Role of the chancre in development of immunity to tsetse-transmitted trypanosoma (Nannomonas) congolense in goats

Local skin reactions (chancres) developed in goats at the sites of deposition, by tsetse flies, ofLocal skin reactions (chancres) developed in goats at the sites of deposition, by tsetse flies, of metacyclics of Trypanosoma congolense. The chancres developed much faster and were more pronounced wh...

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Main Author: Taiwo, V.O.
Format: Tesis
Language:Inglés
Published: University of Ibadan 1987
Subjects:
Online Access:https://hdl.handle.net/10568/81587
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author Taiwo, V.O.
author_browse Taiwo, V.O.
author_facet Taiwo, V.O.
author_sort Taiwo, V.O.
collection Repository of Agricultural Research Outputs (CGSpace)
description Local skin reactions (chancres) developed in goats at the sites of deposition, by tsetse flies, ofLocal skin reactions (chancres) developed in goats at the sites of deposition, by tsetse flies, of metacyclics of Trypanosoma congolense. The chancres developed much faster and were more pronounced when ten infected tsetse were allowed to feed on a spot as compared to only one fly per spot. The initial host cellular reaction in the chancre was predominantly polymorphonuclear, followed at the peak of development of the chancre by a predominantly lymphoblastic and plasmacyticreaction. Trypanosomes were found in various stages of division as well as degeneration in chancre biopsies taken at various days post-infection (pj.). Most of the trypanosomes recovered from the chancre tissue fluid were found to bear the same variable surface glycoprotein (VSG) epitopes as the corresponding metacyclics for as long as 13 days p.i., as revealed by indirect immunofluorescence using mouse anti-metacyclic VSG hyperimmune sera and monoclonal antibodies. Immunization of goats with metacyclic trypanosomes, by exposure to infected tsetse bites followed by treatment of the infected goats on day 13 p.i., gave rise to the development of protection to homologous tsetse-transmitted challenge, whilst immunization by intravenous inoculation of the metacyclics did not induce such protection. Chancre formation would thus appear to be vital for the induction of comprehensive immune recognition of the metacyclic variable antigen repertoire deposited in the skin by infected tsetse, and hence development of protective immunity.
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spelling CGSpace815872023-02-15T11:13:27Z Role of the chancre in development of immunity to tsetse-transmitted trypanosoma (Nannomonas) congolense in goats Taiwo, V.O. immunity tsetse flies trypanosoma goats Local skin reactions (chancres) developed in goats at the sites of deposition, by tsetse flies, ofLocal skin reactions (chancres) developed in goats at the sites of deposition, by tsetse flies, of metacyclics of Trypanosoma congolense. The chancres developed much faster and were more pronounced when ten infected tsetse were allowed to feed on a spot as compared to only one fly per spot. The initial host cellular reaction in the chancre was predominantly polymorphonuclear, followed at the peak of development of the chancre by a predominantly lymphoblastic and plasmacyticreaction. Trypanosomes were found in various stages of division as well as degeneration in chancre biopsies taken at various days post-infection (pj.). Most of the trypanosomes recovered from the chancre tissue fluid were found to bear the same variable surface glycoprotein (VSG) epitopes as the corresponding metacyclics for as long as 13 days p.i., as revealed by indirect immunofluorescence using mouse anti-metacyclic VSG hyperimmune sera and monoclonal antibodies. Immunization of goats with metacyclic trypanosomes, by exposure to infected tsetse bites followed by treatment of the infected goats on day 13 p.i., gave rise to the development of protection to homologous tsetse-transmitted challenge, whilst immunization by intravenous inoculation of the metacyclics did not induce such protection. Chancre formation would thus appear to be vital for the induction of comprehensive immune recognition of the metacyclic variable antigen repertoire deposited in the skin by infected tsetse, and hence development of protective immunity. 1987 2017-06-16T09:03:45Z 2017-06-16T09:03:45Z Thesis https://hdl.handle.net/10568/81587 en Limited Access University of Ibadan Taiwo, V. O. 1987. Role of the chancre in development of immunity to tsetse-transmitted trypanosoma (Nannomonas) congolense in goats. MSc thesis in veterinary science. University of Ibadan.
spellingShingle immunity
tsetse flies
trypanosoma
goats
Taiwo, V.O.
Role of the chancre in development of immunity to tsetse-transmitted trypanosoma (Nannomonas) congolense in goats
title Role of the chancre in development of immunity to tsetse-transmitted trypanosoma (Nannomonas) congolense in goats
title_full Role of the chancre in development of immunity to tsetse-transmitted trypanosoma (Nannomonas) congolense in goats
title_fullStr Role of the chancre in development of immunity to tsetse-transmitted trypanosoma (Nannomonas) congolense in goats
title_full_unstemmed Role of the chancre in development of immunity to tsetse-transmitted trypanosoma (Nannomonas) congolense in goats
title_short Role of the chancre in development of immunity to tsetse-transmitted trypanosoma (Nannomonas) congolense in goats
title_sort role of the chancre in development of immunity to tsetse transmitted trypanosoma nannomonas congolense in goats
topic immunity
tsetse flies
trypanosoma
goats
url https://hdl.handle.net/10568/81587
work_keys_str_mv AT taiwovo roleofthechancreindevelopmentofimmunitytotsetsetransmittedtrypanosomanannomonascongolenseingoats