| Sumario: | During routine surveys conducted from February to July 2015 in the northern guinea savannah
region of Nigeria, sugarcane and itch grass (Rottboellia cochinchinensis) plants showing virus-like
mosaic symptoms were encountered in farmers’ sugarcane fields in Kaduna State. Symptomatic
leaf tissue samples from five randomly selected plants (sugarcane = 4; itch grass = 1) were dried
and stored under CaCl2 at room temperature then shipped to Texas A&M AgriLife Research and
Extension Center, Weslaco, TX, with USDA-APHIS-PPQ permit (P526P-14-04321) for further
analysis. The MagMAX-96 viral RNA isolation kit (Thermo Fisher) was used to isolate total nucleic
acid (TNA) from each sample and from a sample subset consisting of pooled leaf tissue materials
from both plants. TNA aliquot from the pooled sample was subjected to ribosomal RNA depletion
and cDNA library construction using a TruSeq Stranded Total RNA with Ribo-Zero Plant kit
(Illumina), then sequenced on the Illumina NextSeq 500 platform. The raw high-throughput
sequencing (HTS) reads were analyzed as previously described (Alabi et al. 2015), generating
∼43.5 million Illumina reads (76 nucleotides [nt] in length), of which 31,486 de novo assembled
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