Identification of Novel Associations of Candidate Genes with Resistance to Late Blight in Solanum tuberosum Group Phureja
The genetic basis of quantitative disease resistance has been studied in crops for several decades as an alternative to R gene mediated resistance. The most important disease in the potato crop is late blight, caused by the oomycete Phytophthora infestans. Quantitative disease resistance (QDR), as a...
| Autores principales: | , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Frontiers Media
2017
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/82858 |
| _version_ | 1855515167735939072 |
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| author | Álvarez, María Fernanda Angarita, Myrian Delgado, María C. García, Celsa Jiménez Gomez, José Gebhardt, Christiane Mosquera, Teresa |
| author_browse | Angarita, Myrian Delgado, María C. García, Celsa Gebhardt, Christiane Jiménez Gomez, José Mosquera, Teresa Álvarez, María Fernanda |
| author_facet | Álvarez, María Fernanda Angarita, Myrian Delgado, María C. García, Celsa Jiménez Gomez, José Gebhardt, Christiane Mosquera, Teresa |
| author_sort | Álvarez, María Fernanda |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The genetic basis of quantitative disease resistance has been studied in crops for several decades as an alternative to R gene mediated resistance. The most important disease in the potato crop is late blight, caused by the oomycete Phytophthora infestans. Quantitative disease resistance (QDR), as any other quantitative trait in plants, can be genetically mapped to understand the genetic architecture. Association mapping using DNA-based markers has been implemented in many crops to dissect quantitative traits. We used an association mapping approach with candidate genes to identify the first genes associated with quantitative resistance to late blight in Solanum tuberosum Group Phureja. Twenty-nine candidate genes were selected from a set of genes that were differentially expressed during the resistance response to late blight in tetraploid European potato cultivars. The 29 genes were amplified and sequenced in 104 accessions of S. tuberosum Group Phureja from Latin America. We identified 238 SNPs in the selected genes and tested them for association with resistance to late blight. The phenotypic data were obtained under field conditions by determining the area under disease progress curve (AUDPC) in two seasons and in two locations. Two genes were associated with QDR to late blight, a potato homolog of thylakoid lumen 15 kDa protein (StTL15A) and a stem 28 kDa glycoprotein (StGP28). |
| format | Journal Article |
| id | CGSpace82858 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | CGSpace828582025-03-13T09:43:58Z Identification of Novel Associations of Candidate Genes with Resistance to Late Blight in Solanum tuberosum Group Phureja Álvarez, María Fernanda Angarita, Myrian Delgado, María C. García, Celsa Jiménez Gomez, José Gebhardt, Christiane Mosquera, Teresa disease resistance solanum tuberosum pest control genetics mapping resistencia a la enfermedad control de plagas genética The genetic basis of quantitative disease resistance has been studied in crops for several decades as an alternative to R gene mediated resistance. The most important disease in the potato crop is late blight, caused by the oomycete Phytophthora infestans. Quantitative disease resistance (QDR), as any other quantitative trait in plants, can be genetically mapped to understand the genetic architecture. Association mapping using DNA-based markers has been implemented in many crops to dissect quantitative traits. We used an association mapping approach with candidate genes to identify the first genes associated with quantitative resistance to late blight in Solanum tuberosum Group Phureja. Twenty-nine candidate genes were selected from a set of genes that were differentially expressed during the resistance response to late blight in tetraploid European potato cultivars. The 29 genes were amplified and sequenced in 104 accessions of S. tuberosum Group Phureja from Latin America. We identified 238 SNPs in the selected genes and tested them for association with resistance to late blight. The phenotypic data were obtained under field conditions by determining the area under disease progress curve (AUDPC) in two seasons and in two locations. Two genes were associated with QDR to late blight, a potato homolog of thylakoid lumen 15 kDa protein (StTL15A) and a stem 28 kDa glycoprotein (StGP28). 2017-06 2017-07-24T16:27:10Z 2017-07-24T16:27:10Z Journal Article https://hdl.handle.net/10568/82858 en Open Access Frontiers Media Álvarez, María Fernanda; Angarita, Myrian; Delgado, María C.; García, Celsa; Jiménez-Gomez, José; Gebhardt, Christiane; Mosquera, Teresa. 2017. Identification of Novel Associations of Candidate Genes with Resistance to Late Blight in Solanum tuberosum Group Phureja . Frontier in Plant Science 8: 1040. |
| spellingShingle | disease resistance solanum tuberosum pest control genetics mapping resistencia a la enfermedad control de plagas genética Álvarez, María Fernanda Angarita, Myrian Delgado, María C. García, Celsa Jiménez Gomez, José Gebhardt, Christiane Mosquera, Teresa Identification of Novel Associations of Candidate Genes with Resistance to Late Blight in Solanum tuberosum Group Phureja |
| title | Identification of Novel Associations of Candidate Genes with Resistance to Late Blight in Solanum tuberosum Group Phureja |
| title_full | Identification of Novel Associations of Candidate Genes with Resistance to Late Blight in Solanum tuberosum Group Phureja |
| title_fullStr | Identification of Novel Associations of Candidate Genes with Resistance to Late Blight in Solanum tuberosum Group Phureja |
| title_full_unstemmed | Identification of Novel Associations of Candidate Genes with Resistance to Late Blight in Solanum tuberosum Group Phureja |
| title_short | Identification of Novel Associations of Candidate Genes with Resistance to Late Blight in Solanum tuberosum Group Phureja |
| title_sort | identification of novel associations of candidate genes with resistance to late blight in solanum tuberosum group phureja |
| topic | disease resistance solanum tuberosum pest control genetics mapping resistencia a la enfermedad control de plagas genética |
| url | https://hdl.handle.net/10568/82858 |
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