Development and field performance of nitrogen use efficient rice lines for Africa
Nitrogen (N) fertilizers are a major input cost in rice production, and its excess application leads to major environmental pollution. Development of rice varieties with improved nitrogen use efficiency (NUE) is essential for sustainable agriculture. Here, we report the results of field evaluations...
| Autores principales: | , , , , , , , , , , |
|---|---|
| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Wiley
2017
|
| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/79451 |
| _version_ | 1855518152671100928 |
|---|---|
| author | Selvaraj, Michael Gomez Valencia Ortiz, Milton Orlando Ogawa, Satoshi Lu, Yingzhi Wu, Liying Downs, Christopher Skinner, Wayne Lu, Zhongjin Kridl, Jean C. Ishitani, Manabu Boxtel, Jos van |
| author_browse | Boxtel, Jos van Downs, Christopher Ishitani, Manabu Kridl, Jean C. Lu, Yingzhi Lu, Zhongjin Ogawa, Satoshi Selvaraj, Michael Gomez Skinner, Wayne Valencia Ortiz, Milton Orlando Wu, Liying |
| author_facet | Selvaraj, Michael Gomez Valencia Ortiz, Milton Orlando Ogawa, Satoshi Lu, Yingzhi Wu, Liying Downs, Christopher Skinner, Wayne Lu, Zhongjin Kridl, Jean C. Ishitani, Manabu Boxtel, Jos van |
| author_sort | Selvaraj, Michael Gomez |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Nitrogen (N) fertilizers are a major input cost in rice production, and its excess application leads to major environmental pollution. Development of rice varieties with improved nitrogen use efficiency (NUE) is essential for sustainable agriculture. Here, we report the results of field evaluations of marker-free transgenic NERICA4 (New Rice for Africa 4) rice lines overexpressing barley alanine amino transferase (HvAlaAT) under the control of a rice stress-inducible promoter (pOsAnt1). Field evaluations over three growing seasons and two rice growing ecologies (lowland and upland) revealed that grain yield of pOsAnt1:HvAlaAT transgenic events was significantly higher than sibling nulls and wild-type controls under different N application rates.
Our field results clearly demonstrated that this genetic modification can significantly increase the dry biomass and grain yield compared to controls under limited N supply. Increased yield in transgenic events was correlated with increased tiller and panicle number in the field, and evidence of early establishment of a vigorous root system in hydroponic growth. Our results suggest that expression of the HvAlaAT gene can improve NUE in rice without causing undesirable growth phenotypes. The NUE technology described in this article has the potential to significantly reduce the need for N fertilizer and simultaneously improve food security, augment farm economics and mitigate greenhouse gas emissions from the rice ecosystem. |
| format | Journal Article |
| id | CGSpace79451 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace794512025-03-13T09:44:02Z Development and field performance of nitrogen use efficient rice lines for Africa Selvaraj, Michael Gomez Valencia Ortiz, Milton Orlando Ogawa, Satoshi Lu, Yingzhi Wu, Liying Downs, Christopher Skinner, Wayne Lu, Zhongjin Kridl, Jean C. Ishitani, Manabu Boxtel, Jos van oryza sativa rice alanine aminotransferase field experimentation grain yield transgenics nitrogen fertilizers transformation arroz alanina aminotransferasa experimentación en campo granos rendimiento transgénicos abonos nitrogenados biotechnology Nitrogen (N) fertilizers are a major input cost in rice production, and its excess application leads to major environmental pollution. Development of rice varieties with improved nitrogen use efficiency (NUE) is essential for sustainable agriculture. Here, we report the results of field evaluations of marker-free transgenic NERICA4 (New Rice for Africa 4) rice lines overexpressing barley alanine amino transferase (HvAlaAT) under the control of a rice stress-inducible promoter (pOsAnt1). Field evaluations over three growing seasons and two rice growing ecologies (lowland and upland) revealed that grain yield of pOsAnt1:HvAlaAT transgenic events was significantly higher than sibling nulls and wild-type controls under different N application rates. Our field results clearly demonstrated that this genetic modification can significantly increase the dry biomass and grain yield compared to controls under limited N supply. Increased yield in transgenic events was correlated with increased tiller and panicle number in the field, and evidence of early establishment of a vigorous root system in hydroponic growth. Our results suggest that expression of the HvAlaAT gene can improve NUE in rice without causing undesirable growth phenotypes. The NUE technology described in this article has the potential to significantly reduce the need for N fertilizer and simultaneously improve food security, augment farm economics and mitigate greenhouse gas emissions from the rice ecosystem. 2017-06 2017-01-30T19:05:54Z 2017-01-30T19:05:54Z Journal Article https://hdl.handle.net/10568/79451 en Open Access Wiley Gomez Selvaraj, Michael; Valencia, Milton Orlando; Ogawa, Satoshi; Lu, Yingzhi; Wu, Liying; Downs, Christopher; Skinner, Wayne; Lu, Zhongjin; Kridl, Jean C.; Ishitani, Manabu; Boxtel, Jos Van. 2017. Development and field performance of nitrogen use efficient rice lines for Africa . Plant Biotechnology Journal . Wiley-Blackwell Publishing Ltd., 15(6): 775-787 |
| spellingShingle | oryza sativa rice alanine aminotransferase field experimentation grain yield transgenics nitrogen fertilizers transformation arroz alanina aminotransferasa experimentación en campo granos rendimiento transgénicos abonos nitrogenados biotechnology Selvaraj, Michael Gomez Valencia Ortiz, Milton Orlando Ogawa, Satoshi Lu, Yingzhi Wu, Liying Downs, Christopher Skinner, Wayne Lu, Zhongjin Kridl, Jean C. Ishitani, Manabu Boxtel, Jos van Development and field performance of nitrogen use efficient rice lines for Africa |
| title | Development and field performance of nitrogen use efficient rice lines for Africa |
| title_full | Development and field performance of nitrogen use efficient rice lines for Africa |
| title_fullStr | Development and field performance of nitrogen use efficient rice lines for Africa |
| title_full_unstemmed | Development and field performance of nitrogen use efficient rice lines for Africa |
| title_short | Development and field performance of nitrogen use efficient rice lines for Africa |
| title_sort | development and field performance of nitrogen use efficient rice lines for africa |
| topic | oryza sativa rice alanine aminotransferase field experimentation grain yield transgenics nitrogen fertilizers transformation arroz alanina aminotransferasa experimentación en campo granos rendimiento transgénicos abonos nitrogenados biotechnology |
| url | https://hdl.handle.net/10568/79451 |
| work_keys_str_mv | AT selvarajmichaelgomez developmentandfieldperformanceofnitrogenuseefficientricelinesforafrica AT valenciaortizmiltonorlando developmentandfieldperformanceofnitrogenuseefficientricelinesforafrica AT ogawasatoshi developmentandfieldperformanceofnitrogenuseefficientricelinesforafrica AT luyingzhi developmentandfieldperformanceofnitrogenuseefficientricelinesforafrica AT wuliying developmentandfieldperformanceofnitrogenuseefficientricelinesforafrica AT downschristopher developmentandfieldperformanceofnitrogenuseefficientricelinesforafrica AT skinnerwayne developmentandfieldperformanceofnitrogenuseefficientricelinesforafrica AT luzhongjin developmentandfieldperformanceofnitrogenuseefficientricelinesforafrica AT kridljeanc developmentandfieldperformanceofnitrogenuseefficientricelinesforafrica AT ishitanimanabu developmentandfieldperformanceofnitrogenuseefficientricelinesforafrica AT boxteljosvan developmentandfieldperformanceofnitrogenuseefficientricelinesforafrica |