Non-classical gluconeogenesis-dependent glucose metabolism in Rhipicephalus microplus embryonic cell line BME26
In this work we evaluated several genes involved in gluconeogenesis, glycolysis and glycogen metabolism, the major pathways for carbohydrate catabolism and anabolism, in the BME26 Rhipicephalus microplus embryonic cell line. Genetic and catalytic control of the genes and enzymes associated with thes...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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MDPI
2015
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| Online Access: | https://hdl.handle.net/10568/80654 |
| _version_ | 1855517286378504192 |
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| author | Silva, R.M. da Noce, B.D. Waltero, C.F. Costa, E.P. Abreu, L.A. de Githaka, Naftaly W. Moraes, J. Gomes, H.F. Konnai, S. Silva Vaz, I. da Ohashi, K. Logullo, C. |
| author_browse | Abreu, L.A. de Costa, E.P. Githaka, Naftaly W. Gomes, H.F. Konnai, S. Logullo, C. Moraes, J. Noce, B.D. Ohashi, K. Silva Vaz, I. da Silva, R.M. da Waltero, C.F. |
| author_facet | Silva, R.M. da Noce, B.D. Waltero, C.F. Costa, E.P. Abreu, L.A. de Githaka, Naftaly W. Moraes, J. Gomes, H.F. Konnai, S. Silva Vaz, I. da Ohashi, K. Logullo, C. |
| author_sort | Silva, R.M. da |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | In this work we evaluated several genes involved in gluconeogenesis, glycolysis and glycogen metabolism, the major pathways for carbohydrate catabolism and anabolism, in the BME26 Rhipicephalus microplus embryonic cell line. Genetic and catalytic control of the genes and enzymes associated with these pathways are modulated by alterations in energy resource availability (primarily glucose). BME26 cells in media were investigated using three different glucose concentrations, and changes in the transcription levels of target genes in response to carbohydrate utilization were assessed. The results indicate that several genes, such as glycogen synthase (GS), glycogen synthase kinase 3 (GSK3), phosphoenolpyruvate carboxykinase (PEPCK), and glucose-6 phosphatase (GP) displayed mutual regulation in response to glucose treatment. Surprisingly, the transcription of gluconeogenic enzymes was found to increase alongside that of glycolytic enzymes, especially pyruvate kinase, with high glucose treatment. In addition, RNAi data from this study revealed that the transcription of gluconeogenic genes in BME26 cells is controlled by GSK-3. Collectively, these results improve our understanding of how glucose metabolism is regulated at the genetic level in tick cells. |
| format | Journal Article |
| id | CGSpace80654 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2015 |
| publishDateRange | 2015 |
| publishDateSort | 2015 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | CGSpace806542025-12-08T09:54:28Z Non-classical gluconeogenesis-dependent glucose metabolism in Rhipicephalus microplus embryonic cell line BME26 Silva, R.M. da Noce, B.D. Waltero, C.F. Costa, E.P. Abreu, L.A. de Githaka, Naftaly W. Moraes, J. Gomes, H.F. Konnai, S. Silva Vaz, I. da Ohashi, K. Logullo, C. anabolism animal disease catabolism cattle genes glucose metabolism spectroscopy molecular biology catalysis In this work we evaluated several genes involved in gluconeogenesis, glycolysis and glycogen metabolism, the major pathways for carbohydrate catabolism and anabolism, in the BME26 Rhipicephalus microplus embryonic cell line. Genetic and catalytic control of the genes and enzymes associated with these pathways are modulated by alterations in energy resource availability (primarily glucose). BME26 cells in media were investigated using three different glucose concentrations, and changes in the transcription levels of target genes in response to carbohydrate utilization were assessed. The results indicate that several genes, such as glycogen synthase (GS), glycogen synthase kinase 3 (GSK3), phosphoenolpyruvate carboxykinase (PEPCK), and glucose-6 phosphatase (GP) displayed mutual regulation in response to glucose treatment. Surprisingly, the transcription of gluconeogenic enzymes was found to increase alongside that of glycolytic enzymes, especially pyruvate kinase, with high glucose treatment. In addition, RNAi data from this study revealed that the transcription of gluconeogenic genes in BME26 cells is controlled by GSK-3. Collectively, these results improve our understanding of how glucose metabolism is regulated at the genetic level in tick cells. 2015-01-14 2017-04-05T10:31:11Z 2017-04-05T10:31:11Z Journal Article https://hdl.handle.net/10568/80654 en Open Access MDPI Silva, R.M. da, Noce, B.D., Waltero, C.F., Costa, E.P., Abreu, L.A. de, Githaka, N.W., Moraes, J., Gomes, H.F., Konnai, S., Silva Vaz Jr., I. Da, Ohashi, K. and Logullo, C., 2015. Non-classical gluconeogenesis-dependent glucose metabolism in Rhipicephalus microplus embryonic cell line BME26. International Journal of Molecular Sciences 16(1): 1821-1839. |
| spellingShingle | anabolism animal disease catabolism cattle genes glucose metabolism spectroscopy molecular biology catalysis Silva, R.M. da Noce, B.D. Waltero, C.F. Costa, E.P. Abreu, L.A. de Githaka, Naftaly W. Moraes, J. Gomes, H.F. Konnai, S. Silva Vaz, I. da Ohashi, K. Logullo, C. Non-classical gluconeogenesis-dependent glucose metabolism in Rhipicephalus microplus embryonic cell line BME26 |
| title | Non-classical gluconeogenesis-dependent glucose metabolism in Rhipicephalus microplus embryonic cell line BME26 |
| title_full | Non-classical gluconeogenesis-dependent glucose metabolism in Rhipicephalus microplus embryonic cell line BME26 |
| title_fullStr | Non-classical gluconeogenesis-dependent glucose metabolism in Rhipicephalus microplus embryonic cell line BME26 |
| title_full_unstemmed | Non-classical gluconeogenesis-dependent glucose metabolism in Rhipicephalus microplus embryonic cell line BME26 |
| title_short | Non-classical gluconeogenesis-dependent glucose metabolism in Rhipicephalus microplus embryonic cell line BME26 |
| title_sort | non classical gluconeogenesis dependent glucose metabolism in rhipicephalus microplus embryonic cell line bme26 |
| topic | anabolism animal disease catabolism cattle genes glucose metabolism spectroscopy molecular biology catalysis |
| url | https://hdl.handle.net/10568/80654 |
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