Highthroughput genomic sequencing of cassava bacterial blight strains identifies conserved effectors to target for durable resistance
Cassava bacterial blight (CBB), incited by Xanthomonas axonopodis pv. manihotis (Xam), is the most important bacterial disease of cassava, a staple food source for millions of people in developing countries. Here we present a widely applicable strategy for elucidating the virulence components of a p...
| Main Authors: | , , , , , , , , , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
National Academy of Sciences
2012
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/79848 |
| _version_ | 1855524993933246464 |
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| author | Bart, R. Cohn, M. Kassen, A. McCallum, E. Shybut, M. Petriello, A. Krasileva, K. Dahlbeck, D. Medina, C. Alicai, Titus Kumar, P. Lava Moreira, L. Neto, J. Verdier, Valerie M. Santana, M. Kositcharoenkul, N. Vanderschuren, H. Gruissem, W. Bernal, A. Staskawicz, B. |
| author_browse | Alicai, Titus Bart, R. Bernal, A. Cohn, M. Dahlbeck, D. Gruissem, W. Kassen, A. Kositcharoenkul, N. Krasileva, K. Kumar, P. Lava McCallum, E. Medina, C. Moreira, L. Neto, J. Petriello, A. Santana, M. Shybut, M. Staskawicz, B. Vanderschuren, H. Verdier, Valerie M. |
| author_facet | Bart, R. Cohn, M. Kassen, A. McCallum, E. Shybut, M. Petriello, A. Krasileva, K. Dahlbeck, D. Medina, C. Alicai, Titus Kumar, P. Lava Moreira, L. Neto, J. Verdier, Valerie M. Santana, M. Kositcharoenkul, N. Vanderschuren, H. Gruissem, W. Bernal, A. Staskawicz, B. |
| author_sort | Bart, R. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Cassava bacterial blight (CBB), incited by Xanthomonas axonopodis pv. manihotis (Xam), is the most important bacterial disease of cassava, a staple food source for millions of people in developing countries. Here we present a widely applicable strategy for elucidating the virulence components of a pathogen population. We report Illumina-based draft genomes for 65 Xam strains and deduce the phylogenetic relatedness of Xam across the areas where cassava is grown. Using an extensive database of effector proteins from animal and plant pathogens, we identify the effector repertoire for each sequenced strain and use a comparative sequence analysis to deduce the least polymorphic of the conserved effectors. These highly conserved effectors have been maintained over 11 countries, three continents, and 70 y of evolution and as such represent ideal targets for developing resistance strategies. |
| format | Journal Article |
| id | CGSpace79848 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2012 |
| publishDateRange | 2012 |
| publishDateSort | 2012 |
| publisher | National Academy of Sciences |
| publisherStr | National Academy of Sciences |
| record_format | dspace |
| spelling | CGSpace798482025-01-27T15:00:52Z Highthroughput genomic sequencing of cassava bacterial blight strains identifies conserved effectors to target for durable resistance Bart, R. Cohn, M. Kassen, A. McCallum, E. Shybut, M. Petriello, A. Krasileva, K. Dahlbeck, D. Medina, C. Alicai, Titus Kumar, P. Lava Moreira, L. Neto, J. Verdier, Valerie M. Santana, M. Kositcharoenkul, N. Vanderschuren, H. Gruissem, W. Bernal, A. Staskawicz, B. innate immunity type three effectors next-generation sequencing cassava plant diseases xanthomonas axonopodis Cassava bacterial blight (CBB), incited by Xanthomonas axonopodis pv. manihotis (Xam), is the most important bacterial disease of cassava, a staple food source for millions of people in developing countries. Here we present a widely applicable strategy for elucidating the virulence components of a pathogen population. We report Illumina-based draft genomes for 65 Xam strains and deduce the phylogenetic relatedness of Xam across the areas where cassava is grown. Using an extensive database of effector proteins from animal and plant pathogens, we identify the effector repertoire for each sequenced strain and use a comparative sequence analysis to deduce the least polymorphic of the conserved effectors. These highly conserved effectors have been maintained over 11 countries, three continents, and 70 y of evolution and as such represent ideal targets for developing resistance strategies. 2012-07-10 2017-02-13T13:41:47Z 2017-02-13T13:41:47Z Journal Article https://hdl.handle.net/10568/79848 en Open Access National Academy of Sciences Bart, R., Cohn, M., Kassen, A., McCallum, E., Shybut, M., Petriello, A., … Staskawicz, B. (2012). Highthroughput genomic sequencing of cassava bacterial blight strains identifies conserved effectors to target for durable. Resistance. In: Proceedings of the National Academy of Sciences of the United States of America, 109(28), E1972-E1979. |
| spellingShingle | innate immunity type three effectors next-generation sequencing cassava plant diseases xanthomonas axonopodis Bart, R. Cohn, M. Kassen, A. McCallum, E. Shybut, M. Petriello, A. Krasileva, K. Dahlbeck, D. Medina, C. Alicai, Titus Kumar, P. Lava Moreira, L. Neto, J. Verdier, Valerie M. Santana, M. Kositcharoenkul, N. Vanderschuren, H. Gruissem, W. Bernal, A. Staskawicz, B. Highthroughput genomic sequencing of cassava bacterial blight strains identifies conserved effectors to target for durable resistance |
| title | Highthroughput genomic sequencing of cassava bacterial blight strains identifies conserved effectors to target for durable resistance |
| title_full | Highthroughput genomic sequencing of cassava bacterial blight strains identifies conserved effectors to target for durable resistance |
| title_fullStr | Highthroughput genomic sequencing of cassava bacterial blight strains identifies conserved effectors to target for durable resistance |
| title_full_unstemmed | Highthroughput genomic sequencing of cassava bacterial blight strains identifies conserved effectors to target for durable resistance |
| title_short | Highthroughput genomic sequencing of cassava bacterial blight strains identifies conserved effectors to target for durable resistance |
| title_sort | highthroughput genomic sequencing of cassava bacterial blight strains identifies conserved effectors to target for durable resistance |
| topic | innate immunity type three effectors next-generation sequencing cassava plant diseases xanthomonas axonopodis |
| url | https://hdl.handle.net/10568/79848 |
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