Complete sequence and variability of a new subgroup B nepovirus infecting potato in central Peru
The complete bipartite genome (RNA1 and RNA2) of a new nepovirus infecting potato was obtained using small RNA sequencing and assembly complemented by Sanger sequencing. Each RNA encodes a single polyprotein, flanked by 5’ and 3’ untranslate regions (UTR) and followed by a poly (A) tail. The putativ...
| Main Authors: | , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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Springer
2017
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| Online Access: | https://hdl.handle.net/10568/77836 |
| _version_ | 1855537291924078592 |
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| author | Souza, Joao de Muller, G. Pérez, W. Cuéllar, Wilmer Jose Kreuze, Jan F. |
| author_browse | Cuéllar, Wilmer Jose Kreuze, Jan F. Muller, G. Pérez, W. Souza, Joao de |
| author_facet | Souza, Joao de Muller, G. Pérez, W. Cuéllar, Wilmer Jose Kreuze, Jan F. |
| author_sort | Souza, Joao de |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The complete bipartite genome (RNA1 and RNA2) of a new nepovirus infecting potato was obtained using small RNA sequencing and assembly complemented by Sanger sequencing. Each RNA encodes a single polyprotein, flanked by 5’ and 3’ untranslate regions (UTR) and followed by a poly (A) tail. The putative polyproteins encoded by RNA1 and RNA2 had sets of motifs which are characteristic of viruses in the genus Nepovirus. Sequence comparisons using the Pro-Pol region and the coat protein, including phylogenetic analysis of these regions, showed closest relationships with nepoviruses. The data obtained support the taxonomical status of this new virus (putative named Potato virus B, PVB) as a member of the genus Nepovirus, subgroup B. |
| format | Journal Article |
| id | CGSpace77836 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace778362025-03-13T09:45:12Z Complete sequence and variability of a new subgroup B nepovirus infecting potato in central Peru Souza, Joao de Muller, G. Pérez, W. Cuéllar, Wilmer Jose Kreuze, Jan F. viroses nepoviruses genomes potatoes rna virosis nepovirus genomas papa arn The complete bipartite genome (RNA1 and RNA2) of a new nepovirus infecting potato was obtained using small RNA sequencing and assembly complemented by Sanger sequencing. Each RNA encodes a single polyprotein, flanked by 5’ and 3’ untranslate regions (UTR) and followed by a poly (A) tail. The putative polyproteins encoded by RNA1 and RNA2 had sets of motifs which are characteristic of viruses in the genus Nepovirus. Sequence comparisons using the Pro-Pol region and the coat protein, including phylogenetic analysis of these regions, showed closest relationships with nepoviruses. The data obtained support the taxonomical status of this new virus (putative named Potato virus B, PVB) as a member of the genus Nepovirus, subgroup B. 2017-03 2016-11-22T17:33:53Z 2016-11-22T17:33:53Z Journal Article https://hdl.handle.net/10568/77836 en Open Access Springer De Souza, Joao; Müller, Giovanna; Perez, Wilmer; Cuellar, Wilmer; Kreuze, Jan. 2016. Complete sequence and variability of a new subgroup B nepovirus infecting potato in central Peru . Archives of Virology 1-5 p. |
| spellingShingle | viroses nepoviruses genomes potatoes rna virosis nepovirus genomas papa arn Souza, Joao de Muller, G. Pérez, W. Cuéllar, Wilmer Jose Kreuze, Jan F. Complete sequence and variability of a new subgroup B nepovirus infecting potato in central Peru |
| title | Complete sequence and variability of a new subgroup B nepovirus infecting potato in central Peru |
| title_full | Complete sequence and variability of a new subgroup B nepovirus infecting potato in central Peru |
| title_fullStr | Complete sequence and variability of a new subgroup B nepovirus infecting potato in central Peru |
| title_full_unstemmed | Complete sequence and variability of a new subgroup B nepovirus infecting potato in central Peru |
| title_short | Complete sequence and variability of a new subgroup B nepovirus infecting potato in central Peru |
| title_sort | complete sequence and variability of a new subgroup b nepovirus infecting potato in central peru |
| topic | viroses nepoviruses genomes potatoes rna virosis nepovirus genomas papa arn |
| url | https://hdl.handle.net/10568/77836 |
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