Sensitivity of Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidea) to the Entomopathogenic fungi, Metarhizium anisopliae and Beauveria bassiana in laboratory.

The present work aims to assess the pathogenicity of the conidia of six isolates of Metarhizium anisopliae and two isolates of Beauveria bassiana on Helicoverpa armigera in order to select the most promising ones for developing a microbiological product to be used in cotton fields. The pathogenicity...

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Main Authors: Douro-Kpindou, O.K., Djegui, D.A., Glitho, I.A., Tamo, Manuele
Format: Journal Article
Language:Inglés
Published: 2012
Subjects:
Online Access:https://hdl.handle.net/10568/80862
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author Douro-Kpindou, O.K.
Djegui, D.A.
Glitho, I.A.
Tamo, Manuele
author_browse Djegui, D.A.
Douro-Kpindou, O.K.
Glitho, I.A.
Tamo, Manuele
author_facet Douro-Kpindou, O.K.
Djegui, D.A.
Glitho, I.A.
Tamo, Manuele
author_sort Douro-Kpindou, O.K.
collection Repository of Agricultural Research Outputs (CGSpace)
description The present work aims to assess the pathogenicity of the conidia of six isolates of Metarhizium anisopliae and two isolates of Beauveria bassiana on Helicoverpa armigera in order to select the most promising ones for developing a microbiological product to be used in cotton fields. The pathogenicity of six isolates of the entomopathogenic fungi, M. anisopliae (Met 28, Met 32, Met 92, Met 31, Met 34 and Met 341) and two isolates of B. bassiana (Bb11 and Bb 12) was evaluated in the laboratory by applying topically 1 μl of an oil-based formulation of conidia at a concentration of 108 conidia/ml on the third, fourth, fifth and the sixth instars of H. armigera. Taken separately, each isolate caused less mortality among the larvae of the sixth stage than in younger stages. Corrected mortality rates varied from 3.6 ± 1.8 % (Met 28, sixth instar) to 56.3 ± 0.8% (Met 31, third instar) for M. anisopliae and from 3.6 ± 1.8 % (Bb 12, sixth instar) to 34.4 ± 4.2% (Bb 11, third instar) for B. bassiana. Certain isolates of Metarhizium were infective to pupa. Host development stage at inoculation affected both survival times compared to control. The results of the presented study showed that the isolates of Metarhizium (Met 31) and Beauveria (Bb 11, known as Bba 5653), the last being tested successfully against Plutella xylostella (Lepidoptera: Noctuidae), are virulent isolates and can be promising isolates for the control of the cotton bollworm H. armigera.
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spelling CGSpace808622023-02-15T06:46:45Z Sensitivity of Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidea) to the Entomopathogenic fungi, Metarhizium anisopliae and Beauveria bassiana in laboratory. Douro-Kpindou, O.K. Djegui, D.A. Glitho, I.A. Tamo, Manuele helicoverpa armigera microbial control entomopathogenic fungi metarhizium anisopliae beauveria bassiana pathogenicity The present work aims to assess the pathogenicity of the conidia of six isolates of Metarhizium anisopliae and two isolates of Beauveria bassiana on Helicoverpa armigera in order to select the most promising ones for developing a microbiological product to be used in cotton fields. The pathogenicity of six isolates of the entomopathogenic fungi, M. anisopliae (Met 28, Met 32, Met 92, Met 31, Met 34 and Met 341) and two isolates of B. bassiana (Bb11 and Bb 12) was evaluated in the laboratory by applying topically 1 μl of an oil-based formulation of conidia at a concentration of 108 conidia/ml on the third, fourth, fifth and the sixth instars of H. armigera. Taken separately, each isolate caused less mortality among the larvae of the sixth stage than in younger stages. Corrected mortality rates varied from 3.6 ± 1.8 % (Met 28, sixth instar) to 56.3 ± 0.8% (Met 31, third instar) for M. anisopliae and from 3.6 ± 1.8 % (Bb 12, sixth instar) to 34.4 ± 4.2% (Bb 11, third instar) for B. bassiana. Certain isolates of Metarhizium were infective to pupa. Host development stage at inoculation affected both survival times compared to control. The results of the presented study showed that the isolates of Metarhizium (Met 31) and Beauveria (Bb 11, known as Bba 5653), the last being tested successfully against Plutella xylostella (Lepidoptera: Noctuidae), are virulent isolates and can be promising isolates for the control of the cotton bollworm H. armigera. 2012-12 2017-05-03T08:45:46Z 2017-05-03T08:45:46Z Journal Article https://hdl.handle.net/10568/80862 en Limited Access Douro Kpindou, O., Djegui, D., Glitho, I.A. & Tamo, M. (2012). Sensitivity of Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidea) to the Entomopathogenic fungi, Metarhizium anisopliae and Beauveria bassiana in laboratory. ARPN Journal of Agricultural and Biological Science, 7(12), 1007-1015.
spellingShingle helicoverpa armigera
microbial
control
entomopathogenic fungi
metarhizium anisopliae
beauveria bassiana
pathogenicity
Douro-Kpindou, O.K.
Djegui, D.A.
Glitho, I.A.
Tamo, Manuele
Sensitivity of Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidea) to the Entomopathogenic fungi, Metarhizium anisopliae and Beauveria bassiana in laboratory.
title Sensitivity of Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidea) to the Entomopathogenic fungi, Metarhizium anisopliae and Beauveria bassiana in laboratory.
title_full Sensitivity of Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidea) to the Entomopathogenic fungi, Metarhizium anisopliae and Beauveria bassiana in laboratory.
title_fullStr Sensitivity of Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidea) to the Entomopathogenic fungi, Metarhizium anisopliae and Beauveria bassiana in laboratory.
title_full_unstemmed Sensitivity of Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidea) to the Entomopathogenic fungi, Metarhizium anisopliae and Beauveria bassiana in laboratory.
title_short Sensitivity of Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidea) to the Entomopathogenic fungi, Metarhizium anisopliae and Beauveria bassiana in laboratory.
title_sort sensitivity of helicoverpa armigera hubner lepidoptera noctuidea to the entomopathogenic fungi metarhizium anisopliae and beauveria bassiana in laboratory
topic helicoverpa armigera
microbial
control
entomopathogenic fungi
metarhizium anisopliae
beauveria bassiana
pathogenicity
url https://hdl.handle.net/10568/80862
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