Faecal matter as inoculum for in vitro gas production

Rumen inoculum (RI) was replaced by fresh faecal inoculum (FI) on microbial nitrogen equivalent. Microbial nitrogen in FI was estimated as total N minus N bound to neutral detergent in stover-fed bullocks and 2.8 gram of FI provided similar (6.4 mg) microbial N to that of 10 ml of RI. Extent and rat...

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Autores principales: Pandian, C.S., Reddy, T.K., Sivaiah, Blümmel, Michael, Ramana Reddy, Y.
Formato: Journal Article
Lenguaje:Inglés
Publicado: Diva Enterprises Private Limited 2016
Materias:
Acceso en línea:https://hdl.handle.net/10568/78422
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author Pandian, C.S.
Reddy, T.K.
Sivaiah
Blümmel, Michael
Ramana Reddy, Y.
author_browse Blümmel, Michael
Pandian, C.S.
Ramana Reddy, Y.
Reddy, T.K.
Sivaiah
author_facet Pandian, C.S.
Reddy, T.K.
Sivaiah
Blümmel, Michael
Ramana Reddy, Y.
author_sort Pandian, C.S.
collection Repository of Agricultural Research Outputs (CGSpace)
description Rumen inoculum (RI) was replaced by fresh faecal inoculum (FI) on microbial nitrogen equivalent. Microbial nitrogen in FI was estimated as total N minus N bound to neutral detergent in stover-fed bullocks and 2.8 gram of FI provided similar (6.4 mg) microbial N to that of 10 ml of RI. Extent and rate of in vitro gas production (IVGP) of 200 mg of standards of sorghum stover and hay was measured in syringes containing a total of 30 ml of medium with 2.5, 3.0, 3.5 or 4.0 g FI and compared with incubations containing 10 ml of RI in 30 ml of medium. Faecal concentration had little effect on extent of gas production but rate of gas production increased from 2.5 to 3.5 g but decreased from 3.5 to 4.0 g. However, rates of gas production in 3.5 g of FI were only about 60–65% of the rates observed with RI. Ranking between FI and RI agreed for rates but not always for extents of gas production. To conclude, similar amounts of microbial N provided by either rumen or faecal inoculum do not result in similar degradative potential in vitro. However, in the present scenario, FI can be used as an alternative inoculum to RI for IVGP technique.
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spelling CGSpace784222024-04-25T06:00:48Z Faecal matter as inoculum for in vitro gas production Pandian, C.S. Reddy, T.K. Sivaiah Blümmel, Michael Ramana Reddy, Y. animal feeding livestock Rumen inoculum (RI) was replaced by fresh faecal inoculum (FI) on microbial nitrogen equivalent. Microbial nitrogen in FI was estimated as total N minus N bound to neutral detergent in stover-fed bullocks and 2.8 gram of FI provided similar (6.4 mg) microbial N to that of 10 ml of RI. Extent and rate of in vitro gas production (IVGP) of 200 mg of standards of sorghum stover and hay was measured in syringes containing a total of 30 ml of medium with 2.5, 3.0, 3.5 or 4.0 g FI and compared with incubations containing 10 ml of RI in 30 ml of medium. Faecal concentration had little effect on extent of gas production but rate of gas production increased from 2.5 to 3.5 g but decreased from 3.5 to 4.0 g. However, rates of gas production in 3.5 g of FI were only about 60–65% of the rates observed with RI. Ranking between FI and RI agreed for rates but not always for extents of gas production. To conclude, similar amounts of microbial N provided by either rumen or faecal inoculum do not result in similar degradative potential in vitro. However, in the present scenario, FI can be used as an alternative inoculum to RI for IVGP technique. 2016-05-15 2016-12-20T16:58:18Z 2016-12-20T16:58:18Z Journal Article https://hdl.handle.net/10568/78422 en Limited Access Diva Enterprises Private Limited Pandian, C.S., Reddy, T.K., Sivaiah, K., Blümmel, M. and Ramana Reddy, Y. 2016. Faecal matter as inoculum for in vitro gas production. Animal Nutrition and Feed Technology 16(2): 271-281.
spellingShingle animal feeding
livestock
Pandian, C.S.
Reddy, T.K.
Sivaiah
Blümmel, Michael
Ramana Reddy, Y.
Faecal matter as inoculum for in vitro gas production
title Faecal matter as inoculum for in vitro gas production
title_full Faecal matter as inoculum for in vitro gas production
title_fullStr Faecal matter as inoculum for in vitro gas production
title_full_unstemmed Faecal matter as inoculum for in vitro gas production
title_short Faecal matter as inoculum for in vitro gas production
title_sort faecal matter as inoculum for in vitro gas production
topic animal feeding
livestock
url https://hdl.handle.net/10568/78422
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