELISA for the detection of Xanthomonas campestris pv. musacearum, the causal agent of BXW: Banana Xanthomonas Wilt
Rabbit polyclonal antibodies (PAbs) were produced against a pure isolate of Xanthomonas campestris pv. musacearum (Xcm), which is the causal organism of banana Xanthomonas wilt (BXW) disease of banana (Musa spp.) in the Great Lakes region of Africa. Anti-Xcm PAbs were used to develop a direct antige...
| Main Authors: | , , , , |
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| Format: | Book Chapter |
| Language: | Inglés |
| Published: |
CAB International
2013
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/82866 |
| _version_ | 1855542550462464000 |
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| author | Nakato, G.V. Akinbade, S.A. Kumar, P. Lava Bandyopadhyay, Ranajit Beed, Fenton D. |
| author_browse | Akinbade, S.A. Bandyopadhyay, Ranajit Beed, Fenton D. Kumar, P. Lava Nakato, G.V. |
| author_facet | Nakato, G.V. Akinbade, S.A. Kumar, P. Lava Bandyopadhyay, Ranajit Beed, Fenton D. |
| author_sort | Nakato, G.V. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Rabbit polyclonal antibodies (PAbs) were produced against a pure isolate of Xanthomonas campestris pv. musacearum (Xcm), which is the causal organism of banana Xanthomonas wilt (BXW) disease of banana (Musa spp.) in the Great Lakes region of Africa. Anti-Xcm PAbs were used to develop a direct antigen coating enzyme-linked immunosorbent assay (DAC-ELISA) for the detection of Xcm in leaf and pseudostem tissues of infected banana and plantain (Musa spp.). The anti-Xcm PAbs in DAC-ELISA specifically reacted with Xcm but not with Escherichia coli, X. vasicola pv. vasculorum of maize and sugarcane or X. vasicola pv.
holcicola of sorghum. Results of the DAC-ELISA detection of Xcm are comparable with the results of PCR and culture-based detection assays for the same pathogen. |
| format | Book Chapter |
| id | CGSpace82866 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2013 |
| publishDateRange | 2013 |
| publishDateSort | 2013 |
| publisher | CAB International |
| publisherStr | CAB International |
| record_format | dspace |
| spelling | CGSpace828662023-02-15T06:34:05Z Development of ELISA for the detection of Xanthomonas campestris pv. musacearum, the causal agent of BXW: Banana Xanthomonas Wilt Nakato, G.V. Akinbade, S.A. Kumar, P. Lava Bandyopadhyay, Ranajit Beed, Fenton D. polyclonal antibodies bananas diseases rabbit polyclonal antibodies banana xanthomonas wilt xanthomonas campestris pv. musacearum Rabbit polyclonal antibodies (PAbs) were produced against a pure isolate of Xanthomonas campestris pv. musacearum (Xcm), which is the causal organism of banana Xanthomonas wilt (BXW) disease of banana (Musa spp.) in the Great Lakes region of Africa. Anti-Xcm PAbs were used to develop a direct antigen coating enzyme-linked immunosorbent assay (DAC-ELISA) for the detection of Xcm in leaf and pseudostem tissues of infected banana and plantain (Musa spp.). The anti-Xcm PAbs in DAC-ELISA specifically reacted with Xcm but not with Escherichia coli, X. vasicola pv. vasculorum of maize and sugarcane or X. vasicola pv. holcicola of sorghum. Results of the DAC-ELISA detection of Xcm are comparable with the results of PCR and culture-based detection assays for the same pathogen. 2013-12 2017-07-25T10:26:01Z 2017-07-25T10:26:01Z Book Chapter https://hdl.handle.net/10568/82866 en Limited Access CAB International Nakato, G.V., Akinbada, S.A., Kumar, P.L., Bandyopadhyay, R. & Beed, F. (2013). Development of ELISA for the detection of Xanthomonas campestris pv. musacearum, the causal agent of BXW: Banana Xanthomonas Wilt. In G. Blomme, P. van Asten and B. Vanlauwe, Banana systems in the humid highlands of sub-Saharan Africa. (p. 93-100). Wallingford: CABI. |
| spellingShingle | polyclonal antibodies bananas diseases rabbit polyclonal antibodies banana xanthomonas wilt xanthomonas campestris pv. musacearum Nakato, G.V. Akinbade, S.A. Kumar, P. Lava Bandyopadhyay, Ranajit Beed, Fenton D. Development of ELISA for the detection of Xanthomonas campestris pv. musacearum, the causal agent of BXW: Banana Xanthomonas Wilt |
| title | Development of ELISA for the detection of Xanthomonas campestris pv. musacearum, the causal agent of BXW: Banana Xanthomonas Wilt |
| title_full | Development of ELISA for the detection of Xanthomonas campestris pv. musacearum, the causal agent of BXW: Banana Xanthomonas Wilt |
| title_fullStr | Development of ELISA for the detection of Xanthomonas campestris pv. musacearum, the causal agent of BXW: Banana Xanthomonas Wilt |
| title_full_unstemmed | Development of ELISA for the detection of Xanthomonas campestris pv. musacearum, the causal agent of BXW: Banana Xanthomonas Wilt |
| title_short | Development of ELISA for the detection of Xanthomonas campestris pv. musacearum, the causal agent of BXW: Banana Xanthomonas Wilt |
| title_sort | development of elisa for the detection of xanthomonas campestris pv musacearum the causal agent of bxw banana xanthomonas wilt |
| topic | polyclonal antibodies bananas diseases rabbit polyclonal antibodies banana xanthomonas wilt xanthomonas campestris pv. musacearum |
| url | https://hdl.handle.net/10568/82866 |
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