QTL identification for cassava bacterial blight resistance under natural infection conditions
Cassava, *Manihot esculenta* Crantz, represents the main food source for more than one billion people. Cassava's production is affected by several diseases, one of the most serious is cassava bacterial blight (CBB) caused by *Xanthomonas axonopodis* pv. *manihotis* (*Xam*). A quantitative trait loci...
| Main Authors: | , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Universidad Nacional de Colombia
2017
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/80479 |
| _version_ | 1855535414810509312 |
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| author | Soto Sedano, Johana Mora Moreno, Rubén Eduardo Calle, Fernando López Carrascal, Camilo Ernesto |
| author_browse | Calle, Fernando López Carrascal, Camilo Ernesto Mora Moreno, Rubén Eduardo Soto Sedano, Johana |
| author_facet | Soto Sedano, Johana Mora Moreno, Rubén Eduardo Calle, Fernando López Carrascal, Camilo Ernesto |
| author_sort | Soto Sedano, Johana |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Cassava, *Manihot esculenta* Crantz, represents the main food source for more than one billion people. Cassava's production is affected by several diseases, one of the most serious is cassava bacterial blight (CBB) caused by *Xanthomonas axonopodis* pv. *manihotis* (*Xam*). A quantitative trait loci (QTL) analysis for CBB resistance was performed under natural infection conditions, using a mapping population of 99 full-sibs genotypes highly segregant and a SNP-based high dense genetic map. The phenotypic evaluation was carried out in Puerto López, Meta, Colombia, during the rainy season in 2015. Both resistant and susceptible transgressive segregants were detected in the mapping population. Through a non-parametric interval mapping analysis, two QTL were detected, explaining 10.9 and 12.6 % of phenotypic variance of resistance to field CBB. After a bioinformatics exploration four genes were identified in the QTL intervals. This work represents a contribution to the elucidation of the molecular bases of quantitative cassava resistance to *Xam*. |
| format | Journal Article |
| id | CGSpace80479 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| publisher | Universidad Nacional de Colombia |
| publisherStr | Universidad Nacional de Colombia |
| record_format | dspace |
| spelling | CGSpace804792025-03-13T09:45:00Z QTL identification for cassava bacterial blight resistance under natural infection conditions Identificación de QTL de resistencia a la bacteriosis vascular en yuca bajo condiciones naturales de infección Soto Sedano, Johana Mora Moreno, Rubén Eduardo Calle, Fernando López Carrascal, Camilo Ernesto manihot esculenta xanthomonas disease resistance genetic markers quantitative trait loci genetic maps resistencia a la enfermedad marcadores genéticos loci de rasgos cuantitativos mapas genéticos Cassava, *Manihot esculenta* Crantz, represents the main food source for more than one billion people. Cassava's production is affected by several diseases, one of the most serious is cassava bacterial blight (CBB) caused by *Xanthomonas axonopodis* pv. *manihotis* (*Xam*). A quantitative trait loci (QTL) analysis for CBB resistance was performed under natural infection conditions, using a mapping population of 99 full-sibs genotypes highly segregant and a SNP-based high dense genetic map. The phenotypic evaluation was carried out in Puerto López, Meta, Colombia, during the rainy season in 2015. Both resistant and susceptible transgressive segregants were detected in the mapping population. Through a non-parametric interval mapping analysis, two QTL were detected, explaining 10.9 and 12.6 % of phenotypic variance of resistance to field CBB. After a bioinformatics exploration four genes were identified in the QTL intervals. This work represents a contribution to the elucidation of the molecular bases of quantitative cassava resistance to *Xam*. La yuca, *Manihot esculenta* Crantz, representa la principal fuente de alimento para cerca de 1000 millones de personas. La producción de yuca se ve afectada por diversas enfermedades, una de las más serias es la bacteriosis vascular (CBB) causada por *Xanthomonas axonopodis* pv. *manihotis* (*Xam*). En este estudio se realizó un análisis de loci de rasgos cuantitativos (QTL) para la resistencia a CBB en condiciones naturales de infección, usando una población de mapeo constituida por 99 genotipos de hermanos completos segregantes y un mapa genético altamente denso basado en SNPs. La evaluación fenotípica se llevó a cabo en Puerto López (Meta), Colombia, durante la época de lluvias durante el segundo semestre de 2015. En la población de mapeo fueron detectados individuos con una segregación transgresiva tanto resistentes como susceptibles. A través de un análisis no paramétrico de intervalo simple, se detectaron dos QTL que explican el 10,9 y el 12,6 % de la varianza fenotípica de la resistencia en campo a CBB. Mediante análisis bioinformáticos se identificaron cuatro genes candidatos presentes en los intervalos de los QTL. Este trabajo representa un esfuerzo por dilucidar los mecanismos moleculares implicados en la resistencia de yuca a CBB. 2017 2017-03-22T21:30:28Z 2017-03-22T21:30:28Z Journal Article https://hdl.handle.net/10568/80479 en Open Access Universidad Nacional de Colombia Soto Sedano, Johana; Mora Moreno, Rubén Eduardo; Calle, Fernando; López Carrascal, Camilo Ernesto. 2017. QTL identification for cassava bacterial blight resistance under natural infection conditions. Acta Biológica Colombiana 22(1): 19-26. |
| spellingShingle | manihot esculenta xanthomonas disease resistance genetic markers quantitative trait loci genetic maps resistencia a la enfermedad marcadores genéticos loci de rasgos cuantitativos mapas genéticos Soto Sedano, Johana Mora Moreno, Rubén Eduardo Calle, Fernando López Carrascal, Camilo Ernesto QTL identification for cassava bacterial blight resistance under natural infection conditions |
| title | QTL identification for cassava bacterial blight resistance under natural infection conditions |
| title_full | QTL identification for cassava bacterial blight resistance under natural infection conditions |
| title_fullStr | QTL identification for cassava bacterial blight resistance under natural infection conditions |
| title_full_unstemmed | QTL identification for cassava bacterial blight resistance under natural infection conditions |
| title_short | QTL identification for cassava bacterial blight resistance under natural infection conditions |
| title_sort | qtl identification for cassava bacterial blight resistance under natural infection conditions |
| topic | manihot esculenta xanthomonas disease resistance genetic markers quantitative trait loci genetic maps resistencia a la enfermedad marcadores genéticos loci de rasgos cuantitativos mapas genéticos |
| url | https://hdl.handle.net/10568/80479 |
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