The spatial genetic differentiation of the legume pod borer, Maruca vitrata F. (Lepidoptera: Crambidae) populations in West Africa
The legume pod borer, Maruca vitrata, is an endemic insect pest that causes significant yield loss to the cowpea crop in West Africa. The application of population genetic tools is important in the management of insect pests but such data on M. vitrata is lacking. We applied a set of six microsatell...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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Cambridge University Press
2012
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/79863 |
| _version_ | 1855529050339016704 |
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| author | Agunbiade, T.A. Coates, B.S. Kim, S.K. Forgacs, D. Margam, V.M. Murdock, L.L. Ba, Malick N. Binso-Dabire, L. Baoua, I. Ishiyaku, M. Tamo, Manuele Pittendrigh, Barry Robert |
| author_browse | Agunbiade, T.A. Ba, Malick N. Baoua, I. Binso-Dabire, L. Coates, B.S. Forgacs, D. Ishiyaku, M. Kim, S.K. Margam, V.M. Murdock, L.L. Pittendrigh, Barry Robert Tamo, Manuele |
| author_facet | Agunbiade, T.A. Coates, B.S. Kim, S.K. Forgacs, D. Margam, V.M. Murdock, L.L. Ba, Malick N. Binso-Dabire, L. Baoua, I. Ishiyaku, M. Tamo, Manuele Pittendrigh, Barry Robert |
| author_sort | Agunbiade, T.A. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The legume pod borer, Maruca vitrata, is an endemic insect pest that causes significant yield loss to the cowpea crop in West Africa. The application of population genetic tools is important in the management of insect pests but such data on M. vitrata is lacking. We applied a set of six microsatellite markers to assess the population structure of M. vitrata collected at five sites from Burkina Faso, Niger and Nigeria. Observed polymorphisms ranged from one (marker 3393) to eight (marker 32008) alleles per locus. Observed and expected heterozygosities ranged from 0.0 to 0.8 and 0.0 to 0.6, respectively. Three of the loci in samples from Nigeria and Burkina
Faso deviated significantly from Hardy-Weinberg Equilibrium (HWE), whereas no loci deviated significantly in samples from Niger. Analysis of molecular variance (AMOVA) indicated that 67.3% level of the genetic variation was within individuals compared to 17.3% among populations. A global estimate of FST=0.1 (ENA corrected FST=0.1) was significant (P≤0.05) and corroborated by pairwise FST values that were significant among all possible comparisons. A significant correlation was predicted between genetic divergence and geographic distance between subpopulations (R2=0.6, P=0.04), and cluster analysis by the program STRUCTURE predicted that co-ancestry of genotypes were indicative of three distinct populations. The spatialgenetic variance among M. vitrata in West Africa may be due to limited gene flow, south-north seasonal movement pattern or other reproductive barriers. This information is important for the cultural, chemical and biological control strategies for managing M. vitrata. |
| format | Journal Article |
| id | CGSpace79863 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2012 |
| publishDateRange | 2012 |
| publishDateSort | 2012 |
| publisher | Cambridge University Press |
| publisherStr | Cambridge University Press |
| record_format | dspace |
| spelling | CGSpace798632024-11-15T08:52:40Z The spatial genetic differentiation of the legume pod borer, Maruca vitrata F. (Lepidoptera: Crambidae) populations in West Africa Agunbiade, T.A. Coates, B.S. Kim, S.K. Forgacs, D. Margam, V.M. Murdock, L.L. Ba, Malick N. Binso-Dabire, L. Baoua, I. Ishiyaku, M. Tamo, Manuele Pittendrigh, Barry Robert microsatellites cowpea pests The legume pod borer, Maruca vitrata, is an endemic insect pest that causes significant yield loss to the cowpea crop in West Africa. The application of population genetic tools is important in the management of insect pests but such data on M. vitrata is lacking. We applied a set of six microsatellite markers to assess the population structure of M. vitrata collected at five sites from Burkina Faso, Niger and Nigeria. Observed polymorphisms ranged from one (marker 3393) to eight (marker 32008) alleles per locus. Observed and expected heterozygosities ranged from 0.0 to 0.8 and 0.0 to 0.6, respectively. Three of the loci in samples from Nigeria and Burkina Faso deviated significantly from Hardy-Weinberg Equilibrium (HWE), whereas no loci deviated significantly in samples from Niger. Analysis of molecular variance (AMOVA) indicated that 67.3% level of the genetic variation was within individuals compared to 17.3% among populations. A global estimate of FST=0.1 (ENA corrected FST=0.1) was significant (P≤0.05) and corroborated by pairwise FST values that were significant among all possible comparisons. A significant correlation was predicted between genetic divergence and geographic distance between subpopulations (R2=0.6, P=0.04), and cluster analysis by the program STRUCTURE predicted that co-ancestry of genotypes were indicative of three distinct populations. The spatialgenetic variance among M. vitrata in West Africa may be due to limited gene flow, south-north seasonal movement pattern or other reproductive barriers. This information is important for the cultural, chemical and biological control strategies for managing M. vitrata. 2012-10 2017-02-13T13:41:54Z 2017-02-13T13:41:54Z Journal Article https://hdl.handle.net/10568/79863 en Limited Access Cambridge University Press Agunbiade, T., Coates, B.S., Kim, K.S., Forgacs, D., Margam, V.M., Murdock, L.L., ... & Tamò, M. (2012). The spatial genetic differentiation of the legume pod borer, Maruca vitrata F.(Lepidoptera: Crambidae) populations in West Africa. Bulletin of Entomological Research, 102(05), 589-599. |
| spellingShingle | microsatellites cowpea pests Agunbiade, T.A. Coates, B.S. Kim, S.K. Forgacs, D. Margam, V.M. Murdock, L.L. Ba, Malick N. Binso-Dabire, L. Baoua, I. Ishiyaku, M. Tamo, Manuele Pittendrigh, Barry Robert The spatial genetic differentiation of the legume pod borer, Maruca vitrata F. (Lepidoptera: Crambidae) populations in West Africa |
| title | The spatial genetic differentiation of the legume pod borer, Maruca vitrata F. (Lepidoptera: Crambidae) populations in West Africa |
| title_full | The spatial genetic differentiation of the legume pod borer, Maruca vitrata F. (Lepidoptera: Crambidae) populations in West Africa |
| title_fullStr | The spatial genetic differentiation of the legume pod borer, Maruca vitrata F. (Lepidoptera: Crambidae) populations in West Africa |
| title_full_unstemmed | The spatial genetic differentiation of the legume pod borer, Maruca vitrata F. (Lepidoptera: Crambidae) populations in West Africa |
| title_short | The spatial genetic differentiation of the legume pod borer, Maruca vitrata F. (Lepidoptera: Crambidae) populations in West Africa |
| title_sort | spatial genetic differentiation of the legume pod borer maruca vitrata f lepidoptera crambidae populations in west africa |
| topic | microsatellites cowpea pests |
| url | https://hdl.handle.net/10568/79863 |
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