Identification of Bola Class I molecules restricting cytotoxic T lymphocyte responses to a theileria parva vaccine candidate.
Theileria parva is an intracellular protozoan parasite that infects and transforms bovine lymphocytes, causing a severe Iymphoproliferative disease, East Coast fever (ECF). A robust immunity against ECF acquired after recovery is thought to be conferred by cytotoxic T lymphocytes (CTLs) specific to...
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| Format: | Tesis |
| Language: | Inglés |
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Jomo Kenyatta University of Agriculture and Technology
2005
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| Online Access: | https://hdl.handle.net/10568/79532 |
| _version_ | 1855532271085289472 |
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| author | Mwakubambanya, R.S. |
| author_browse | Mwakubambanya, R.S. |
| author_facet | Mwakubambanya, R.S. |
| author_sort | Mwakubambanya, R.S. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Theileria parva is an intracellular protozoan parasite that infects and transforms bovine lymphocytes, causing a severe Iymphoproliferative disease, East Coast fever (ECF). A robust immunity against ECF acquired after recovery is thought to be conferred by cytotoxic T lymphocytes (CTLs) specific to schizontinfected lymphocytes. Efforts are underway to develop a subunit vaccine based upon CTL target schizont antigens and to date eight CTL vaccine target antigens have been identified for evaluation. MHC class I genes encode cell surface glycoproteins that bind and present antigenic peptides to CTLs. The evaluation of these candidate vaccine antigens is to be carried out in a number of cattle of diverse BoLA class I genotypes. In this antigen evaluation process, knowledge of the BoLA class I genes restricting the CTL responses to schizont antigen is important to the development of a subunit vaccine against T. parva. However, a rapid test to define and identify BoLA class I phenotypes is lacking. The aim of this study was to identify BoLA class I genes that restrict CTL epitopes on Tp2, one of the candidate vaccine antigens and contribute to the development of a rapid PCR-based BoLA typing test. BoLA class I cDNAs were generated fromcattle immune to T. parva from which Tp2-specific CTL had been generated. BoLA class I cDNAs were co-transfected with Tp2 DNA into COS-7 cells and recognition by Tp2 specific CTL was assessed by IFN-y Enzyme Linked Immunospot and cytotoxicity assays. Two potentially novel BoLA class I genes restricting two different CTL epitopes on Tp2 sequence were identified and termed BoLA T2a and T2b. These BoLA class I cDNAs are now being used to develop PCR-based tests to identify cattle to be used in the evaluation of the vaccine potential of Tp2. |
| format | Tesis |
| id | CGSpace79532 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2005 |
| publishDateRange | 2005 |
| publishDateSort | 2005 |
| publisher | Jomo Kenyatta University of Agriculture and Technology |
| publisherStr | Jomo Kenyatta University of Agriculture and Technology |
| record_format | dspace |
| spelling | CGSpace795322023-02-15T11:17:12Z Identification of Bola Class I molecules restricting cytotoxic T lymphocyte responses to a theileria parva vaccine candidate. Mwakubambanya, R.S. vaccines theileria parva Theileria parva is an intracellular protozoan parasite that infects and transforms bovine lymphocytes, causing a severe Iymphoproliferative disease, East Coast fever (ECF). A robust immunity against ECF acquired after recovery is thought to be conferred by cytotoxic T lymphocytes (CTLs) specific to schizontinfected lymphocytes. Efforts are underway to develop a subunit vaccine based upon CTL target schizont antigens and to date eight CTL vaccine target antigens have been identified for evaluation. MHC class I genes encode cell surface glycoproteins that bind and present antigenic peptides to CTLs. The evaluation of these candidate vaccine antigens is to be carried out in a number of cattle of diverse BoLA class I genotypes. In this antigen evaluation process, knowledge of the BoLA class I genes restricting the CTL responses to schizont antigen is important to the development of a subunit vaccine against T. parva. However, a rapid test to define and identify BoLA class I phenotypes is lacking. The aim of this study was to identify BoLA class I genes that restrict CTL epitopes on Tp2, one of the candidate vaccine antigens and contribute to the development of a rapid PCR-based BoLA typing test. BoLA class I cDNAs were generated fromcattle immune to T. parva from which Tp2-specific CTL had been generated. BoLA class I cDNAs were co-transfected with Tp2 DNA into COS-7 cells and recognition by Tp2 specific CTL was assessed by IFN-y Enzyme Linked Immunospot and cytotoxicity assays. Two potentially novel BoLA class I genes restricting two different CTL epitopes on Tp2 sequence were identified and termed BoLA T2a and T2b. These BoLA class I cDNAs are now being used to develop PCR-based tests to identify cattle to be used in the evaluation of the vaccine potential of Tp2. 2005 2017-02-03T11:03:19Z 2017-02-03T11:03:19Z Thesis https://hdl.handle.net/10568/79532 en Limited Access Jomo Kenyatta University of Agriculture and Technology Mwakubambanya, R, S. 2005. Identification of Bola Class I molecules restricting cytotoxic T lymphocyte responses to a theileria parva vaccine candidate. MSc thesisin Biochemistry. Jomo Kenyatta university of Agriculture and Technology. |
| spellingShingle | vaccines theileria parva Mwakubambanya, R.S. Identification of Bola Class I molecules restricting cytotoxic T lymphocyte responses to a theileria parva vaccine candidate. |
| title | Identification of Bola Class I molecules restricting cytotoxic T lymphocyte responses to a theileria parva vaccine candidate. |
| title_full | Identification of Bola Class I molecules restricting cytotoxic T lymphocyte responses to a theileria parva vaccine candidate. |
| title_fullStr | Identification of Bola Class I molecules restricting cytotoxic T lymphocyte responses to a theileria parva vaccine candidate. |
| title_full_unstemmed | Identification of Bola Class I molecules restricting cytotoxic T lymphocyte responses to a theileria parva vaccine candidate. |
| title_short | Identification of Bola Class I molecules restricting cytotoxic T lymphocyte responses to a theileria parva vaccine candidate. |
| title_sort | identification of bola class i molecules restricting cytotoxic t lymphocyte responses to a theileria parva vaccine candidate |
| topic | vaccines theileria parva |
| url | https://hdl.handle.net/10568/79532 |
| work_keys_str_mv | AT mwakubambanyars identificationofbolaclassimoleculesrestrictingcytotoxictlymphocyteresponsestoatheileriaparvavaccinecandidate |