Fine mapping of RYMV3: a new resistance gene to Rice yellow mottle virus from Oryza glaberrima
Rice yellow mottle virus (RYMV) disease is a serious constraint to the cultivation of rice in Africa and selection for resistance is considered to be the most effective management strategy. The aim of this study was to characterize the resistance of Tog5307, a highly resistant accession belonging to...
| Autores principales: | , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2017
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/80708 |
| _version_ | 1855520150868983808 |
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| author | Pidon, H. Ghesquière, Alain Chéron, S. Issaka, S. Hébrard, E. Sabot, François Kolade, O. Silué, D. Albar, L. |
| author_browse | Albar, L. Chéron, S. Ghesquière, Alain Hébrard, E. Issaka, S. Kolade, O. Pidon, H. Sabot, François Silué, D. |
| author_facet | Pidon, H. Ghesquière, Alain Chéron, S. Issaka, S. Hébrard, E. Sabot, François Kolade, O. Silué, D. Albar, L. |
| author_sort | Pidon, H. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Rice yellow mottle virus (RYMV) disease is a serious constraint to the cultivation of rice in Africa and selection for resistance is considered to be the most effective management strategy. The aim of this study was to characterize the resistance of Tog5307, a highly resistant accession belonging to the African cultivated rice species (Oryza glaberrima), that has none of the previously identified resistance genes to RYMV. The specificity of Tog5307 resistance was analyzed using 18 RYMV isolates. While three of them were able to infect Tog5307 very rapidly, resistance against the others was effective despite infection events attributed to resistance-breakdown or incomplete penetrance of the resistance. Segregation of resistance in an interspecific backcross population derived from a cross between Tog5307 and the susceptible Oryza sativa variety IR64 showed that resistance is dominant and is controlled by a single gene, named RYMV3. RYMV3 was mapped in an approximately 15-kb interval in which two candidate genes, coding for a putative transmembrane protein and a CC-NBS-LRR domain-containing protein, were annotated. Sequencing revealed non-synonymous polymorphisms between Tog5307 and the O. glaberrima susceptible accession CG14 in both candidate genes. An additional resistant O. glaberrima accession, Tog5672, was found to have the Tog5307 genotype for the CC-NBS-LRR gene but not for the putative transmembrane protein gene. Analysis of the cosegregation of Tog5672 resistance with the RYMV3 locus suggests that RYMV3 is also involved in Tog5672 resistance, thereby supporting the CC-NBS-LRR gene as the best candidate for RYMV3. |
| format | Journal Article |
| id | CGSpace80708 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace807082025-01-28T07:08:05Z Fine mapping of RYMV3: a new resistance gene to Rice yellow mottle virus from Oryza glaberrima Pidon, H. Ghesquière, Alain Chéron, S. Issaka, S. Hébrard, E. Sabot, François Kolade, O. Silué, D. Albar, L. rice- oryza fine mapping rymv rice oryza glaberrima resistance nb-lrr resistance genes genetics biotechnology Rice yellow mottle virus (RYMV) disease is a serious constraint to the cultivation of rice in Africa and selection for resistance is considered to be the most effective management strategy. The aim of this study was to characterize the resistance of Tog5307, a highly resistant accession belonging to the African cultivated rice species (Oryza glaberrima), that has none of the previously identified resistance genes to RYMV. The specificity of Tog5307 resistance was analyzed using 18 RYMV isolates. While three of them were able to infect Tog5307 very rapidly, resistance against the others was effective despite infection events attributed to resistance-breakdown or incomplete penetrance of the resistance. Segregation of resistance in an interspecific backcross population derived from a cross between Tog5307 and the susceptible Oryza sativa variety IR64 showed that resistance is dominant and is controlled by a single gene, named RYMV3. RYMV3 was mapped in an approximately 15-kb interval in which two candidate genes, coding for a putative transmembrane protein and a CC-NBS-LRR domain-containing protein, were annotated. Sequencing revealed non-synonymous polymorphisms between Tog5307 and the O. glaberrima susceptible accession CG14 in both candidate genes. An additional resistant O. glaberrima accession, Tog5672, was found to have the Tog5307 genotype for the CC-NBS-LRR gene but not for the putative transmembrane protein gene. Analysis of the cosegregation of Tog5672 resistance with the RYMV3 locus suggests that RYMV3 is also involved in Tog5672 resistance, thereby supporting the CC-NBS-LRR gene as the best candidate for RYMV3. 2017-04 2017-04-12T09:16:17Z 2017-04-12T09:16:17Z Journal Article https://hdl.handle.net/10568/80708 en Limited Access Springer Pidon, H., Ghesquière, A., Chéron, S., Issaka, S., Hébrard, E., Sabot, F., ... & Albar, L. (2017). Fine mapping of RYMV3: a new resistance gene to Rice yellow mottle virus from Oryza glaberrima. Theoretical and Applied Genetics, 130(4), 807-818. |
| spellingShingle | rice- oryza fine mapping rymv rice oryza glaberrima resistance nb-lrr resistance genes genetics biotechnology Pidon, H. Ghesquière, Alain Chéron, S. Issaka, S. Hébrard, E. Sabot, François Kolade, O. Silué, D. Albar, L. Fine mapping of RYMV3: a new resistance gene to Rice yellow mottle virus from Oryza glaberrima |
| title | Fine mapping of RYMV3: a new resistance gene to Rice yellow mottle virus from Oryza glaberrima |
| title_full | Fine mapping of RYMV3: a new resistance gene to Rice yellow mottle virus from Oryza glaberrima |
| title_fullStr | Fine mapping of RYMV3: a new resistance gene to Rice yellow mottle virus from Oryza glaberrima |
| title_full_unstemmed | Fine mapping of RYMV3: a new resistance gene to Rice yellow mottle virus from Oryza glaberrima |
| title_short | Fine mapping of RYMV3: a new resistance gene to Rice yellow mottle virus from Oryza glaberrima |
| title_sort | fine mapping of rymv3 a new resistance gene to rice yellow mottle virus from oryza glaberrima |
| topic | rice- oryza fine mapping rymv rice oryza glaberrima resistance nb-lrr resistance genes genetics biotechnology |
| url | https://hdl.handle.net/10568/80708 |
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