Genomic analysis identified a potential novel molecular mechanism for high-altitude adaptation in sheep at the Himalayas
Sheep has successfully adapted to the extreme high-altitude Himalayan region. To identify genes underlying such adaptation, we genotyped genome-wide single nucleotide polymorphisms (SNPs) of four major sheep breeds living at different altitudes in Nepal and downloaded SNP array data from additional...
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Springer
2016
|
| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/78442 |
| _version_ | 1855522036810514432 |
|---|---|
| author | Gorkhali, N.A. Kunzhe Dong Min Yang Shen Song Kader, A. Shrestha, B.S. Xiaohong He Qian-Jun Zhao Yabin Pu Xiangchen Li Kijas, J. Weijun, Guan Han Jianlin Lin Jiang Yuehui Ma |
| author_browse | Gorkhali, N.A. Han Jianlin Kader, A. Kijas, J. Kunzhe Dong Lin Jiang Min Yang Qian-Jun Zhao Shen Song Shrestha, B.S. Weijun, Guan Xiangchen Li Xiaohong He Yabin Pu Yuehui Ma |
| author_facet | Gorkhali, N.A. Kunzhe Dong Min Yang Shen Song Kader, A. Shrestha, B.S. Xiaohong He Qian-Jun Zhao Yabin Pu Xiangchen Li Kijas, J. Weijun, Guan Han Jianlin Lin Jiang Yuehui Ma |
| author_sort | Gorkhali, N.A. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Sheep has successfully adapted to the extreme high-altitude Himalayan region. To identify genes underlying such adaptation, we genotyped genome-wide single nucleotide polymorphisms (SNPs) of four major sheep breeds living at different altitudes in Nepal and downloaded SNP array data from additional Asian and Middle East breeds. Using a di value-based genomic comparison between four high-altitude and eight lowland Asian breeds, we discovered the most differentiated variants at the locus of FGF-7 (Keratinocyte growth factor-7), which was previously reported as a good protective candidate for pulmonary injuries. We further found a SNP upstream of FGF-7 that appears to contribute to the divergence signature. First, the SNP occurred at an extremely conserved site. Second, the SNP showed an increasing allele frequency with the elevated altitude in Nepalese sheep. Third, the electrophoretic mobility shift assays (EMSA) analysis using human lung cancer cells revealed the allele-specific DNA-protein interactions. We thus hypothesized that FGF-7 gene potentially enhances lung function by regulating its expression level in high-altitude sheep through altering its binding of specific transcription factors. Especially, FGF-7 gene was not implicated in previous studies of other high-altitude species, suggesting a potential novel adaptive mechanism to high altitude in sheep at the Himalayas. |
| format | Journal Article |
| id | CGSpace78442 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace784422024-05-01T08:16:37Z Genomic analysis identified a potential novel molecular mechanism for high-altitude adaptation in sheep at the Himalayas Gorkhali, N.A. Kunzhe Dong Min Yang Shen Song Kader, A. Shrestha, B.S. Xiaohong He Qian-Jun Zhao Yabin Pu Xiangchen Li Kijas, J. Weijun, Guan Han Jianlin Lin Jiang Yuehui Ma breeds livestock sheep Sheep has successfully adapted to the extreme high-altitude Himalayan region. To identify genes underlying such adaptation, we genotyped genome-wide single nucleotide polymorphisms (SNPs) of four major sheep breeds living at different altitudes in Nepal and downloaded SNP array data from additional Asian and Middle East breeds. Using a di value-based genomic comparison between four high-altitude and eight lowland Asian breeds, we discovered the most differentiated variants at the locus of FGF-7 (Keratinocyte growth factor-7), which was previously reported as a good protective candidate for pulmonary injuries. We further found a SNP upstream of FGF-7 that appears to contribute to the divergence signature. First, the SNP occurred at an extremely conserved site. Second, the SNP showed an increasing allele frequency with the elevated altitude in Nepalese sheep. Third, the electrophoretic mobility shift assays (EMSA) analysis using human lung cancer cells revealed the allele-specific DNA-protein interactions. We thus hypothesized that FGF-7 gene potentially enhances lung function by regulating its expression level in high-altitude sheep through altering its binding of specific transcription factors. Especially, FGF-7 gene was not implicated in previous studies of other high-altitude species, suggesting a potential novel adaptive mechanism to high altitude in sheep at the Himalayas. 2016-07-22 2016-12-21T13:08:05Z 2016-12-21T13:08:05Z Journal Article https://hdl.handle.net/10568/78442 en Open Access Springer Gorkhali, N.A., Kunzhe Dong, Min Yang, Shen Song, Kader, A., Shrestha, B.S., Xiaohong He, Qianjun Zhao, Yabin Pu, Xiangchen Li, Kijas, J., Weijun Guan, Jianlin Han, Lin Jiang and Yuehui Ma. 2016. Genomic analysis identified a potential novel molecular mechanism for high-altitude adaptation in sheep at the Himalayas. Scientific Reports 6: 29963. |
| spellingShingle | breeds livestock sheep Gorkhali, N.A. Kunzhe Dong Min Yang Shen Song Kader, A. Shrestha, B.S. Xiaohong He Qian-Jun Zhao Yabin Pu Xiangchen Li Kijas, J. Weijun, Guan Han Jianlin Lin Jiang Yuehui Ma Genomic analysis identified a potential novel molecular mechanism for high-altitude adaptation in sheep at the Himalayas |
| title | Genomic analysis identified a potential novel molecular mechanism for high-altitude adaptation in sheep at the Himalayas |
| title_full | Genomic analysis identified a potential novel molecular mechanism for high-altitude adaptation in sheep at the Himalayas |
| title_fullStr | Genomic analysis identified a potential novel molecular mechanism for high-altitude adaptation in sheep at the Himalayas |
| title_full_unstemmed | Genomic analysis identified a potential novel molecular mechanism for high-altitude adaptation in sheep at the Himalayas |
| title_short | Genomic analysis identified a potential novel molecular mechanism for high-altitude adaptation in sheep at the Himalayas |
| title_sort | genomic analysis identified a potential novel molecular mechanism for high altitude adaptation in sheep at the himalayas |
| topic | breeds livestock sheep |
| url | https://hdl.handle.net/10568/78442 |
| work_keys_str_mv | AT gorkhalina genomicanalysisidentifiedapotentialnovelmolecularmechanismforhighaltitudeadaptationinsheepatthehimalayas AT kunzhedong genomicanalysisidentifiedapotentialnovelmolecularmechanismforhighaltitudeadaptationinsheepatthehimalayas AT minyang genomicanalysisidentifiedapotentialnovelmolecularmechanismforhighaltitudeadaptationinsheepatthehimalayas AT shensong genomicanalysisidentifiedapotentialnovelmolecularmechanismforhighaltitudeadaptationinsheepatthehimalayas AT kadera genomicanalysisidentifiedapotentialnovelmolecularmechanismforhighaltitudeadaptationinsheepatthehimalayas AT shresthabs genomicanalysisidentifiedapotentialnovelmolecularmechanismforhighaltitudeadaptationinsheepatthehimalayas AT xiaohonghe genomicanalysisidentifiedapotentialnovelmolecularmechanismforhighaltitudeadaptationinsheepatthehimalayas AT qianjunzhao genomicanalysisidentifiedapotentialnovelmolecularmechanismforhighaltitudeadaptationinsheepatthehimalayas AT yabinpu genomicanalysisidentifiedapotentialnovelmolecularmechanismforhighaltitudeadaptationinsheepatthehimalayas AT xiangchenli genomicanalysisidentifiedapotentialnovelmolecularmechanismforhighaltitudeadaptationinsheepatthehimalayas AT kijasj genomicanalysisidentifiedapotentialnovelmolecularmechanismforhighaltitudeadaptationinsheepatthehimalayas AT weijunguan genomicanalysisidentifiedapotentialnovelmolecularmechanismforhighaltitudeadaptationinsheepatthehimalayas AT hanjianlin genomicanalysisidentifiedapotentialnovelmolecularmechanismforhighaltitudeadaptationinsheepatthehimalayas AT linjiang genomicanalysisidentifiedapotentialnovelmolecularmechanismforhighaltitudeadaptationinsheepatthehimalayas AT yuehuima genomicanalysisidentifiedapotentialnovelmolecularmechanismforhighaltitudeadaptationinsheepatthehimalayas |