Re-evaluation of Yam Mosaic Virus (YMV) detection methods
Accurate and timely detection is vital for mitigation of tuber yield losses resulting from yam mosaic virus (YMV) infection on yam, a major food security crop in West Africa. The observation, from our previous studies, that the triple antibody sandwich enzyme-linked immunosorbent assay (TAS-ELISA),...
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| Format: | Journal Article |
| Language: | Inglés |
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International Digital Organization for Scientific Information (IDOSI)
2012
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| Online Access: | https://hdl.handle.net/10568/79829 |
| _version_ | 1855539726777319424 |
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| author | Eni, A. Hughes, J. Asiedu, Robert Rey, M. |
| author_browse | Asiedu, Robert Eni, A. Hughes, J. Rey, M. |
| author_facet | Eni, A. Hughes, J. Asiedu, Robert Rey, M. |
| author_sort | Eni, A. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Accurate and timely detection is vital for mitigation of tuber yield losses resulting from yam mosaic virus (YMV) infection on yam, a major food security crop in West Africa. The observation, from our previous studies, that the triple antibody sandwich enzyme-linked immunosorbent assay (TAS-ELISA), the most commonly used detection method for YMV, detected the virus in significantly less leaf samples than immunocapture reverse transcription polymerase chain reaction (IC-RT-PCR) necessitated a re-evaluation of YMV detection methods. In the present study, eighteen previously tested YMV positive leaf samples from Benin and Ghana were re-tested using TAS-ELISA, Protein A-sandwich (PAS) ELISA and IC-RT-PCR. Three sap dilutions, 1/10, 1/50 and 1/100, were tested for each sample. Both at 1/10 and 1/50 dilutions, PAS-ELISA and IC-RT-PCR detected YMV in 11 (61.1%) and 12 (66.7%) of the leaves respectively. Virus detection by PAS-ELISA reduced to 50% at 1/100 sap dilution and increased to 77.8% in IC-RT-PCR. YMV detection by TAS-ELISA varied between 38.9% and 16.7% at 1/10 and 1/100 dilutions respectively. These results indicate a deficiency in the use of TAS-ELISA as a sole YMV certification method since the detecting monoclonal antibody used in this assay may be strain specific. The use of PAS-ELISA at a 1/10 sap dilution is suggested for YMV detection where the facilities for molecular detection are unavailable. |
| format | Journal Article |
| id | CGSpace79829 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2012 |
| publishDateRange | 2012 |
| publishDateSort | 2012 |
| publisher | International Digital Organization for Scientific Information (IDOSI) |
| publisherStr | International Digital Organization for Scientific Information (IDOSI) |
| record_format | dspace |
| spelling | CGSpace798292023-06-13T05:03:07Z Re-evaluation of Yam Mosaic Virus (YMV) detection methods Eni, A. Hughes, J. Asiedu, Robert Rey, M. yam mosaic virus detection sensitivity genus potyvirus yams dioscorea epidemiological isolation alkaline phosphatase Accurate and timely detection is vital for mitigation of tuber yield losses resulting from yam mosaic virus (YMV) infection on yam, a major food security crop in West Africa. The observation, from our previous studies, that the triple antibody sandwich enzyme-linked immunosorbent assay (TAS-ELISA), the most commonly used detection method for YMV, detected the virus in significantly less leaf samples than immunocapture reverse transcription polymerase chain reaction (IC-RT-PCR) necessitated a re-evaluation of YMV detection methods. In the present study, eighteen previously tested YMV positive leaf samples from Benin and Ghana were re-tested using TAS-ELISA, Protein A-sandwich (PAS) ELISA and IC-RT-PCR. Three sap dilutions, 1/10, 1/50 and 1/100, were tested for each sample. Both at 1/10 and 1/50 dilutions, PAS-ELISA and IC-RT-PCR detected YMV in 11 (61.1%) and 12 (66.7%) of the leaves respectively. Virus detection by PAS-ELISA reduced to 50% at 1/100 sap dilution and increased to 77.8% in IC-RT-PCR. YMV detection by TAS-ELISA varied between 38.9% and 16.7% at 1/10 and 1/100 dilutions respectively. These results indicate a deficiency in the use of TAS-ELISA as a sole YMV certification method since the detecting monoclonal antibody used in this assay may be strain specific. The use of PAS-ELISA at a 1/10 sap dilution is suggested for YMV detection where the facilities for molecular detection are unavailable. 2012 2017-02-13T13:41:36Z 2017-02-13T13:41:36Z Journal Article https://hdl.handle.net/10568/79829 en Limited Access International Digital Organization for Scientific Information (IDOSI) Eni, A., Hughes, J., Asiedu, R., & Rey, M. (2012). Re-evaluation of yam mosaic virus (YMV) detection methods. Academic Journal of Plant Sciences, 5(1), 18-22. |
| spellingShingle | yam mosaic virus detection sensitivity genus potyvirus yams dioscorea epidemiological isolation alkaline phosphatase Eni, A. Hughes, J. Asiedu, Robert Rey, M. Re-evaluation of Yam Mosaic Virus (YMV) detection methods |
| title | Re-evaluation of Yam Mosaic Virus (YMV) detection methods |
| title_full | Re-evaluation of Yam Mosaic Virus (YMV) detection methods |
| title_fullStr | Re-evaluation of Yam Mosaic Virus (YMV) detection methods |
| title_full_unstemmed | Re-evaluation of Yam Mosaic Virus (YMV) detection methods |
| title_short | Re-evaluation of Yam Mosaic Virus (YMV) detection methods |
| title_sort | re evaluation of yam mosaic virus ymv detection methods |
| topic | yam mosaic virus detection sensitivity genus potyvirus yams dioscorea epidemiological isolation alkaline phosphatase |
| url | https://hdl.handle.net/10568/79829 |
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