Complete viral genome sequence and discovery of novel viruses by deep sequencing of small RNAs: A generic method for diagnosis, discovery and sequencing of viruses
We report the first identification of novel viruses, and sequence of an entire viral genome, by a single step of high-throughput parallel sequencing of small RNAs from diseased, as well as symptomless plants. Contigs were assembled from sequenced total siRNA from plants using small sequence assembly...
| Main Authors: | , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Elsevier
2009
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/79776 |
| _version_ | 1855529951335284736 |
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| author | Kreuze, Jan F. Pérez, A. Untiveros, M. Quispe, D. Fuentes, S. Barker, I. Simon, R. |
| author_browse | Barker, I. Fuentes, S. Kreuze, Jan F. Pérez, A. Quispe, D. Simon, R. Untiveros, M. |
| author_facet | Kreuze, Jan F. Pérez, A. Untiveros, M. Quispe, D. Fuentes, S. Barker, I. Simon, R. |
| author_sort | Kreuze, Jan F. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | We report the first identification of novel viruses, and sequence of an entire viral genome, by a single step of high-throughput parallel sequencing of small RNAs from diseased, as well as symptomless plants. Contigs were assembled from sequenced total siRNA from plants using small sequence assembly software and could positively identify RNA, ssDNA and dsDNA reverse transcribing viruses and in one case spanned the entire genome. The results present a novel approach which cannot only identify known viral pathogens, occurring at extremely low titers, but also novel viruses, without the necessity of any prior knowledge. |
| format | Journal Article |
| id | CGSpace79776 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2009 |
| publishDateRange | 2009 |
| publishDateSort | 2009 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | CGSpace797762025-04-17T08:26:30Z Complete viral genome sequence and discovery of novel viruses by deep sequencing of small RNAs: A generic method for diagnosis, discovery and sequencing of viruses Kreuze, Jan F. Pérez, A. Untiveros, M. Quispe, D. Fuentes, S. Barker, I. Simon, R. genomes viroses diagnosis plant viruses sweet potatoes We report the first identification of novel viruses, and sequence of an entire viral genome, by a single step of high-throughput parallel sequencing of small RNAs from diseased, as well as symptomless plants. Contigs were assembled from sequenced total siRNA from plants using small sequence assembly software and could positively identify RNA, ssDNA and dsDNA reverse transcribing viruses and in one case spanned the entire genome. The results present a novel approach which cannot only identify known viral pathogens, occurring at extremely low titers, but also novel viruses, without the necessity of any prior knowledge. 2009-05 2017-02-07T13:03:33Z 2017-02-07T13:03:33Z Journal Article https://hdl.handle.net/10568/79776 en Limited Access Elsevier Kreuze, J.F.; Perez, A.; Untiveros, M.; Quispe, D.; Fuentes, S.; Barker, I.; Simon, R. 2009. Complete viral genome sequence and discovery of novel viruses by deep sequencing of small RNAs: A generic method for diagnosis, discovery and sequencing of viruses. Virology. (USA). ISSN 0042-6822. 388(1):1-7. |
| spellingShingle | genomes viroses diagnosis plant viruses sweet potatoes Kreuze, Jan F. Pérez, A. Untiveros, M. Quispe, D. Fuentes, S. Barker, I. Simon, R. Complete viral genome sequence and discovery of novel viruses by deep sequencing of small RNAs: A generic method for diagnosis, discovery and sequencing of viruses |
| title | Complete viral genome sequence and discovery of novel viruses by deep sequencing of small RNAs: A generic method for diagnosis, discovery and sequencing of viruses |
| title_full | Complete viral genome sequence and discovery of novel viruses by deep sequencing of small RNAs: A generic method for diagnosis, discovery and sequencing of viruses |
| title_fullStr | Complete viral genome sequence and discovery of novel viruses by deep sequencing of small RNAs: A generic method for diagnosis, discovery and sequencing of viruses |
| title_full_unstemmed | Complete viral genome sequence and discovery of novel viruses by deep sequencing of small RNAs: A generic method for diagnosis, discovery and sequencing of viruses |
| title_short | Complete viral genome sequence and discovery of novel viruses by deep sequencing of small RNAs: A generic method for diagnosis, discovery and sequencing of viruses |
| title_sort | complete viral genome sequence and discovery of novel viruses by deep sequencing of small rnas a generic method for diagnosis discovery and sequencing of viruses |
| topic | genomes viroses diagnosis plant viruses sweet potatoes |
| url | https://hdl.handle.net/10568/79776 |
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