Elucidation of the compatible interaction between banana and Meloidogyne incognita via high-throughput proteome profiling

With a diverse host range, Meloidogyne incognita (root-knot nematode) is listed as one of the most economically important obligate parasites of agriculture. This nematode species establishes permanent feeding sites in plant root systems soon after infestation. A compatible host-nematode interaction...

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Autores principales: Al-Idrus, A., Carpentier, Sebastien C., Ahmad, M.T., Panis, Bartholomeus, Mohamed, Z.
Formato: Journal Article
Lenguaje:Inglés
Publicado: Public Library of Science 2017
Materias:
Acceso en línea:https://hdl.handle.net/10568/82710
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author Al-Idrus, A.
Carpentier, Sebastien C.
Ahmad, M.T.
Panis, Bartholomeus
Mohamed, Z.
author_browse Ahmad, M.T.
Al-Idrus, A.
Carpentier, Sebastien C.
Mohamed, Z.
Panis, Bartholomeus
author_facet Al-Idrus, A.
Carpentier, Sebastien C.
Ahmad, M.T.
Panis, Bartholomeus
Mohamed, Z.
author_sort Al-Idrus, A.
collection Repository of Agricultural Research Outputs (CGSpace)
description With a diverse host range, Meloidogyne incognita (root-knot nematode) is listed as one of the most economically important obligate parasites of agriculture. This nematode species establishes permanent feeding sites in plant root systems soon after infestation. A compatible host-nematode interaction triggers a cascade of morphological and physiological process disruptions of the host, leading to pathogenesis. Such disruption is reflected by altered gene expression in affected cells, detectable using molecular approaches. We employed a high-throughput proteomics approach to elucidate the events involved in a compatible banana- M. incognita interaction. This study serves as the first crucial step in developing natural banana resistance for the purpose of biological-based nematode management programme. We successfully profiled 114 Grand naine root proteins involved in the interaction with M. incognita at the 30th- and 60th- day after inoculation (dai). The abundance of proteins involved in fundamental biological processes, cellular component organisation and stress responses were significantly altered in inoculated root samples. In addition, the abundance of proteins in pathways associated with defence and giant cell maintenance in plants such as phenylpropanoid biosynthesis, glycolysis and citrate cycle were also implicated by the infestation.
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spelling CGSpace827102025-11-12T05:45:45Z Elucidation of the compatible interaction between banana and Meloidogyne incognita via high-throughput proteome profiling Al-Idrus, A. Carpentier, Sebastien C. Ahmad, M.T. Panis, Bartholomeus Mohamed, Z. musa meloidogyne incognito nematoda roots pathogens proteins pest resistence With a diverse host range, Meloidogyne incognita (root-knot nematode) is listed as one of the most economically important obligate parasites of agriculture. This nematode species establishes permanent feeding sites in plant root systems soon after infestation. A compatible host-nematode interaction triggers a cascade of morphological and physiological process disruptions of the host, leading to pathogenesis. Such disruption is reflected by altered gene expression in affected cells, detectable using molecular approaches. We employed a high-throughput proteomics approach to elucidate the events involved in a compatible banana- M. incognita interaction. This study serves as the first crucial step in developing natural banana resistance for the purpose of biological-based nematode management programme. We successfully profiled 114 Grand naine root proteins involved in the interaction with M. incognita at the 30th- and 60th- day after inoculation (dai). The abundance of proteins involved in fundamental biological processes, cellular component organisation and stress responses were significantly altered in inoculated root samples. In addition, the abundance of proteins in pathways associated with defence and giant cell maintenance in plants such as phenylpropanoid biosynthesis, glycolysis and citrate cycle were also implicated by the infestation. 2017-01-01 2017-07-11T07:04:46Z 2017-07-11T07:04:46Z Journal Article https://hdl.handle.net/10568/82710 en Open Access application/pdf Public Library of Science Al-Idrus, A.; Carpentier, S.C.; Ahmad, M.T.; Panis, B.; Mohamed, Z. (2017) Elucidation of the compatible interaction between banana and Meloidogyne incognita via high-throughput proteome profiling. PLoS One 12(6):e0178438 ISSN:1932-6203
spellingShingle musa
meloidogyne incognito
nematoda
roots
pathogens
proteins
pest resistence
Al-Idrus, A.
Carpentier, Sebastien C.
Ahmad, M.T.
Panis, Bartholomeus
Mohamed, Z.
Elucidation of the compatible interaction between banana and Meloidogyne incognita via high-throughput proteome profiling
title Elucidation of the compatible interaction between banana and Meloidogyne incognita via high-throughput proteome profiling
title_full Elucidation of the compatible interaction between banana and Meloidogyne incognita via high-throughput proteome profiling
title_fullStr Elucidation of the compatible interaction between banana and Meloidogyne incognita via high-throughput proteome profiling
title_full_unstemmed Elucidation of the compatible interaction between banana and Meloidogyne incognita via high-throughput proteome profiling
title_short Elucidation of the compatible interaction between banana and Meloidogyne incognita via high-throughput proteome profiling
title_sort elucidation of the compatible interaction between banana and meloidogyne incognita via high throughput proteome profiling
topic musa
meloidogyne incognito
nematoda
roots
pathogens
proteins
pest resistence
url https://hdl.handle.net/10568/82710
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