Fine mapping of trypanosomosis resistance loci in murine by haplotype approach.
Mapping of trypanotolerance QTL in two F2 resource populations generated by crossing BALB/c and A/J with C57BLl6J has previously been achieved by Kemp et al., (1997). The subsequent fine mapping using the advanced intercross line (AIL) found the same to be true in F6 using same strains of mice (Iraq...
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| Formato: | Tesis |
| Lenguaje: | Inglés |
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University of Nairobi
2006
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| Acceso en línea: | https://hdl.handle.net/10568/79643 |
| _version_ | 1855528153265471488 |
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| author | Kamau, J.M. |
| author_browse | Kamau, J.M. |
| author_facet | Kamau, J.M. |
| author_sort | Kamau, J.M. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Mapping of trypanotolerance QTL in two F2 resource populations generated by crossing BALB/c and A/J with C57BLl6J has previously been achieved by Kemp et al., (1997). The subsequent fine mapping using the advanced intercross line (AIL) found the same to be true in F6 using same strains of mice (Iraqi et al., 2000). To confirm and fine map the QTL, a genome-wide scan for quantitative trait loci (QTLs) affecting trypanotolerance in a population of 300 F2 progeny derived from a cross between 129/J and, C57BLl6J. Two trypanotolerance QTLs (Tirl and Tir3) were detected at the genome-wide 5% or less level. Tirl and Tir3, located on Chromosomes (Chrs) 17and 1, respectively, had main effects on trypanotolerance. However, Tir2 on Chr 5 was not detected at the same level and had LOD score bellow 2. Resistant (tolerant) alleles derived from C57BLl6J were responsible for the high trypanotolerance effect. These results indicated that the genetic control of trypanotolerance is complex and the identified QTLs may provide new insights into the pathogenesis of trypanotolerance in mice as well as in livestock and humans. |
| format | Tesis |
| id | CGSpace79643 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2006 |
| publishDateRange | 2006 |
| publishDateSort | 2006 |
| publisher | University of Nairobi |
| publisherStr | University of Nairobi |
| record_format | dspace |
| spelling | CGSpace796432023-02-15T11:16:21Z Fine mapping of trypanosomosis resistance loci in murine by haplotype approach. Kamau, J.M. trypanosome loci genomes Mapping of trypanotolerance QTL in two F2 resource populations generated by crossing BALB/c and A/J with C57BLl6J has previously been achieved by Kemp et al., (1997). The subsequent fine mapping using the advanced intercross line (AIL) found the same to be true in F6 using same strains of mice (Iraqi et al., 2000). To confirm and fine map the QTL, a genome-wide scan for quantitative trait loci (QTLs) affecting trypanotolerance in a population of 300 F2 progeny derived from a cross between 129/J and, C57BLl6J. Two trypanotolerance QTLs (Tirl and Tir3) were detected at the genome-wide 5% or less level. Tirl and Tir3, located on Chromosomes (Chrs) 17and 1, respectively, had main effects on trypanotolerance. However, Tir2 on Chr 5 was not detected at the same level and had LOD score bellow 2. Resistant (tolerant) alleles derived from C57BLl6J were responsible for the high trypanotolerance effect. These results indicated that the genetic control of trypanotolerance is complex and the identified QTLs may provide new insights into the pathogenesis of trypanotolerance in mice as well as in livestock and humans. 2006 2017-02-03T11:04:14Z 2017-02-03T11:04:14Z Thesis https://hdl.handle.net/10568/79643 en Limited Access University of Nairobi Kamau, J. M. 2006. Fine mapping of trypanosomosis resistance loci in murine by haplotype approach. MSc thesis in Animal Science, University of Nairobi. |
| spellingShingle | trypanosome loci genomes Kamau, J.M. Fine mapping of trypanosomosis resistance loci in murine by haplotype approach. |
| title | Fine mapping of trypanosomosis resistance loci in murine by haplotype approach. |
| title_full | Fine mapping of trypanosomosis resistance loci in murine by haplotype approach. |
| title_fullStr | Fine mapping of trypanosomosis resistance loci in murine by haplotype approach. |
| title_full_unstemmed | Fine mapping of trypanosomosis resistance loci in murine by haplotype approach. |
| title_short | Fine mapping of trypanosomosis resistance loci in murine by haplotype approach. |
| title_sort | fine mapping of trypanosomosis resistance loci in murine by haplotype approach |
| topic | trypanosome loci genomes |
| url | https://hdl.handle.net/10568/79643 |
| work_keys_str_mv | AT kamaujm finemappingoftrypanosomosisresistancelociinmurinebyhaplotypeapproach |