Overexpression of an Arabidopsis thaliana galactinol synthase gene improves drought tolerance in transgenic rice and increased grain yield in the field
Drought stress has often caused significant decreases in crop production which could be associated with global warming. Enhancing drought tolerance without a grain yield penalty has been a great challenge in crop improvement. Here, we report the Arabidopsis thaliana galactinol synthase 2 gene (AtGol...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Wiley
2017
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/80683 |
| _version_ | 1855522625584889856 |
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| author | Selvaraj, Michael Gomez Ishizaki, Takuma Valencia Ortiz, Milton Orlando Ogawa, Satoshi Dedicova, Beata Ogata, Takuya Yoshiwara, Kyouko Maruyama, Kyonoshin Kusano, Miyako Saito, Kazuki Takahashi, Fuminori Shinozaki, Kazuo Nakashima, Kazuo Ishitani, Manabu |
| author_browse | Dedicova, Beata Ishitani, Manabu Ishizaki, Takuma Kusano, Miyako Maruyama, Kyonoshin Nakashima, Kazuo Ogata, Takuya Ogawa, Satoshi Saito, Kazuki Selvaraj, Michael Gomez Shinozaki, Kazuo Takahashi, Fuminori Valencia Ortiz, Milton Orlando Yoshiwara, Kyouko |
| author_facet | Selvaraj, Michael Gomez Ishizaki, Takuma Valencia Ortiz, Milton Orlando Ogawa, Satoshi Dedicova, Beata Ogata, Takuya Yoshiwara, Kyouko Maruyama, Kyonoshin Kusano, Miyako Saito, Kazuki Takahashi, Fuminori Shinozaki, Kazuo Nakashima, Kazuo Ishitani, Manabu |
| author_sort | Selvaraj, Michael Gomez |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Drought stress has often caused significant decreases in crop production which could be associated with global warming. Enhancing drought tolerance without a grain yield penalty has been a great challenge in crop improvement. Here, we report the Arabidopsis thaliana galactinol synthase 2 gene (AtGolS2) was able to confer drought tolerance and increase grain yield in two different rice (Oryza sativa) genotypes under dry field conditions. The developed Ubi:AtGolS2 transgenic lines also had higher levels of galactinol than the non-transgenic control. The increased grain yield of the transgenic rice under drought conditions was related to a higher number of panicles, grain fertility and biomass. Extensive confined field trials using transgenic lines expressing AtGolS2 under the control of the constitutive maize ubiquitin promoter (Ubi:AtGolS2) in Curinga, tropical japonica and NERICA4, interspecific hybrid across two different seasons and environments revealed the verified lines have the proven field drought tolerance of the Ubi:AtGolS2 transgenic rice. The amended drought tolerance was associated with higher relative water content of leaves, higher photosynthesis activity, lesser reduction in plant growth and faster recovering ability. Collectively, our results provide strong evidence that AtGolS2 is a useful biotechnological tool to reduce grain yield losses in rice beyond genetic differences under field drought stress. |
| format | Journal Article |
| id | CGSpace80683 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace806832025-03-13T09:44:09Z Overexpression of an Arabidopsis thaliana galactinol synthase gene improves drought tolerance in transgenic rice and increased grain yield in the field Selvaraj, Michael Gomez Ishizaki, Takuma Valencia Ortiz, Milton Orlando Ogawa, Satoshi Dedicova, Beata Ogata, Takuya Yoshiwara, Kyouko Maruyama, Kyonoshin Kusano, Miyako Saito, Kazuki Takahashi, Fuminori Shinozaki, Kazuo Nakashima, Kazuo Ishitani, Manabu arabidopsis thaliana oryza sativa rice drought stress drought tolerance genotypes transgenics yield arroz estrés de sequia transgénicos rendimiento plant breeding fitomejoramiento Drought stress has often caused significant decreases in crop production which could be associated with global warming. Enhancing drought tolerance without a grain yield penalty has been a great challenge in crop improvement. Here, we report the Arabidopsis thaliana galactinol synthase 2 gene (AtGolS2) was able to confer drought tolerance and increase grain yield in two different rice (Oryza sativa) genotypes under dry field conditions. The developed Ubi:AtGolS2 transgenic lines also had higher levels of galactinol than the non-transgenic control. The increased grain yield of the transgenic rice under drought conditions was related to a higher number of panicles, grain fertility and biomass. Extensive confined field trials using transgenic lines expressing AtGolS2 under the control of the constitutive maize ubiquitin promoter (Ubi:AtGolS2) in Curinga, tropical japonica and NERICA4, interspecific hybrid across two different seasons and environments revealed the verified lines have the proven field drought tolerance of the Ubi:AtGolS2 transgenic rice. The amended drought tolerance was associated with higher relative water content of leaves, higher photosynthesis activity, lesser reduction in plant growth and faster recovering ability. Collectively, our results provide strong evidence that AtGolS2 is a useful biotechnological tool to reduce grain yield losses in rice beyond genetic differences under field drought stress. 2017-11 2017-04-07T19:47:26Z 2017-04-07T19:47:26Z Journal Article https://hdl.handle.net/10568/80683 en Open Access Wiley Gomez Selvaraj, Michael; Ishizaki, Takuma; Valencia, Milton; Ogawa, Satoshi; Dedicova, Beata; Ogata, Takuya; Yoshiwara, Kyouko; Maruyama, Kyonoshin; Kusano, Miyako; Saito, Kazuki; Takahashi, Fuminori; Shinozaki, Kazuo; Nakashima, Kazuo; Ishitani, Manabu. 2017. Overexpression of an Arabidopsis thaliana galactinol synthase gene improves drought tolerance in transgenic rice and increased grain yield in the field . Plant Biotechnology Journal .15(11): 1465-1477. |
| spellingShingle | arabidopsis thaliana oryza sativa rice drought stress drought tolerance genotypes transgenics yield arroz estrés de sequia transgénicos rendimiento plant breeding fitomejoramiento Selvaraj, Michael Gomez Ishizaki, Takuma Valencia Ortiz, Milton Orlando Ogawa, Satoshi Dedicova, Beata Ogata, Takuya Yoshiwara, Kyouko Maruyama, Kyonoshin Kusano, Miyako Saito, Kazuki Takahashi, Fuminori Shinozaki, Kazuo Nakashima, Kazuo Ishitani, Manabu Overexpression of an Arabidopsis thaliana galactinol synthase gene improves drought tolerance in transgenic rice and increased grain yield in the field |
| title | Overexpression of an Arabidopsis thaliana galactinol synthase gene improves drought tolerance in transgenic rice and increased grain yield in the field |
| title_full | Overexpression of an Arabidopsis thaliana galactinol synthase gene improves drought tolerance in transgenic rice and increased grain yield in the field |
| title_fullStr | Overexpression of an Arabidopsis thaliana galactinol synthase gene improves drought tolerance in transgenic rice and increased grain yield in the field |
| title_full_unstemmed | Overexpression of an Arabidopsis thaliana galactinol synthase gene improves drought tolerance in transgenic rice and increased grain yield in the field |
| title_short | Overexpression of an Arabidopsis thaliana galactinol synthase gene improves drought tolerance in transgenic rice and increased grain yield in the field |
| title_sort | overexpression of an arabidopsis thaliana galactinol synthase gene improves drought tolerance in transgenic rice and increased grain yield in the field |
| topic | arabidopsis thaliana oryza sativa rice drought stress drought tolerance genotypes transgenics yield arroz estrés de sequia transgénicos rendimiento plant breeding fitomejoramiento |
| url | https://hdl.handle.net/10568/80683 |
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