Rapid identification of bovine MHCI haplotypes in genetically divergent cattle populations using next-generation sequencing
The major histocompatibility complex (MHC) region contains many genes that are key regulators of both innate and adaptive immunity including the polymorphic MHCI and MHCII genes. Consequently, the characterisation of the repertoire of MHC genes is critical to understanding the variation that determi...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Springer
2016
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/78440 |
| _version_ | 1855541253604638720 |
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| author | Vasoya, D. Law, A. Motta, P. Mingyan Yu Muwonge, A. Cook, Elizabeth A.J. Xiaoying Li Bryson, K. MacCallam, A. Sitt, Tatjana Toye, Philip G. Bronsvoort, B.M. de C. Watson, Mick Morrison, W. Ivan Connelley, T. |
| author_browse | Bronsvoort, B.M. de C. Bryson, K. Connelley, T. Cook, Elizabeth A.J. Law, A. MacCallam, A. Mingyan Yu Morrison, W. Ivan Motta, P. Muwonge, A. Sitt, Tatjana Toye, Philip G. Vasoya, D. Watson, Mick Xiaoying Li |
| author_facet | Vasoya, D. Law, A. Motta, P. Mingyan Yu Muwonge, A. Cook, Elizabeth A.J. Xiaoying Li Bryson, K. MacCallam, A. Sitt, Tatjana Toye, Philip G. Bronsvoort, B.M. de C. Watson, Mick Morrison, W. Ivan Connelley, T. |
| author_sort | Vasoya, D. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The major histocompatibility complex (MHC) region contains many genes that are key regulators of both innate and adaptive immunity including the polymorphic MHCI and MHCII genes. Consequently, the characterisation of the repertoire of MHC genes is critical to understanding the variation that determines the nature of immune responses. Our current knowledge of the bovine MHCI repertoire is limited with only the Holstein-Friesian breed having been studied in any depth. Traditional methods of MHCI genotyping are of low resolution and laborious and this has been a major impediment to a more comprehensive analysis of the MHCI repertoire of other cattle breeds. Next-generation sequencing (NGS) technologies have been used to enable high throughput and much higher resolution MHCI typing in a number of species. In this study we have developed a MiSeq platform approach and requisite bioinformatics pipeline to facilitate typing of bovine MHCI repertoires. The method was validated initially on a cohort of Holstein-Friesian animals and then demonstrated to enable characterisation of MHCI repertoires in African cattle breeds, for which there was limited or no available data. During the course of these studies we identified >140 novel classical MHCI genes and defined 62 novel MHCI haplotypes, dramatically expanding the known bovine MHCI repertoire. |
| format | Journal Article |
| id | CGSpace78440 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace784402024-05-01T08:19:51Z Rapid identification of bovine MHCI haplotypes in genetically divergent cattle populations using next-generation sequencing Vasoya, D. Law, A. Motta, P. Mingyan Yu Muwonge, A. Cook, Elizabeth A.J. Xiaoying Li Bryson, K. MacCallam, A. Sitt, Tatjana Toye, Philip G. Bronsvoort, B.M. de C. Watson, Mick Morrison, W. Ivan Connelley, T. cattle identification major histocompatibility complex animal diseases The major histocompatibility complex (MHC) region contains many genes that are key regulators of both innate and adaptive immunity including the polymorphic MHCI and MHCII genes. Consequently, the characterisation of the repertoire of MHC genes is critical to understanding the variation that determines the nature of immune responses. Our current knowledge of the bovine MHCI repertoire is limited with only the Holstein-Friesian breed having been studied in any depth. Traditional methods of MHCI genotyping are of low resolution and laborious and this has been a major impediment to a more comprehensive analysis of the MHCI repertoire of other cattle breeds. Next-generation sequencing (NGS) technologies have been used to enable high throughput and much higher resolution MHCI typing in a number of species. In this study we have developed a MiSeq platform approach and requisite bioinformatics pipeline to facilitate typing of bovine MHCI repertoires. The method was validated initially on a cohort of Holstein-Friesian animals and then demonstrated to enable characterisation of MHCI repertoires in African cattle breeds, for which there was limited or no available data. During the course of these studies we identified >140 novel classical MHCI genes and defined 62 novel MHCI haplotypes, dramatically expanding the known bovine MHCI repertoire. 2016-11 2016-12-21T13:06:42Z 2016-12-21T13:06:42Z Journal Article https://hdl.handle.net/10568/78440 en Open Access Springer Vasoya, D., Law, A., Motta, P., Mingyan Yu, Muwonge, A., Cook, E., Xiaoying Li, Bryson, K., MacCallam, A., Sitt, T., Toye, P., Bronsvoort, B., Watson, M., Morrison, W.I. and Connelley, T. 2016. Rapid identification of bovine MHCI haplotypes in genetically divergent cattle populations using next-generation sequencing. Immunogenetics 68 (10):765–781. |
| spellingShingle | cattle identification major histocompatibility complex animal diseases Vasoya, D. Law, A. Motta, P. Mingyan Yu Muwonge, A. Cook, Elizabeth A.J. Xiaoying Li Bryson, K. MacCallam, A. Sitt, Tatjana Toye, Philip G. Bronsvoort, B.M. de C. Watson, Mick Morrison, W. Ivan Connelley, T. Rapid identification of bovine MHCI haplotypes in genetically divergent cattle populations using next-generation sequencing |
| title | Rapid identification of bovine MHCI haplotypes in genetically divergent cattle populations using next-generation sequencing |
| title_full | Rapid identification of bovine MHCI haplotypes in genetically divergent cattle populations using next-generation sequencing |
| title_fullStr | Rapid identification of bovine MHCI haplotypes in genetically divergent cattle populations using next-generation sequencing |
| title_full_unstemmed | Rapid identification of bovine MHCI haplotypes in genetically divergent cattle populations using next-generation sequencing |
| title_short | Rapid identification of bovine MHCI haplotypes in genetically divergent cattle populations using next-generation sequencing |
| title_sort | rapid identification of bovine mhci haplotypes in genetically divergent cattle populations using next generation sequencing |
| topic | cattle identification major histocompatibility complex animal diseases |
| url | https://hdl.handle.net/10568/78440 |
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