The Bonsai as an alternative safety duplication system of the world cassava collection preserved at CIAT
The germplasm bank of CIAT houses more than 6,000 accessions of cassava (Manihot esculenta Crantz) constituting the most important collection for this crop. In order to guarantee the safety of the materials, the collection has an international duplicate kept at the International Potato Center (CIP)...
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| Format: | Poster |
| Language: | Inglés |
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International Center for Tropical Agriculture
2016
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/78102 |
| _version_ | 1855513968931504128 |
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| author | Niño, Diana Patricia Aranzales Rondón, Ericson Erazo, Juan Camilo López, Jhon Jenry Vélez, Mónica |
| author_browse | Aranzales Rondón, Ericson Erazo, Juan Camilo López, Jhon Jenry Niño, Diana Patricia Vélez, Mónica |
| author_facet | Niño, Diana Patricia Aranzales Rondón, Ericson Erazo, Juan Camilo López, Jhon Jenry Vélez, Mónica |
| author_sort | Niño, Diana Patricia |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The germplasm bank of CIAT houses more than 6,000 accessions of cassava (Manihot esculenta Crantz) constituting the most important collection for this crop. In order to guarantee the safety of the materials, the collection has an international duplicate kept at the International Potato Center (CIP) in Peru under in vitro conditions. However, due to the difficulties in having this system of duplication sustained for long periods of time, it was decided to keep the backup on a slow-growth scheme under greenhouse conditions. In vitro plants must undergo a hardening or acclimatization phase of approximately four weeks, which takes into account factors such as substrate, control of environmental conditions and pathogens to prevent loss of material. In this work, the use of a methodology for the establishment of materials in greenhouse was evaluated and compared with four systems previously used for this purpose. Modification in the substrate, the use of a biological inoculant, moist chamber conditions and fertilization allowed the establishment of 1,818 accessions with a loss rate of less than 4%, compared to the initial number of plants (1,886) which indicates that the methodology evaluated is adequate for their hardening. The plants were obtained from the in vitro cassava collection and were tested and found free from three diseases considered of quarantine for the Americas: Cassava common mosaic virus, Cassava frogskin agent and Cassava virus “X”. These materials have also been tested and found to be free of Reovirus, Luteovirus, Torradovirus and Potexvirus. The bonsai copy allows to safeguard a greater percentage of accessions for prolonged periods, since, by restricting the growth of the root in plastic containers and with controlled prunings, it is possible to maintain the plants for more than two years, time greater than achieved with the duplication systemwith in vitro techniques. |
| format | Poster |
| id | CGSpace78102 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | International Center for Tropical Agriculture |
| publisherStr | International Center for Tropical Agriculture |
| record_format | dspace |
| spelling | CGSpace781022025-11-05T16:09:49Z The Bonsai as an alternative safety duplication system of the world cassava collection preserved at CIAT Niño, Diana Patricia Aranzales Rondón, Ericson Erazo, Juan Camilo López, Jhon Jenry Vélez, Mónica manihot esculenta crantz genetic resources in vitro conservation germplasm bonsai recursos genéticos conservación germoplasma The germplasm bank of CIAT houses more than 6,000 accessions of cassava (Manihot esculenta Crantz) constituting the most important collection for this crop. In order to guarantee the safety of the materials, the collection has an international duplicate kept at the International Potato Center (CIP) in Peru under in vitro conditions. However, due to the difficulties in having this system of duplication sustained for long periods of time, it was decided to keep the backup on a slow-growth scheme under greenhouse conditions. In vitro plants must undergo a hardening or acclimatization phase of approximately four weeks, which takes into account factors such as substrate, control of environmental conditions and pathogens to prevent loss of material. In this work, the use of a methodology for the establishment of materials in greenhouse was evaluated and compared with four systems previously used for this purpose. Modification in the substrate, the use of a biological inoculant, moist chamber conditions and fertilization allowed the establishment of 1,818 accessions with a loss rate of less than 4%, compared to the initial number of plants (1,886) which indicates that the methodology evaluated is adequate for their hardening. The plants were obtained from the in vitro cassava collection and were tested and found free from three diseases considered of quarantine for the Americas: Cassava common mosaic virus, Cassava frogskin agent and Cassava virus “X”. These materials have also been tested and found to be free of Reovirus, Luteovirus, Torradovirus and Potexvirus. The bonsai copy allows to safeguard a greater percentage of accessions for prolonged periods, since, by restricting the growth of the root in plastic containers and with controlled prunings, it is possible to maintain the plants for more than two years, time greater than achieved with the duplication systemwith in vitro techniques. 2016-11 2016-12-02T13:41:48Z 2016-12-02T13:41:48Z Poster https://hdl.handle.net/10568/78102 en Open Access application/pdf International Center for Tropical Agriculture Niño, Diana Patricia; Aranzales Rondón, Ericson; Erazo, Juan Camilo; López, Jhon Jenry; Vélez, Monica. 2016. The Bonsai as an alternative safety duplication system of the world cassava collection preserved at CIAT . Centro Internacional de Agricultura Tropical (CIAT), Cali. CO. 1 p. (Poster presentado en el II Simposio de Recursos Fitogenéticos Neotropicales, 3 al 5 de Noviembre del 2.016. Universidad Nacional de Colombia sede Palmira. Valle del Cauca. Colombia). |
| spellingShingle | manihot esculenta crantz genetic resources in vitro conservation germplasm bonsai recursos genéticos conservación germoplasma Niño, Diana Patricia Aranzales Rondón, Ericson Erazo, Juan Camilo López, Jhon Jenry Vélez, Mónica The Bonsai as an alternative safety duplication system of the world cassava collection preserved at CIAT |
| title | The Bonsai as an alternative safety duplication system of the world cassava collection preserved at CIAT |
| title_full | The Bonsai as an alternative safety duplication system of the world cassava collection preserved at CIAT |
| title_fullStr | The Bonsai as an alternative safety duplication system of the world cassava collection preserved at CIAT |
| title_full_unstemmed | The Bonsai as an alternative safety duplication system of the world cassava collection preserved at CIAT |
| title_short | The Bonsai as an alternative safety duplication system of the world cassava collection preserved at CIAT |
| title_sort | bonsai as an alternative safety duplication system of the world cassava collection preserved at ciat |
| topic | manihot esculenta crantz genetic resources in vitro conservation germplasm bonsai recursos genéticos conservación germoplasma |
| url | https://hdl.handle.net/10568/78102 |
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