Viral class 1 RNase III involved in suppression of RNA silencing
Double-stranded RNA (dsRNA)-specific endonucleases belonging to RNase III classes 3 and 2 process dsRNA precursors to small interfering RNA (siRNA) or microRNA, respectively, thereby initiating and amplifying RNA silencing-based antiviral defense and gene regulation in eukaryotic cells. However, we...
| Main Authors: | , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
American Society for Microbiology
2005
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/79401 |
| _version_ | 1855514749795565568 |
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| author | Kreuze, Jan F. Savenkov, E.I. Cuéllar, Wilmer Jose Xiangdong Li Valkonen, J.P.T. |
| author_browse | Cuéllar, Wilmer Jose Kreuze, Jan F. Savenkov, E.I. Valkonen, J.P.T. Xiangdong Li |
| author_facet | Kreuze, Jan F. Savenkov, E.I. Cuéllar, Wilmer Jose Xiangdong Li Valkonen, J.P.T. |
| author_sort | Kreuze, Jan F. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Double-stranded RNA (dsRNA)-specific endonucleases belonging to RNase III classes 3 and 2 process dsRNA precursors to small interfering RNA (siRNA) or microRNA, respectively, thereby initiating and amplifying RNA silencing-based antiviral defense and gene regulation in eukaryotic cells. However, we now provide evidence that a class 1 RNase III is involved in suppression of RNA silencing. The single-stranded RNA genome of sweet potato chlorotic stunt virus (SPCSV) encodes an RNase III (RNase3) homologous to putative class 1 RNase IIIs of unknown function in rice and Arabidopsis. We show that RNase3 has dsRNA-specific endonuclease activity that enhances the RNA-silencing suppression activity of another protein (p22) encoded by SPCSV. RNase3 and p22 coexpression reduced siRNA accumulation more efficiently than p22 alone in Nicotiana benthamiana leaves expressing a strong silencing inducer (i.e., dsRNA). RNase3 did not cause intracellular silencing suppression or reduce accumulation of siRNA in the absence of p22 or enhance silencing suppression activity of a protein encoded by a heterologous virus. No other known RNA virus encodes an RNase III or uses two independent proteins cooperatively for RNA silencing suppression. |
| format | Journal Article |
| id | CGSpace79401 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2005 |
| publishDateRange | 2005 |
| publishDateSort | 2005 |
| publisher | American Society for Microbiology |
| publisherStr | American Society for Microbiology |
| record_format | dspace |
| spelling | CGSpace794012024-04-25T06:00:18Z Viral class 1 RNase III involved in suppression of RNA silencing Kreuze, Jan F. Savenkov, E.I. Cuéllar, Wilmer Jose Xiangdong Li Valkonen, J.P.T. viruses research virology immunology microbiology Double-stranded RNA (dsRNA)-specific endonucleases belonging to RNase III classes 3 and 2 process dsRNA precursors to small interfering RNA (siRNA) or microRNA, respectively, thereby initiating and amplifying RNA silencing-based antiviral defense and gene regulation in eukaryotic cells. However, we now provide evidence that a class 1 RNase III is involved in suppression of RNA silencing. The single-stranded RNA genome of sweet potato chlorotic stunt virus (SPCSV) encodes an RNase III (RNase3) homologous to putative class 1 RNase IIIs of unknown function in rice and Arabidopsis. We show that RNase3 has dsRNA-specific endonuclease activity that enhances the RNA-silencing suppression activity of another protein (p22) encoded by SPCSV. RNase3 and p22 coexpression reduced siRNA accumulation more efficiently than p22 alone in Nicotiana benthamiana leaves expressing a strong silencing inducer (i.e., dsRNA). RNase3 did not cause intracellular silencing suppression or reduce accumulation of siRNA in the absence of p22 or enhance silencing suppression activity of a protein encoded by a heterologous virus. No other known RNA virus encodes an RNase III or uses two independent proteins cooperatively for RNA silencing suppression. 2005-06 2017-01-26T14:21:09Z 2017-01-26T14:21:09Z Journal Article https://hdl.handle.net/10568/79401 en Limited Access American Society for Microbiology Kreuze, J.F.; Savenkov, E.I.; Cuellar, W.; Li, X.; Valkonen, J.P.T. 2005. Viral class 1 RNase III involved in suppression of RNA silencing. Journal of Virology. (USA). ISSN 0022-538X. 79(11):7227-7238. |
| spellingShingle | viruses research virology immunology microbiology Kreuze, Jan F. Savenkov, E.I. Cuéllar, Wilmer Jose Xiangdong Li Valkonen, J.P.T. Viral class 1 RNase III involved in suppression of RNA silencing |
| title | Viral class 1 RNase III involved in suppression of RNA silencing |
| title_full | Viral class 1 RNase III involved in suppression of RNA silencing |
| title_fullStr | Viral class 1 RNase III involved in suppression of RNA silencing |
| title_full_unstemmed | Viral class 1 RNase III involved in suppression of RNA silencing |
| title_short | Viral class 1 RNase III involved in suppression of RNA silencing |
| title_sort | viral class 1 rnase iii involved in suppression of rna silencing |
| topic | viruses research virology immunology microbiology |
| url | https://hdl.handle.net/10568/79401 |
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