Characterization of casein kinase II of Trypanosoma (nannomonas) congolense.
Casein kinase II from Trypanosoma congolense has been partially purified by chromatography on DEAE-cellulose and heparin-agarose and this subunit exhibits protein kinase activity with casein as the substrate. The enzyme adhered to both the DEAE-cellulose and heparin-agarose resins and was eluted at...
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| Formato: | Tesis |
| Lenguaje: | Inglés |
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University of Nairobi
1993
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| Acceso en línea: | https://hdl.handle.net/10568/79658 |
| _version_ | 1855527566079688704 |
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| author | Janoo, R.T.K. |
| author_browse | Janoo, R.T.K. |
| author_facet | Janoo, R.T.K. |
| author_sort | Janoo, R.T.K. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Casein kinase II from Trypanosoma congolense has been partially purified by chromatography on DEAE-cellulose and heparin-agarose and this subunit exhibits protein kinase activity with casein as the substrate. The enzyme adhered to both the DEAE-cellulose and heparin-agarose resins and was eluted at low salt concentration. Sequences of predicted amino acid domains of casein kinase II, which were conserved amongst sequences from a variety of species, were employed to construct degenerate oligomers. The oligomers were used in the polymerase chain reaction from which a 399 bp PCR product was obtained and sequenced. Using the PCR product to probe northern blots revealed a single transcript of 1.55 kb. This PCR product was also employed to screen an EMBL 3A subgenomic DNA library from the bloodstream form of Trypanosoma (Nannomonas) congolense. A clone containing a 1.2 kb sequence coding for a predicted protein of 400. amino acid residues was isolated. A partial sequence of this clone, encoding g,reater than 50% of the N-terminal part of the catalytic subunit was obtained. The deduced T congolense CK II catalytic subunit sequence reveals a high Degree of identity with the casein kinase II a and at sequence from both eukaryotes and plants: This sequence of T congolense.Cs; II a subunit displays a 69.1 % homology with the Zea mays CK II a subunit and a 66.8 % identity with the Caenorhabditis elegans a subunit chain. In addition, the T congolense catalytic subunit sequence revealed a 30 % homology with jhe human serine/threonine protein kinases. Comparison of the T congolense sequence with higher eukaryotic a and at sequences reveal that it is most identical with the a subunit. |
| format | Tesis |
| id | CGSpace79658 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 1993 |
| publishDateRange | 1993 |
| publishDateSort | 1993 |
| publisher | University of Nairobi |
| publisherStr | University of Nairobi |
| record_format | dspace |
| spelling | CGSpace796582023-02-15T11:16:15Z Characterization of casein kinase II of Trypanosoma (nannomonas) congolense. Janoo, R.T.K. trypanosoma tsetse flies animal diseases Casein kinase II from Trypanosoma congolense has been partially purified by chromatography on DEAE-cellulose and heparin-agarose and this subunit exhibits protein kinase activity with casein as the substrate. The enzyme adhered to both the DEAE-cellulose and heparin-agarose resins and was eluted at low salt concentration. Sequences of predicted amino acid domains of casein kinase II, which were conserved amongst sequences from a variety of species, were employed to construct degenerate oligomers. The oligomers were used in the polymerase chain reaction from which a 399 bp PCR product was obtained and sequenced. Using the PCR product to probe northern blots revealed a single transcript of 1.55 kb. This PCR product was also employed to screen an EMBL 3A subgenomic DNA library from the bloodstream form of Trypanosoma (Nannomonas) congolense. A clone containing a 1.2 kb sequence coding for a predicted protein of 400. amino acid residues was isolated. A partial sequence of this clone, encoding g,reater than 50% of the N-terminal part of the catalytic subunit was obtained. The deduced T congolense CK II catalytic subunit sequence reveals a high Degree of identity with the casein kinase II a and at sequence from both eukaryotes and plants: This sequence of T congolense.Cs; II a subunit displays a 69.1 % homology with the Zea mays CK II a subunit and a 66.8 % identity with the Caenorhabditis elegans a subunit chain. In addition, the T congolense catalytic subunit sequence revealed a 30 % homology with jhe human serine/threonine protein kinases. Comparison of the T congolense sequence with higher eukaryotic a and at sequences reveal that it is most identical with the a subunit. 1993 2017-02-03T11:04:21Z 2017-02-03T11:04:21Z Thesis https://hdl.handle.net/10568/79658 en Limited Access University of Nairobi Janoo. R. T. K. 1993. Characterization of casein kinase II of Trypanosoma (nannomonas) congolense. MSc thesis. University of Nairobi. |
| spellingShingle | trypanosoma tsetse flies animal diseases Janoo, R.T.K. Characterization of casein kinase II of Trypanosoma (nannomonas) congolense. |
| title | Characterization of casein kinase II of Trypanosoma (nannomonas) congolense. |
| title_full | Characterization of casein kinase II of Trypanosoma (nannomonas) congolense. |
| title_fullStr | Characterization of casein kinase II of Trypanosoma (nannomonas) congolense. |
| title_full_unstemmed | Characterization of casein kinase II of Trypanosoma (nannomonas) congolense. |
| title_short | Characterization of casein kinase II of Trypanosoma (nannomonas) congolense. |
| title_sort | characterization of casein kinase ii of trypanosoma nannomonas congolense |
| topic | trypanosoma tsetse flies animal diseases |
| url | https://hdl.handle.net/10568/79658 |
| work_keys_str_mv | AT janoortk characterizationofcaseinkinaseiioftrypanosomanannomonascongolense |