The role of molecular markers in breeding for qualitative and quantitative traits of common bean
Improving selection efficiency through indirect selection is an attractive alternative to plant breeders working to improve qualitative and quantitative traits of common bean (Phaseolus vulgaris L). For the successful application of indirect selection, tight linkages must be developed between simple...
| Autores principales: | , |
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| Formato: | Capítulo de libro |
| Lenguaje: | Inglés |
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International Center for Tropical Agriculture
1997
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/81946 |
| _version_ | 1855522550285598720 |
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| author | Kelly, James D. Miklas, Phillip N. |
| author_browse | Kelly, James D. Miklas, Phillip N. |
| author_facet | Kelly, James D. Miklas, Phillip N. |
| author_sort | Kelly, James D. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Improving selection efficiency through indirect selection is an attractive alternative to plant breeders working to improve qualitative and quantitative traits of common bean (Phaseolus vulgaris L). For the successful application of indirect selection, tight linkages must be developed between simple selectable genetic markers and an economically important gene or quantitative trait loci (QTL) controlling a complex trait. Although indirect selection for qualitative traits appears most promising, direct selection for major gene traits may in certain cases be easier, faster and more cost effective. However, indirect selection of race-specific disease resistance genes offers breeders a viable alternative to ensure that favorable gene combinations are present in new cultivars. Simplifying the selection of complex traits using QTL analysis with linked markers would permit selection in the absence of the pathogen or in locations where trait expression is variable. Combining different mechanisms for resistance is only possible using markers when the phenotypic expression is similar after disease inoculation. Random amplified polymorphic DNA (RAPD) markers tightly linked to individual race-specific resistance genes forms the basis for effective indirect selection for durable genetic resistance. Over 30 RAPD markers linked to 17 major genes which condition resistance to four pathogens of common bean nave been found. In addition, markers associated with QTL conditioning resistance to the three diseases (bean golden mosaic virus, common bacterial blight and web blight) of major importance in the tropics have been identified. These tightly linked RAPD markers have potential to facilitate the efficient selection of complex traits and pyramiding of epistemic resistance genes against different pathogens of common bean. Limitations to marker-assisted selection and the value of genetic mapping and QTL analysis in improving the efficiency of bean breeding programs are discussed. |
| format | Book Chapter |
| id | CGSpace81946 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1997 |
| publishDateRange | 1997 |
| publishDateSort | 1997 |
| publisher | International Center for Tropical Agriculture |
| publisherStr | International Center for Tropical Agriculture |
| record_format | dspace |
| spelling | CGSpace819462025-11-05T16:57:42Z The role of molecular markers in breeding for qualitative and quantitative traits of common bean Kelly, James D. Miklas, Phillip N. phaseolus vulgaris breeding genetic markers plant diseases macrophomina phaseolina xanthomonas campestris rhizoctonia solani Improving selection efficiency through indirect selection is an attractive alternative to plant breeders working to improve qualitative and quantitative traits of common bean (Phaseolus vulgaris L). For the successful application of indirect selection, tight linkages must be developed between simple selectable genetic markers and an economically important gene or quantitative trait loci (QTL) controlling a complex trait. Although indirect selection for qualitative traits appears most promising, direct selection for major gene traits may in certain cases be easier, faster and more cost effective. However, indirect selection of race-specific disease resistance genes offers breeders a viable alternative to ensure that favorable gene combinations are present in new cultivars. Simplifying the selection of complex traits using QTL analysis with linked markers would permit selection in the absence of the pathogen or in locations where trait expression is variable. Combining different mechanisms for resistance is only possible using markers when the phenotypic expression is similar after disease inoculation. Random amplified polymorphic DNA (RAPD) markers tightly linked to individual race-specific resistance genes forms the basis for effective indirect selection for durable genetic resistance. Over 30 RAPD markers linked to 17 major genes which condition resistance to four pathogens of common bean nave been found. In addition, markers associated with QTL conditioning resistance to the three diseases (bean golden mosaic virus, common bacterial blight and web blight) of major importance in the tropics have been identified. These tightly linked RAPD markers have potential to facilitate the efficient selection of complex traits and pyramiding of epistemic resistance genes against different pathogens of common bean. Limitations to marker-assisted selection and the value of genetic mapping and QTL analysis in improving the efficiency of bean breeding programs are discussed. Mejorar la eficiencia de selección de caracteres cualitativos y cuantitativos del frijol común (Phaseolus vulgaris L.) a través de la selección indirecta es una opción atractiva para los mejoradores. Para la aplicación exitosa de la selección indirecta, debe desarrollarse los ligamentos estrechos entre los marcadores genéticos simples seleccionables y un gen económicamente importante o un locus de caracter cuantitativo (QTL). Aunque la selección indirecta de caracteres cualitativos parece ser más prometedora, la selección directa de caracteres cualitativos en algunos casos puede ser más fácil, más rápida y más barata. Sin embargo, la selección indirecta de genes de resistencia específica a las razas de un patógeno variable ofrecen a los mejoradores una alternative viable para asegurar que las combinaciones favorables de genes esten presentes en los nuevos cultivares. Simplificando la selección de caracteres complejos usando marcadores ligados a QTL permitirá la selección en ausencia del patógeno o en localidades donde la expresión del caracter de expresión es variable. La combinación de diferentes mecanismos de resistencia es sólo posible usando marcadores cuando la expresión fenotípica es similar a la obtenida después de la inoculación con el patógeno. Los marcadores RAPDs (Random amplified polymorphic DNA) estrechamente ligados a genes individuales de resistencia a razas especificas de un patógeno, forman la base para la selección indirecta y efectiva de una resistencia genética durable. Más de 30 marcadores RAPD ligados a 17 genes principales que controlan la resistencia a cuatro patógenos de frijol común han sido identificados. Además, marcadores asociados con QTL que controlan la resistencia a tres enfermedades (virus de mosaico dorado, bacteriosis común y mustia hilachosa) de gran importancia en los trópicos también han sido identificados. Estos marcadores RAPD estrechamente ligados, pueden ser utilizados en la selección eficiente de caracteres complejos y en la acumulación (piramidar) de genes de resistencia epistática contra diferentes patógenos del frijol común. Las limitaciones de selección con marcadores moleculares, el valor del mapeo genético y del análisis de QTL para mejorar la eficiencia de selección en frijol son discutidos. 1997 2017-06-20T09:00:02Z 2017-06-20T09:00:02Z Book Chapter https://hdl.handle.net/10568/81946 en Open Access application/pdf International Center for Tropical Agriculture Kelly, James D.; Miklas, P.N.. 1997. The role of molecular markers in breeding for qualitative and quantitative traits of common bean . In: Singh, Shree P; Voysest V., Oswaldo (eds.). Taller de Mejoramiento de Frijol para el Siglo XXI: Bases para una Estrategia para América Latina (1996, Cali, Colombia). [Trabajos presentados] . Centro Internacional de Agricultura Tropical (CIAT), Cali, CO. p. 262-279. |
| spellingShingle | phaseolus vulgaris breeding genetic markers plant diseases macrophomina phaseolina xanthomonas campestris rhizoctonia solani Kelly, James D. Miklas, Phillip N. The role of molecular markers in breeding for qualitative and quantitative traits of common bean |
| title | The role of molecular markers in breeding for qualitative and quantitative traits of common bean |
| title_full | The role of molecular markers in breeding for qualitative and quantitative traits of common bean |
| title_fullStr | The role of molecular markers in breeding for qualitative and quantitative traits of common bean |
| title_full_unstemmed | The role of molecular markers in breeding for qualitative and quantitative traits of common bean |
| title_short | The role of molecular markers in breeding for qualitative and quantitative traits of common bean |
| title_sort | role of molecular markers in breeding for qualitative and quantitative traits of common bean |
| topic | phaseolus vulgaris breeding genetic markers plant diseases macrophomina phaseolina xanthomonas campestris rhizoctonia solani |
| url | https://hdl.handle.net/10568/81946 |
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