Identification of microsatellite markers linked to drought tolerance in rice (Oryza sativa L.) through bulked line analysis
Identification of donor parents and markers for traits conferring drought tolerance eventually hastens the breeding for drought tolerance in rice. The extent of variation for water stress indicators, physio-morphological and plant production traits were assessed by evaluating 36 rice genotypes under...
| Main Authors: | , , , , |
|---|---|
| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Diva Enterprises Private Limited
2016
|
| Online Access: | https://hdl.handle.net/10568/79452 |
| _version_ | 1855515308283920384 |
|---|---|
| author | Rajendra Prasad, N. S. Suresh, R. Selvaraj, Michael Gomez Chandra Babu, R. Shanmugasundaram, P. |
| author_browse | Chandra Babu, R. Rajendra Prasad, N. S. Selvaraj, Michael Gomez Shanmugasundaram, P. Suresh, R. |
| author_facet | Rajendra Prasad, N. S. Suresh, R. Selvaraj, Michael Gomez Chandra Babu, R. Shanmugasundaram, P. |
| author_sort | Rajendra Prasad, N. S. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Identification of donor parents and markers for traits conferring drought tolerance eventually hastens the breeding for drought tolerance in rice. The extent of variation for water stress indicators, physio-morphological and plant production traits were assessed by evaluating 36 rice genotypes under water stress condition. Significant variation was observed for these traits and thirteen drought tolerant and susceptible genotypes were selected and grouped as tolerant and susceptible bulks based on physio-morphological and biochemical characterization. Genomic DNA was isolated from these rice accessions and pooled, respectively into drought tolerant and susceptible bulks. Bulked line analysis was carried to identify microsatellite markers linked to drought tolerance in rice. Out of 200 SSR primers screened RM 1092; RM 129 and RM157B were polymorphic between the bulks and also co-segregated among the individual genotypes constituting the respective bulks. The genomic regions flanked by these markers have been identified to be associated with various drought tolerance traits in rice and can be useful for marker assisted selection for drought resistance improvement in rice. The genotypes identified as drought tolerant can be used us donars in drought improvement breeding programmes. |
| format | Journal Article |
| id | CGSpace79452 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | Diva Enterprises Private Limited |
| publisherStr | Diva Enterprises Private Limited |
| record_format | dspace |
| spelling | CGSpace794522025-03-13T09:43:58Z Identification of microsatellite markers linked to drought tolerance in rice (Oryza sativa L.) through bulked line analysis Rajendra Prasad, N. S. Suresh, R. Selvaraj, Michael Gomez Chandra Babu, R. Shanmugasundaram, P. Identification of donor parents and markers for traits conferring drought tolerance eventually hastens the breeding for drought tolerance in rice. The extent of variation for water stress indicators, physio-morphological and plant production traits were assessed by evaluating 36 rice genotypes under water stress condition. Significant variation was observed for these traits and thirteen drought tolerant and susceptible genotypes were selected and grouped as tolerant and susceptible bulks based on physio-morphological and biochemical characterization. Genomic DNA was isolated from these rice accessions and pooled, respectively into drought tolerant and susceptible bulks. Bulked line analysis was carried to identify microsatellite markers linked to drought tolerance in rice. Out of 200 SSR primers screened RM 1092; RM 129 and RM157B were polymorphic between the bulks and also co-segregated among the individual genotypes constituting the respective bulks. The genomic regions flanked by these markers have been identified to be associated with various drought tolerance traits in rice and can be useful for marker assisted selection for drought resistance improvement in rice. The genotypes identified as drought tolerant can be used us donars in drought improvement breeding programmes. 2016-12 2017-01-30T19:06:16Z 2017-01-30T19:06:16Z Journal Article https://hdl.handle.net/10568/79452 en Open Access Diva Enterprises Private Limited Rajendra Prasad, N. S.; Suresh, R.; Gomez Selvaraj, Michael; Chandra Babu, R.; Shanmugasundaram, P.. 2016. Identification of microsatellite markers linked to drought tolerance in rice (Oryza sativa L.) through bulked line analysis. Electronic Journal of Plant Breeding. 7(4): 895-903. |
| spellingShingle | Rajendra Prasad, N. S. Suresh, R. Selvaraj, Michael Gomez Chandra Babu, R. Shanmugasundaram, P. Identification of microsatellite markers linked to drought tolerance in rice (Oryza sativa L.) through bulked line analysis |
| title | Identification of microsatellite markers linked to drought tolerance in rice (Oryza sativa L.) through bulked line analysis |
| title_full | Identification of microsatellite markers linked to drought tolerance in rice (Oryza sativa L.) through bulked line analysis |
| title_fullStr | Identification of microsatellite markers linked to drought tolerance in rice (Oryza sativa L.) through bulked line analysis |
| title_full_unstemmed | Identification of microsatellite markers linked to drought tolerance in rice (Oryza sativa L.) through bulked line analysis |
| title_short | Identification of microsatellite markers linked to drought tolerance in rice (Oryza sativa L.) through bulked line analysis |
| title_sort | identification of microsatellite markers linked to drought tolerance in rice oryza sativa l through bulked line analysis |
| url | https://hdl.handle.net/10568/79452 |
| work_keys_str_mv | AT rajendraprasadns identificationofmicrosatellitemarkerslinkedtodroughttoleranceinriceoryzasativalthroughbulkedlineanalysis AT sureshr identificationofmicrosatellitemarkerslinkedtodroughttoleranceinriceoryzasativalthroughbulkedlineanalysis AT selvarajmichaelgomez identificationofmicrosatellitemarkerslinkedtodroughttoleranceinriceoryzasativalthroughbulkedlineanalysis AT chandrababur identificationofmicrosatellitemarkerslinkedtodroughttoleranceinriceoryzasativalthroughbulkedlineanalysis AT shanmugasundaramp identificationofmicrosatellitemarkerslinkedtodroughttoleranceinriceoryzasativalthroughbulkedlineanalysis |