Evaluation of T cell recognition of theileria parva homologs of apicomplexan antigens.
East Coast fever is a severe lymphoproliferative disease of cattle caused by the intracellular protozoan Theileriaparva from the family Apicomplexa, CD8+ cytotoxic T lymphocyte (CTL) mediated destruction of parasite infected lymphocytes constitutes the dominant protective immune response following e...
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| Formato: | Tesis |
| Lenguaje: | Inglés |
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University of Nairobi
2007
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| Acceso en línea: | https://hdl.handle.net/10568/79663 |
| _version_ | 1855523206065029120 |
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| author | Kangethe, R.T. |
| author_browse | Kangethe, R.T. |
| author_facet | Kangethe, R.T. |
| author_sort | Kangethe, R.T. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | East Coast fever is a severe lymphoproliferative disease of cattle caused by the intracellular protozoan Theileriaparva from the family Apicomplexa, CD8+ cytotoxic T lymphocyte (CTL) mediated destruction of parasite infected lymphocytes constitutes the dominant protective immune response following exposure to infection or vaccination using a live infection and treatment protocol. Appropriate delivery of parasite antigens targeted by CTL from T parva immune cattle has long been proposed as a strategy for the development of a subunit vaccine that would contribute substantially to a sustainable integrated ECF control program. Gene homologs encoding antigens from other apicomplexan parasites constitute a source of vaccine candidate antigens. The aim of this study was to evaluate the recognition of T parva homologs of known apicomplexan antigens by CD8+ and CD4+ T lymphocytes isolated from cattle immunized by a live vaccine. Eight T parva homo logs of apicomplexan antigens were sub-cloned into a eukaryotic expression vector and transiently transfected into bovine antigen presenting cells (APC). Recognition of transfected APC by T parva specific CD8+ CTL lines was assessed using an IFN-y ELISpot assay but none of the 7 CTL lines tested recognized any of the homologs. A recombinant protein was generated representing atruncated version of one T parva protein designated X9-3 (a homolog of T Complex Protein-I zeta subunit in Babesia microti) and tested in immunoassays for recognition by T cells from immune cattle. Peripheral blood mononuclear cells from 3112cattle and a T parva specific polyclonal CD4+ T cell line from immune cattle BW014 responded to the X9-3 protein. The results suggest that none of the eight homolog genes are CTL target antigens but X9-3 is a candidate T-helper cell target antigen and should be considered for inclusion in a subunit vaccine against ECF |
| format | Tesis |
| id | CGSpace79663 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2007 |
| publishDateRange | 2007 |
| publishDateSort | 2007 |
| publisher | University of Nairobi |
| publisherStr | University of Nairobi |
| record_format | dspace |
| spelling | CGSpace796632023-02-15T11:15:19Z Evaluation of T cell recognition of theileria parva homologs of apicomplexan antigens. Kangethe, R.T. theileria antigens cells East Coast fever is a severe lymphoproliferative disease of cattle caused by the intracellular protozoan Theileriaparva from the family Apicomplexa, CD8+ cytotoxic T lymphocyte (CTL) mediated destruction of parasite infected lymphocytes constitutes the dominant protective immune response following exposure to infection or vaccination using a live infection and treatment protocol. Appropriate delivery of parasite antigens targeted by CTL from T parva immune cattle has long been proposed as a strategy for the development of a subunit vaccine that would contribute substantially to a sustainable integrated ECF control program. Gene homologs encoding antigens from other apicomplexan parasites constitute a source of vaccine candidate antigens. The aim of this study was to evaluate the recognition of T parva homologs of known apicomplexan antigens by CD8+ and CD4+ T lymphocytes isolated from cattle immunized by a live vaccine. Eight T parva homo logs of apicomplexan antigens were sub-cloned into a eukaryotic expression vector and transiently transfected into bovine antigen presenting cells (APC). Recognition of transfected APC by T parva specific CD8+ CTL lines was assessed using an IFN-y ELISpot assay but none of the 7 CTL lines tested recognized any of the homologs. A recombinant protein was generated representing atruncated version of one T parva protein designated X9-3 (a homolog of T Complex Protein-I zeta subunit in Babesia microti) and tested in immunoassays for recognition by T cells from immune cattle. Peripheral blood mononuclear cells from 3112cattle and a T parva specific polyclonal CD4+ T cell line from immune cattle BW014 responded to the X9-3 protein. The results suggest that none of the eight homolog genes are CTL target antigens but X9-3 is a candidate T-helper cell target antigen and should be considered for inclusion in a subunit vaccine against ECF 2007 2017-02-03T11:04:24Z 2017-02-03T11:04:24Z Thesis https://hdl.handle.net/10568/79663 en Limited Access University of Nairobi Kangethe, R. T. 2007. Evaluation of T cell recognition of theileria parva homologs of apicomplexan antigens. MSc thesis in Biochemistry. University of Nairobi. |
| spellingShingle | theileria antigens cells Kangethe, R.T. Evaluation of T cell recognition of theileria parva homologs of apicomplexan antigens. |
| title | Evaluation of T cell recognition of theileria parva homologs of apicomplexan antigens. |
| title_full | Evaluation of T cell recognition of theileria parva homologs of apicomplexan antigens. |
| title_fullStr | Evaluation of T cell recognition of theileria parva homologs of apicomplexan antigens. |
| title_full_unstemmed | Evaluation of T cell recognition of theileria parva homologs of apicomplexan antigens. |
| title_short | Evaluation of T cell recognition of theileria parva homologs of apicomplexan antigens. |
| title_sort | evaluation of t cell recognition of theileria parva homologs of apicomplexan antigens |
| topic | theileria antigens cells |
| url | https://hdl.handle.net/10568/79663 |
| work_keys_str_mv | AT kangethert evaluationoftcellrecognitionoftheileriaparvahomologsofapicomplexanantigens |