Phenotyping Brachiaria genotypes to assess Rhizoctonia resistance by comparing three inoculum types
Rhizoctonia foliar blight, caused by Rhizoctonia solani, is an important disease of Brachiaria spp. in tropical America. Host-plant resistance is an attractive option for disease management. In this study, we evaluated three inoculum types (mycelium-infected agar disc, microdiscs suspensions, and mi...
| Autores principales: | , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
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Scientific Societies
2017
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| Acceso en línea: | https://hdl.handle.net/10568/79747 |
| _version_ | 1855543452211609600 |
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| author | Hernández, Luis Miguel Sotelo, Guillermo Bonilla, Ximena P. Álvarez, Elizabeth Miles, John W. Worthington, Margaret |
| author_browse | Bonilla, Ximena P. Hernández, Luis Miguel Miles, John W. Sotelo, Guillermo Worthington, Margaret Álvarez, Elizabeth |
| author_facet | Hernández, Luis Miguel Sotelo, Guillermo Bonilla, Ximena P. Álvarez, Elizabeth Miles, John W. Worthington, Margaret |
| author_sort | Hernández, Luis Miguel |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Rhizoctonia foliar blight, caused by Rhizoctonia solani, is an important disease of Brachiaria spp. in tropical America. Host-plant resistance is an attractive option for disease management. In this study, we evaluated three inoculum types (mycelium-infected agar disc, microdiscs suspensions, and microencapsulated-mycelium suspensions) in order to identify a rapid and accurate method for mass screening of Brachiaria genotypes for resistance to Rhizoctonia spp. in greenhouse trials. Visual damage score, area under the disease progress curve, and percent chlorophyll loss were estimated to determine the most accurate and precise method for evaluating Rhizoctonia resistance. The microencapsulated-mycelium solution (0.75 g/ml in potato dextrose broth sprayed on plants 30 days after planting) caused greater foliar damage than the other inoculum types and allowed effective discrimination between resistant and susceptible genotypes. The effectiveness of spray-applied, microencapsulated-mycelium was further corroborated by the evaluation of 350 genotypes not previously selected for resistance to Rhizoctonia spp., which varied significantly in their reaction to R. solani. The microencapsulated-mycelium methodology has several advantages over existing methods, including its high-throughput capacity, efficient use of time and space, ease of quantification of inoculum, and consistent results over replicate trials. This methodology could be applied to assess resistance to Rhizoctonia spp. in other crops. |
| format | Journal Article |
| id | CGSpace79747 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| publisher | Scientific Societies |
| publisherStr | Scientific Societies |
| record_format | dspace |
| spelling | CGSpace797472025-03-13T09:46:01Z Phenotyping Brachiaria genotypes to assess Rhizoctonia resistance by comparing three inoculum types Hernández, Luis Miguel Sotelo, Guillermo Bonilla, Ximena P. Álvarez, Elizabeth Miles, John W. Worthington, Margaret feed crops rhizoctonia solani host plant resistance brachiaria genotypes plant diseases plan breeding disease control enfermedades de las plantas control de plagas control de enfermedades fitomejoramiento Rhizoctonia foliar blight, caused by Rhizoctonia solani, is an important disease of Brachiaria spp. in tropical America. Host-plant resistance is an attractive option for disease management. In this study, we evaluated three inoculum types (mycelium-infected agar disc, microdiscs suspensions, and microencapsulated-mycelium suspensions) in order to identify a rapid and accurate method for mass screening of Brachiaria genotypes for resistance to Rhizoctonia spp. in greenhouse trials. Visual damage score, area under the disease progress curve, and percent chlorophyll loss were estimated to determine the most accurate and precise method for evaluating Rhizoctonia resistance. The microencapsulated-mycelium solution (0.75 g/ml in potato dextrose broth sprayed on plants 30 days after planting) caused greater foliar damage than the other inoculum types and allowed effective discrimination between resistant and susceptible genotypes. The effectiveness of spray-applied, microencapsulated-mycelium was further corroborated by the evaluation of 350 genotypes not previously selected for resistance to Rhizoctonia spp., which varied significantly in their reaction to R. solani. The microencapsulated-mycelium methodology has several advantages over existing methods, including its high-throughput capacity, efficient use of time and space, ease of quantification of inoculum, and consistent results over replicate trials. This methodology could be applied to assess resistance to Rhizoctonia spp. in other crops. 2017-06 2017-02-03T16:56:35Z 2017-02-03T16:56:35Z Journal Article https://hdl.handle.net/10568/79747 en Open Access Scientific Societies Hernandez, Luis Miguel; Sotelo, Guillermo; Bonilla, Ximena ; Álvarez, Elizabeth; Miles, John; Worthington, Margaret. 2017. Phenotyping Brachiaria genotypes to assess Rhizoctonia resistance by comparing three inoculum types. Plant Disease, 101(6): 916-923. |
| spellingShingle | feed crops rhizoctonia solani host plant resistance brachiaria genotypes plant diseases plan breeding disease control enfermedades de las plantas control de plagas control de enfermedades fitomejoramiento Hernández, Luis Miguel Sotelo, Guillermo Bonilla, Ximena P. Álvarez, Elizabeth Miles, John W. Worthington, Margaret Phenotyping Brachiaria genotypes to assess Rhizoctonia resistance by comparing three inoculum types |
| title | Phenotyping Brachiaria genotypes to assess Rhizoctonia resistance by comparing three inoculum types |
| title_full | Phenotyping Brachiaria genotypes to assess Rhizoctonia resistance by comparing three inoculum types |
| title_fullStr | Phenotyping Brachiaria genotypes to assess Rhizoctonia resistance by comparing three inoculum types |
| title_full_unstemmed | Phenotyping Brachiaria genotypes to assess Rhizoctonia resistance by comparing three inoculum types |
| title_short | Phenotyping Brachiaria genotypes to assess Rhizoctonia resistance by comparing three inoculum types |
| title_sort | phenotyping brachiaria genotypes to assess rhizoctonia resistance by comparing three inoculum types |
| topic | feed crops rhizoctonia solani host plant resistance brachiaria genotypes plant diseases plan breeding disease control enfermedades de las plantas control de plagas control de enfermedades fitomejoramiento |
| url | https://hdl.handle.net/10568/79747 |
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