Pathogenic and molecular characterisation of Pythium spp. inducing root rot symptoms in other crops intercropped with beans in Southwestern Uganda
Objective: In Southwestern Uganda, bean root rot epidemics associated with Pythium species are frequent despite the use of various management methods. This study set out to determine whether other crops in bean cropping systems of Southwestern Uganda are affected by Pythium root rots and to characte...
| Autores principales: | , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
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African Journals Online
2016
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| Acceso en línea: | https://hdl.handle.net/10568/77862 |
| _version_ | 1855526296449187840 |
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| author | Gichuru, Virginia Buruchara, Robin Arani Okori, Patrick |
| author_browse | Buruchara, Robin Arani Gichuru, Virginia Okori, Patrick |
| author_facet | Gichuru, Virginia Buruchara, Robin Arani Okori, Patrick |
| author_sort | Gichuru, Virginia |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Objective: In Southwestern Uganda, bean root rot epidemics associated with Pythium species are frequent despite the use of various management methods. This study set out to determine whether other crops in bean cropping systems of Southwestern Uganda are affected by Pythium root rots and to characterise the Pythium species using Internal transcribed sequence (ITS) DNA primers.
Methodology and Results: Root rots were found to occur on maize, sorghum, peas and potato sampled from farmer’s fields where they were found to be intercropped with beans affected by root rot. Pythium species were isolated using Corn meal agar (CMA).DNA was subsequently extracted and polymerase chain reaction ( PCR) analysis carried out using ITS DNA region primers and then the PCR products were sequenced. Twenty-one Pythium species were isolated. Cross pathogenicity tests were done in a screen house using bean pathogenic Pythium species and Pythium, species derived from other crops intercropped with beans. The Pythium species were moderately to non-pathogenic in maize and millet while those in sorghum and peas were highly pathogenic.
Conclusion and application of results: This study found that other crops intercropped with beans in Southwestern Uganda were affected by Pythium root rots. Peas and sorghum were highly susceptible to the pathogenic Pythium species and could therefore be contributing to the continuous bean root rot epiphytotics in the region. On the other hand, maize and millet were non pathogenic to the Pythium species. This knowledge could be used to advice farmers not to include sorghum and peas when intercropping with beans, as they seem to be contributing to the Pythium inoculum load. They could alternatively use maize and millet, as they are not susceptible to the pathogenic Pythium species. |
| format | Journal Article |
| id | CGSpace77862 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | African Journals Online |
| publisherStr | African Journals Online |
| record_format | dspace |
| spelling | CGSpace778622025-03-13T09:44:19Z Pathogenic and molecular characterisation of Pythium spp. inducing root rot symptoms in other crops intercropped with beans in Southwestern Uganda Gichuru, Virginia Buruchara, Robin Arani Okori, Patrick phaseolus vulgaris pythium root rots pathology beans pest control dna podredumbre de la raíz control de plagas Objective: In Southwestern Uganda, bean root rot epidemics associated with Pythium species are frequent despite the use of various management methods. This study set out to determine whether other crops in bean cropping systems of Southwestern Uganda are affected by Pythium root rots and to characterise the Pythium species using Internal transcribed sequence (ITS) DNA primers. Methodology and Results: Root rots were found to occur on maize, sorghum, peas and potato sampled from farmer’s fields where they were found to be intercropped with beans affected by root rot. Pythium species were isolated using Corn meal agar (CMA).DNA was subsequently extracted and polymerase chain reaction ( PCR) analysis carried out using ITS DNA region primers and then the PCR products were sequenced. Twenty-one Pythium species were isolated. Cross pathogenicity tests were done in a screen house using bean pathogenic Pythium species and Pythium, species derived from other crops intercropped with beans. The Pythium species were moderately to non-pathogenic in maize and millet while those in sorghum and peas were highly pathogenic. Conclusion and application of results: This study found that other crops intercropped with beans in Southwestern Uganda were affected by Pythium root rots. Peas and sorghum were highly susceptible to the pathogenic Pythium species and could therefore be contributing to the continuous bean root rot epiphytotics in the region. On the other hand, maize and millet were non pathogenic to the Pythium species. This knowledge could be used to advice farmers not to include sorghum and peas when intercropping with beans, as they seem to be contributing to the Pythium inoculum load. They could alternatively use maize and millet, as they are not susceptible to the pathogenic Pythium species. 2016 2016-11-24T20:51:07Z 2016-11-24T20:51:07Z Journal Article https://hdl.handle.net/10568/77862 en Open Access African Journals Online Gichuru, Virginia; Buruchara, Robin; Okori, Patrick. 2016. Pathogenic and molecular characterisation of Pythium spp. inducing root rot symptoms in other crops intercropped with beans in Southwestern Uganda . Journal of Applied Biosciences 104: 9955: 9964. |
| spellingShingle | phaseolus vulgaris pythium root rots pathology beans pest control dna podredumbre de la raíz control de plagas Gichuru, Virginia Buruchara, Robin Arani Okori, Patrick Pathogenic and molecular characterisation of Pythium spp. inducing root rot symptoms in other crops intercropped with beans in Southwestern Uganda |
| title | Pathogenic and molecular characterisation of Pythium spp. inducing root rot symptoms in other crops intercropped with beans in Southwestern Uganda |
| title_full | Pathogenic and molecular characterisation of Pythium spp. inducing root rot symptoms in other crops intercropped with beans in Southwestern Uganda |
| title_fullStr | Pathogenic and molecular characterisation of Pythium spp. inducing root rot symptoms in other crops intercropped with beans in Southwestern Uganda |
| title_full_unstemmed | Pathogenic and molecular characterisation of Pythium spp. inducing root rot symptoms in other crops intercropped with beans in Southwestern Uganda |
| title_short | Pathogenic and molecular characterisation of Pythium spp. inducing root rot symptoms in other crops intercropped with beans in Southwestern Uganda |
| title_sort | pathogenic and molecular characterisation of pythium spp inducing root rot symptoms in other crops intercropped with beans in southwestern uganda |
| topic | phaseolus vulgaris pythium root rots pathology beans pest control dna podredumbre de la raíz control de plagas |
| url | https://hdl.handle.net/10568/77862 |
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