Expression of the Aeluropus littoralis AlSAP Gene Enhances Rice Yield under Field Drought at the Reproductive Stage
We evaluated the yields of Oryza sativa L. ‘Nipponbare’ rice lines expressing a gene encoding an A20/AN1 domain stress-associated protein, AlSAP, from the halophyte grass Aeluropus littoralis under the control of different promoters. Three independent field trials were conducted, with drought impose...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Frontiers Media
2017
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/81532 |
| _version_ | 1855515866396884992 |
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| author | Ghneim-Herrera, Thaura Selvaraj, Michael Gomez Meynard, Donaldo Fabre, Denis Peña, Alexandra Romdhane, Walid Ben Saad, Rania Ben Ogawa, Satoshi Rebolledo, María Camila Ishitani, Manabu Tohme, Joseph M. Al-Doss, Abdullah Guiderdoni, Emmanuel Hassairi, Afif |
| author_browse | Al-Doss, Abdullah Fabre, Denis Ghneim-Herrera, Thaura Guiderdoni, Emmanuel Hassairi, Afif Ishitani, Manabu Meynard, Donaldo Ogawa, Satoshi Peña, Alexandra Rebolledo, María Camila Romdhane, Walid Ben Saad, Rania Ben Selvaraj, Michael Gomez Tohme, Joseph M. |
| author_facet | Ghneim-Herrera, Thaura Selvaraj, Michael Gomez Meynard, Donaldo Fabre, Denis Peña, Alexandra Romdhane, Walid Ben Saad, Rania Ben Ogawa, Satoshi Rebolledo, María Camila Ishitani, Manabu Tohme, Joseph M. Al-Doss, Abdullah Guiderdoni, Emmanuel Hassairi, Afif |
| author_sort | Ghneim-Herrera, Thaura |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | We evaluated the yields of Oryza sativa L. ‘Nipponbare’ rice lines expressing a gene encoding an A20/AN1 domain stress-associated protein, AlSAP, from the halophyte grass Aeluropus littoralis under the control of different promoters. Three independent field trials were conducted, with drought imposed at the reproductive stage. In all trials, the two transgenic lines, RN5 and RN6, consistently out-performed non-transgenic (NT) and wild-type (WT) controls, providing 50–90% increases in grain yield (GY). Enhancement of tillering and panicle fertility contributed to this improved GY under drought. In contrast with physiological records collected during previous greenhouse dry-down experiments, where drought was imposed at the early tillering stage, we did not observe significant differences in photosynthetic parameters, leaf water potential, or accumulation of antioxidants in flag leaves of AlSAP-lines subjected to drought at flowering. However, AlSAP expression alleviated leaf rolling and leaf drying induced by drought, resulting in increased accumulation of green biomass. Therefore, the observed enhanced performance of the AlSAP-lines subjected to drought at the reproductive stage can be tentatively ascribed to a primed status of the transgenic plants, resulting from a higher accumulation of biomass during vegetative growth, allowing reserve remobilization and maintenance of productive tillering and grain filling. Under irrigated conditions, the overall performance of AlSAP-lines was comparable with, or even significantly better than, the NT and WT controls. Thus, AlSAP expression inflicted no penalty on rice yields under optimal growth conditions. Our results support the use of AlSAP transgenics to reduce rice GY losses under drought conditions. |
| format | Journal Article |
| id | CGSpace81532 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | CGSpace815322025-03-13T09:43:59Z Expression of the Aeluropus littoralis AlSAP Gene Enhances Rice Yield under Field Drought at the Reproductive Stage Ghneim-Herrera, Thaura Selvaraj, Michael Gomez Meynard, Donaldo Fabre, Denis Peña, Alexandra Romdhane, Walid Ben Saad, Rania Ben Ogawa, Satoshi Rebolledo, María Camila Ishitani, Manabu Tohme, Joseph M. Al-Doss, Abdullah Guiderdoni, Emmanuel Hassairi, Afif oryza sativa rice drought stress drought tolerance transgénicos transgenics yield arroz estrés de sequia rendimiento We evaluated the yields of Oryza sativa L. ‘Nipponbare’ rice lines expressing a gene encoding an A20/AN1 domain stress-associated protein, AlSAP, from the halophyte grass Aeluropus littoralis under the control of different promoters. Three independent field trials were conducted, with drought imposed at the reproductive stage. In all trials, the two transgenic lines, RN5 and RN6, consistently out-performed non-transgenic (NT) and wild-type (WT) controls, providing 50–90% increases in grain yield (GY). Enhancement of tillering and panicle fertility contributed to this improved GY under drought. In contrast with physiological records collected during previous greenhouse dry-down experiments, where drought was imposed at the early tillering stage, we did not observe significant differences in photosynthetic parameters, leaf water potential, or accumulation of antioxidants in flag leaves of AlSAP-lines subjected to drought at flowering. However, AlSAP expression alleviated leaf rolling and leaf drying induced by drought, resulting in increased accumulation of green biomass. Therefore, the observed enhanced performance of the AlSAP-lines subjected to drought at the reproductive stage can be tentatively ascribed to a primed status of the transgenic plants, resulting from a higher accumulation of biomass during vegetative growth, allowing reserve remobilization and maintenance of productive tillering and grain filling. Under irrigated conditions, the overall performance of AlSAP-lines was comparable with, or even significantly better than, the NT and WT controls. Thus, AlSAP expression inflicted no penalty on rice yields under optimal growth conditions. Our results support the use of AlSAP transgenics to reduce rice GY losses under drought conditions. 2017 2017-06-12T21:23:13Z 2017-06-12T21:23:13Z Journal Article https://hdl.handle.net/10568/81532 en Open Access Frontiers Media Ghneim-Herrera, Thaura; Gomez Selvaraj, Michael; Meynard, Donaldo; Fabre, Denis; Peña, Alexandra; Romdhane, Walid Ben; Saad, Rania Ben; Ogawa, Satoshi; Rebolledo, Maria C.; Ishitani, Manabu; Tohme, Joe; Al-Doss, Abdullah; Guiderdoni, Emmanuel; Hassairi, Afif. 2017. Expression of the Aeluropus littoralis AlSAP Gene Enhances Rice Yield under Field Drought at the Reproductive Stage . Frontiers in Plant Science . 8: 994. https://doi.org/10.3389/fpls.2017.00994 |
| spellingShingle | oryza sativa rice drought stress drought tolerance transgénicos transgenics yield arroz estrés de sequia rendimiento Ghneim-Herrera, Thaura Selvaraj, Michael Gomez Meynard, Donaldo Fabre, Denis Peña, Alexandra Romdhane, Walid Ben Saad, Rania Ben Ogawa, Satoshi Rebolledo, María Camila Ishitani, Manabu Tohme, Joseph M. Al-Doss, Abdullah Guiderdoni, Emmanuel Hassairi, Afif Expression of the Aeluropus littoralis AlSAP Gene Enhances Rice Yield under Field Drought at the Reproductive Stage |
| title | Expression of the Aeluropus littoralis AlSAP Gene Enhances Rice Yield under Field Drought at the Reproductive Stage |
| title_full | Expression of the Aeluropus littoralis AlSAP Gene Enhances Rice Yield under Field Drought at the Reproductive Stage |
| title_fullStr | Expression of the Aeluropus littoralis AlSAP Gene Enhances Rice Yield under Field Drought at the Reproductive Stage |
| title_full_unstemmed | Expression of the Aeluropus littoralis AlSAP Gene Enhances Rice Yield under Field Drought at the Reproductive Stage |
| title_short | Expression of the Aeluropus littoralis AlSAP Gene Enhances Rice Yield under Field Drought at the Reproductive Stage |
| title_sort | expression of the aeluropus littoralis alsap gene enhances rice yield under field drought at the reproductive stage |
| topic | oryza sativa rice drought stress drought tolerance transgénicos transgenics yield arroz estrés de sequia rendimiento |
| url | https://hdl.handle.net/10568/81532 |
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