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Studies on in Vitrro assessment of "drug resistance" in trypanosoma vivax.

Six stocks of Trypanosoma vivax were investigated for their drug sensitivity to isometamidium chloride and diminazene aceturate. It was evaluated whether in vitro tests had the potential to distinguish between drug-sensitive and drug-resistant stocks of T vivax. Four in vitro tests were employed in...

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Main Author: Kitosi, D.S.
Format: Tesis
Language:Inglés
Published: University of Nairobi 1990
Subjects:
cattle
mice
blood cells
infection
Online Access:https://hdl.handle.net/10568/79674
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author Kitosi, D.S.
author_browse Kitosi, D.S.
author_facet Kitosi, D.S.
author_sort Kitosi, D.S.
collection Repository of Agricultural Research Outputs (CGSpace)
description Six stocks of Trypanosoma vivax were investigated for their drug sensitivity to isometamidium chloride and diminazene aceturate. It was evaluated whether in vitro tests had the potential to distinguish between drug-sensitive and drug-resistant stocks of T vivax. Four in vitro tests were employed in drug sensitivity assessment: (1) Long Term Viability Assay (20 days) with insect forms at 27 C, (2) 3H-Hypoxanthine Incorporation Test (48 hrs) with bloodstream forms at 340C, (3) Drug Incubation Infectivity Test (DIIT) with bloodstream forms at 370C and (4) Drug Incubation Survival Test with bloodstream forms at 340C. All tests were able to show an antitrypanosomal effect of both, isometamidium chloride and diminazene aceturate on T vivax in vitro. The antitrypanosomal effects were detected by rounding and death of epimastigotes, inhibition of 3H-Hypoxanthine incorporation by bloodstream forms, loss of infectivity to mice and reduction in the number of survivors. The Long Term Viability Assay and the Drug Incubation Survival Test showed potential to distinguish between isometamidium chloride-sensitive and isometamidium chloride-resistant stocks or clones . of T vivax. Results on drug sensitivity of T vivax stocks obtained with these tests correlated with results in cattle or mice. A correlation between in vitro and in vivo results was not observed regarding diminazene aceturate. When cattle were infected with in vitro produced metacyclics, resistance of bloodstream forms of T vivax stock CP 2171 was retained to treatment with 0.5 and 1.0 mg/kg isometamidium chloride after continuous in vitro propagation of epimastigotes for 30 months. The reduced sensitivity of epimastigotes of CP 2171 to isometamidium in vitro was similar in cultures initiated from these cattle compared with results obtained in cultures initiated from cattle infected with the original stabilate.
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publishDate 1990
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spelling CGSpace796742023-02-15T11:19:21Z Studies on in Vitrro assessment of "drug resistance" in trypanosoma vivax. Kitosi, D.S. cattle mice blood cells infection Six stocks of Trypanosoma vivax were investigated for their drug sensitivity to isometamidium chloride and diminazene aceturate. It was evaluated whether in vitro tests had the potential to distinguish between drug-sensitive and drug-resistant stocks of T vivax. Four in vitro tests were employed in drug sensitivity assessment: (1) Long Term Viability Assay (20 days) with insect forms at 27 C, (2) 3H-Hypoxanthine Incorporation Test (48 hrs) with bloodstream forms at 340C, (3) Drug Incubation Infectivity Test (DIIT) with bloodstream forms at 370C and (4) Drug Incubation Survival Test with bloodstream forms at 340C. All tests were able to show an antitrypanosomal effect of both, isometamidium chloride and diminazene aceturate on T vivax in vitro. The antitrypanosomal effects were detected by rounding and death of epimastigotes, inhibition of 3H-Hypoxanthine incorporation by bloodstream forms, loss of infectivity to mice and reduction in the number of survivors. The Long Term Viability Assay and the Drug Incubation Survival Test showed potential to distinguish between isometamidium chloride-sensitive and isometamidium chloride-resistant stocks or clones . of T vivax. Results on drug sensitivity of T vivax stocks obtained with these tests correlated with results in cattle or mice. A correlation between in vitro and in vivo results was not observed regarding diminazene aceturate. When cattle were infected with in vitro produced metacyclics, resistance of bloodstream forms of T vivax stock CP 2171 was retained to treatment with 0.5 and 1.0 mg/kg isometamidium chloride after continuous in vitro propagation of epimastigotes for 30 months. The reduced sensitivity of epimastigotes of CP 2171 to isometamidium in vitro was similar in cultures initiated from these cattle compared with results obtained in cultures initiated from cattle infected with the original stabilate. 1990 2017-02-03T11:04:28Z 2017-02-03T11:04:28Z Thesis https://hdl.handle.net/10568/79674 en Limited Access University of Nairobi Kitosi, D. S. 1990. Studies on in Vitrro assessment of "drug resistance" in trypanosoma vivax. MSc thesis in Veterinary Pathology and Microbiology.
spellingShingle cattle
mice
blood cells
infection
Kitosi, D.S.
Studies on in Vitrro assessment of "drug resistance" in trypanosoma vivax.
title Studies on in Vitrro assessment of "drug resistance" in trypanosoma vivax.
title_full Studies on in Vitrro assessment of "drug resistance" in trypanosoma vivax.
title_fullStr Studies on in Vitrro assessment of "drug resistance" in trypanosoma vivax.
title_full_unstemmed Studies on in Vitrro assessment of "drug resistance" in trypanosoma vivax.
title_short Studies on in Vitrro assessment of "drug resistance" in trypanosoma vivax.
title_sort studies on in vitrro assessment of drug resistance in trypanosoma vivax
topic cattle
mice
blood cells
infection
url https://hdl.handle.net/10568/79674
work_keys_str_mv AT kitosids studiesoninvitrroassessmentofdrugresistanceintrypanosomavivax

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