Elimination of antiviral defense by viral RNase III
Sweet potato (Ipomoea batatas) is an important subsistence and famine reserve crop grown in developing countries where Sweet potato chlorotic stunt virus (SPCSV; Closteroviridae), a single-stranded RNA (ssRNA) crinivirus, synergizes unrelated viruses in co-infected sweet potato plants. The most seve...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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National Academy of Sciences
2009
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/79398 |
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| author | Cuéllar, Wilmer Jose Kreuze, Jan F. Rajamaki, M.L. Cruzado, K.R. Untiveros, M. Valkonen, J.P.T. |
| author_browse | Cruzado, K.R. Cuéllar, Wilmer Jose Kreuze, Jan F. Rajamaki, M.L. Untiveros, M. Valkonen, J.P.T. |
| author_facet | Cuéllar, Wilmer Jose Kreuze, Jan F. Rajamaki, M.L. Cruzado, K.R. Untiveros, M. Valkonen, J.P.T. |
| author_sort | Cuéllar, Wilmer Jose |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Sweet potato (Ipomoea batatas) is an important subsistence and famine reserve crop grown in developing countries where Sweet potato chlorotic stunt virus (SPCSV; Closteroviridae), a single-stranded RNA (ssRNA) crinivirus, synergizes unrelated viruses in co-infected sweet potato plants. The most severe disease and yield losses are caused by co-infection with SPCSV and a potyvirus, Sweet potato feathery mottle virus (SPFMV; Potyviridae). Potyviruses synergize unrelated viruses by suppression of RNA silencing with the P1/HC-Pro polyprotein; however, the SPCSV-SPFMV synergism is unusual in that the potyvirus is the beneficiary. Our data show that transformation of an SPFMV-resistant sweet potato variety with the double-stranded RNA (dsRNA)-specific class 1 RNA endoribonuclease III (RNase3) of SPCSV broke down resistance to SPFMV, leading to high accumulation of SPFMV antigen and severe disease symptoms similar to the synergism in plants co-infected with SPCSV and SPFMV. RNase3-transgenic sweet potatoes also accumulated higher concentrations of 2 other unrelated viruses and developed more severe symptoms than non-transgenic plants. In leaves, RNase3 suppressed ssRNA-induced gene silencing (RNAi) in an endonuclease activity-dependent manner. It cleaved synthetic double-stranded small interfering RNAs (siRNAs) of 21, 22, and 24 bp in vitro to products of approximately 14 bp that are inactive in RNAi. It also affected total siRNA isolated from SPFMV-infected sweet potato plants, suggesting a viral mechanism for suppression of RNAi by cleavage of siRNA. Results implicate RNase3 in suppression of antiviral defense in sweet potato plants and reveal RNase3 as a protein that mediates viral synergism with several unrelated viruses, a function previously described only for P1/HC-Pro. |
| format | Journal Article |
| id | CGSpace79398 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2009 |
| publishDateRange | 2009 |
| publishDateSort | 2009 |
| publisher | National Academy of Sciences |
| publisherStr | National Academy of Sciences |
| record_format | dspace |
| spelling | CGSpace793982024-10-17T09:47:53Z Elimination of antiviral defense by viral RNase III Cuéllar, Wilmer Jose Kreuze, Jan F. Rajamaki, M.L. Cruzado, K.R. Untiveros, M. Valkonen, J.P.T. animal diseases viruses Sweet potato (Ipomoea batatas) is an important subsistence and famine reserve crop grown in developing countries where Sweet potato chlorotic stunt virus (SPCSV; Closteroviridae), a single-stranded RNA (ssRNA) crinivirus, synergizes unrelated viruses in co-infected sweet potato plants. The most severe disease and yield losses are caused by co-infection with SPCSV and a potyvirus, Sweet potato feathery mottle virus (SPFMV; Potyviridae). Potyviruses synergize unrelated viruses by suppression of RNA silencing with the P1/HC-Pro polyprotein; however, the SPCSV-SPFMV synergism is unusual in that the potyvirus is the beneficiary. Our data show that transformation of an SPFMV-resistant sweet potato variety with the double-stranded RNA (dsRNA)-specific class 1 RNA endoribonuclease III (RNase3) of SPCSV broke down resistance to SPFMV, leading to high accumulation of SPFMV antigen and severe disease symptoms similar to the synergism in plants co-infected with SPCSV and SPFMV. RNase3-transgenic sweet potatoes also accumulated higher concentrations of 2 other unrelated viruses and developed more severe symptoms than non-transgenic plants. In leaves, RNase3 suppressed ssRNA-induced gene silencing (RNAi) in an endonuclease activity-dependent manner. It cleaved synthetic double-stranded small interfering RNAs (siRNAs) of 21, 22, and 24 bp in vitro to products of approximately 14 bp that are inactive in RNAi. It also affected total siRNA isolated from SPFMV-infected sweet potato plants, suggesting a viral mechanism for suppression of RNAi by cleavage of siRNA. Results implicate RNase3 in suppression of antiviral defense in sweet potato plants and reveal RNase3 as a protein that mediates viral synergism with several unrelated viruses, a function previously described only for P1/HC-Pro. 2009-06-23 2017-01-26T13:33:09Z 2017-01-26T13:33:09Z Journal Article https://hdl.handle.net/10568/79398 en Open Access National Academy of Sciences Cuellar, W.J.; Kreuze, J.F.; Rajamaki, M.L.; Cruzado, K.R.; Untiveros, M.; Valkonen, J.P.T. 2009. Elimination of antiviral defense by viral RNase III. Proceedings National Academy of Sciences (PNAS). (USA). ISSN 0027-8424. 106(25):10354-10358. |
| spellingShingle | animal diseases viruses Cuéllar, Wilmer Jose Kreuze, Jan F. Rajamaki, M.L. Cruzado, K.R. Untiveros, M. Valkonen, J.P.T. Elimination of antiviral defense by viral RNase III |
| title | Elimination of antiviral defense by viral RNase III |
| title_full | Elimination of antiviral defense by viral RNase III |
| title_fullStr | Elimination of antiviral defense by viral RNase III |
| title_full_unstemmed | Elimination of antiviral defense by viral RNase III |
| title_short | Elimination of antiviral defense by viral RNase III |
| title_sort | elimination of antiviral defense by viral rnase iii |
| topic | animal diseases viruses |
| url | https://hdl.handle.net/10568/79398 |
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