Expression profiling and functional characterization of miR-192 throughout sheep skeletal muscle development
MicroRNAs (miRNAs) are evolutionarily conserved, small, non-coding RNAs that have emerged as key regulators of myogenesis. Here, we examined the miRNA expression profiles of developing sheep skeletal muscle using a deep sequencing approach. We detected 2,396 miRNAs in the sheep skeletal muscle tissu...
| Autores principales: | , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2016
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/78439 |
| _version_ | 1855538216867725312 |
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| author | Qian-Jun Zhao Ye Kang Hong-Yang Wang Weijun, Guan Xiang-Chen Li Lin Jiang Xiao-Hong He Yabin Pu Han Jianlin Yue-Hui Ma |
| author_browse | Han Jianlin Hong-Yang Wang Lin Jiang Qian-Jun Zhao Weijun, Guan Xiang-Chen Li Xiao-Hong He Yabin Pu Ye Kang Yue-Hui Ma |
| author_facet | Qian-Jun Zhao Ye Kang Hong-Yang Wang Weijun, Guan Xiang-Chen Li Lin Jiang Xiao-Hong He Yabin Pu Han Jianlin Yue-Hui Ma |
| author_sort | Qian-Jun Zhao |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | MicroRNAs (miRNAs) are evolutionarily conserved, small, non-coding RNAs that have emerged as key regulators of myogenesis. Here, we examined the miRNA expression profiles of developing sheep skeletal muscle using a deep sequencing approach. We detected 2,396 miRNAs in the sheep skeletal muscle tissues. Of these, miR-192 was found to be up-regulated in prenatal skeletal muscle, but was down-regulated postnatally. MiR-192 expression also decreased during the myogenic differentiation of sheep satellite cells (SCs). MiR-192 overexpression significantly attenuated SCs myogenic differentiation but promoted SCs proliferation, whereas miR-192 inhibition enhanced SCs differentiation but suppressed SCs proliferation. We found that miR-192 targeted retinoblastoma 1 (RB1), a known regulator of myogenesis. Furthermore, knockdown of RB1 in cultured cells significantly inhibited SCs myogenic differentiation but accelerated SCs proliferation, confirming the role of RB1 in myogenesis. Taken together, our findings enrich the ovine miRNA database, and outline the miRNA transcriptome of sheep during skeletal muscle development. Moreover, we show that miR-192 affects SCs proliferation and myogenic differentiation via down-regulation of RB1. |
| format | Journal Article |
| id | CGSpace78439 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace784392024-05-01T08:19:24Z Expression profiling and functional characterization of miR-192 throughout sheep skeletal muscle development Qian-Jun Zhao Ye Kang Hong-Yang Wang Weijun, Guan Xiang-Chen Li Lin Jiang Xiao-Hong He Yabin Pu Han Jianlin Yue-Hui Ma muscles sheep skeletal development MicroRNAs (miRNAs) are evolutionarily conserved, small, non-coding RNAs that have emerged as key regulators of myogenesis. Here, we examined the miRNA expression profiles of developing sheep skeletal muscle using a deep sequencing approach. We detected 2,396 miRNAs in the sheep skeletal muscle tissues. Of these, miR-192 was found to be up-regulated in prenatal skeletal muscle, but was down-regulated postnatally. MiR-192 expression also decreased during the myogenic differentiation of sheep satellite cells (SCs). MiR-192 overexpression significantly attenuated SCs myogenic differentiation but promoted SCs proliferation, whereas miR-192 inhibition enhanced SCs differentiation but suppressed SCs proliferation. We found that miR-192 targeted retinoblastoma 1 (RB1), a known regulator of myogenesis. Furthermore, knockdown of RB1 in cultured cells significantly inhibited SCs myogenic differentiation but accelerated SCs proliferation, confirming the role of RB1 in myogenesis. Taken together, our findings enrich the ovine miRNA database, and outline the miRNA transcriptome of sheep during skeletal muscle development. Moreover, we show that miR-192 affects SCs proliferation and myogenic differentiation via down-regulation of RB1. 2016-07-25 2016-12-21T13:06:38Z 2016-12-21T13:06:38Z Journal Article https://hdl.handle.net/10568/78439 en Open Access Springer Qian Zhao, Ye Kang, Hong-Yang Wang, Wei-Jun Guan, Xiang-Chen Li, Lin Jiang, Xiao-Hong He, Ya-Bin Pu, Jian-Lin Han, Yue-Hui Ma and Qian-Jun Zhao. 2016. Expression profiling and functional characterization of miR-192 throughout sheep skeletal muscle development. Scientific Reports 6: 30281. |
| spellingShingle | muscles sheep skeletal development Qian-Jun Zhao Ye Kang Hong-Yang Wang Weijun, Guan Xiang-Chen Li Lin Jiang Xiao-Hong He Yabin Pu Han Jianlin Yue-Hui Ma Expression profiling and functional characterization of miR-192 throughout sheep skeletal muscle development |
| title | Expression profiling and functional characterization of miR-192 throughout sheep skeletal muscle development |
| title_full | Expression profiling and functional characterization of miR-192 throughout sheep skeletal muscle development |
| title_fullStr | Expression profiling and functional characterization of miR-192 throughout sheep skeletal muscle development |
| title_full_unstemmed | Expression profiling and functional characterization of miR-192 throughout sheep skeletal muscle development |
| title_short | Expression profiling and functional characterization of miR-192 throughout sheep skeletal muscle development |
| title_sort | expression profiling and functional characterization of mir 192 throughout sheep skeletal muscle development |
| topic | muscles sheep skeletal development |
| url | https://hdl.handle.net/10568/78439 |
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