Organogenesis in-vitro using three tissues types of tree tomato [Solanum betaceum (Cav.)]
Tree tomato is a fruit with great economic potential due to its high nutritional value. The induction of direct organogenesis in this species is a great alternative for clonal propagation of disease-free plants, and also useful for genetic transformation. In this study was assessed the induction of...
| Main Authors: | , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Universidad Nacional de Colombia
2017
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/82859 |
| _version_ | 1855517681975820288 |
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| author | Murillo-Gómez, Paola Andrea Hoyos S, Rodrigo Chavarriaga Aguirre, Paul |
| author_browse | Chavarriaga Aguirre, Paul Hoyos S, Rodrigo Murillo-Gómez, Paola Andrea |
| author_facet | Murillo-Gómez, Paola Andrea Hoyos S, Rodrigo Chavarriaga Aguirre, Paul |
| author_sort | Murillo-Gómez, Paola Andrea |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Tree tomato is a fruit with great economic potential due to its high nutritional value. The induction of direct organogenesis in this species is a great alternative for clonal propagation of disease-free plants, and also useful for genetic transformation. In this study was assessed the induction of organogenesis in vitro using three different tissues: leaves, petioles and sexual seeds of the variety Common. All tissues were cultured on MS supplemented with agar, sucrose and TDZ or BAP phytohormones at 0.5 to 3 mg L-1, combined or not with the auxins IAA and NAA. Although petioles and seeds had a great potential for regeneration of plantlets, leaves produced more shoots (average of 18.4 shoots/explant) on medium containing TDZ 0.5 mg L-1. Additionally, its easy handling and resistance to physical damage may be useful traits to perform genetic transformation. The highest average of shoots produced from seeds and petioles were 4.3 and 3.1 shoots/explant, respectively. The production of multiple shoots in petioles was influenced by low concentrations of auxins. The emergence of the radicle in the seeds was important for the formation of shoots. |
| format | Journal Article |
| id | CGSpace82859 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| publisher | Universidad Nacional de Colombia |
| publisherStr | Universidad Nacional de Colombia |
| record_format | dspace |
| spelling | CGSpace828592025-03-13T09:44:01Z Organogenesis in-vitro using three tissues types of tree tomato [Solanum betaceum (Cav.)] Organogénesis in-vitro usando tres tipos de tejidos de tomate de árbol [ Solanum betaceum (Cav.)] Murillo-Gómez, Paola Andrea Hoyos S, Rodrigo Chavarriaga Aguirre, Paul biotechnology propagation tissue culture plant growth substances biotecnología propagación cultivo de tejidos sustancias de crecimiento vegetal Tree tomato is a fruit with great economic potential due to its high nutritional value. The induction of direct organogenesis in this species is a great alternative for clonal propagation of disease-free plants, and also useful for genetic transformation. In this study was assessed the induction of organogenesis in vitro using three different tissues: leaves, petioles and sexual seeds of the variety Common. All tissues were cultured on MS supplemented with agar, sucrose and TDZ or BAP phytohormones at 0.5 to 3 mg L-1, combined or not with the auxins IAA and NAA. Although petioles and seeds had a great potential for regeneration of plantlets, leaves produced more shoots (average of 18.4 shoots/explant) on medium containing TDZ 0.5 mg L-1. Additionally, its easy handling and resistance to physical damage may be useful traits to perform genetic transformation. The highest average of shoots produced from seeds and petioles were 4.3 and 3.1 shoots/explant, respectively. The production of multiple shoots in petioles was influenced by low concentrations of auxins. The emergence of the radicle in the seeds was important for the formation of shoots. El tomate de árbol es un frutal con gran potencial económico debido a su alto valor nutricional. La inducción de organogénesis directa en esta especie presenta una gran alternativa para la propagación clonal de plantas libres de enfermedades, y también es útil para transformación genética. En este estudio fue evaluada inducción de organogénesis in vitro utilizando tres diferentes tejidos: hojas, pecíolos y semillas sexuales, de la variedad Común. Todos los tejidos se cultivaron en MS suplementado con agar, sacarosa y las fitohormonas TDZ o BAP de 0.5 a 3 mg L-1, combinadas o no con las auxinas AIA y ANA. Aunque los pecíolos y las semillas tuvieron un gran potencial para la regeneración de plántulas, las hojas produjeron más número de brotes (promedio de 18.4 brotes/explante) en medio que contenía TDZ 0.5 mg L-1. Además, su fácil manejo y resistencia al daño físico pueden ser rasgos útiles para realizar transformación genética. El promedio más alto de brotes producidos a partir de semillas y pecíolos fue de 4.3 y 3.1 brotes/ explante, respectivamente. La producción de brotes múltiples en pecíolos fue influenciada por bajas concentraciones de auxinas. La emergencia de la radícula en las semillas fue importante en la formación de brotes. 2017 2017-07-24T18:37:16Z 2017-07-24T18:37:16Z Journal Article https://hdl.handle.net/10568/82859 en Open Access Universidad Nacional de Colombia Murillo-Gómez, Paola Andrea; Hoyos S., Rodrigo; Chavarriaga, Paul. 2017. Organogenesis in-vitro using three tissues types of tree tomato [Solanum betaceum (Cav.)]. Agronomía Colombiana 35(1): 5-11 |
| spellingShingle | biotechnology propagation tissue culture plant growth substances biotecnología propagación cultivo de tejidos sustancias de crecimiento vegetal Murillo-Gómez, Paola Andrea Hoyos S, Rodrigo Chavarriaga Aguirre, Paul Organogenesis in-vitro using three tissues types of tree tomato [Solanum betaceum (Cav.)] |
| title | Organogenesis in-vitro using three tissues types of tree tomato [Solanum betaceum (Cav.)] |
| title_full | Organogenesis in-vitro using three tissues types of tree tomato [Solanum betaceum (Cav.)] |
| title_fullStr | Organogenesis in-vitro using three tissues types of tree tomato [Solanum betaceum (Cav.)] |
| title_full_unstemmed | Organogenesis in-vitro using three tissues types of tree tomato [Solanum betaceum (Cav.)] |
| title_short | Organogenesis in-vitro using three tissues types of tree tomato [Solanum betaceum (Cav.)] |
| title_sort | organogenesis in vitro using three tissues types of tree tomato solanum betaceum cav |
| topic | biotechnology propagation tissue culture plant growth substances biotecnología propagación cultivo de tejidos sustancias de crecimiento vegetal |
| url | https://hdl.handle.net/10568/82859 |
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