Differential infection patterns and recent evolutionary origins of equine hepaciviruses in donkeys
The hepatitis C virus (HCV) is a major human pathogen. Genetically related viruses in animals suggest a zoonotic origin of HCV. The closest relative of HCV is found in horses (termed equine hepacivirus, EqHV). However, low EqHV genetic diversity implies relatively recent acquisition of EqHV by horse...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Journal Article |
| Lenguaje: | Inglés |
| Publicado: |
American Society for Microbiology
2017
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/10568/77699 |
| _version_ | 1855541253404360704 |
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| author | Walter, S. Rasche, A. Moreira-Soto, A. Pfaender, S. Bletsa, M. Corman, V.M. Aguilar-Setien, A. García Lacy, F. Hans, A. Todt, D. Schuler, G. Shnaiderman-Torban, A. Steinman, A. Roncoroni, C. Venezian, V. Rusenova, N. Sandev, N. Rusenov, A. Zapryanova, D. García Bocanegra, I. Jores, Joerg Carluccio, A. Veronesi, M.C. Cavalleri, J.M.V. Drosten, C. Lemey, P. Steinmann, E. Drexler, J.F. |
| author_browse | Aguilar-Setien, A. Bletsa, M. Carluccio, A. Cavalleri, J.M.V. Corman, V.M. Drexler, J.F. Drosten, C. García Bocanegra, I. García Lacy, F. Hans, A. Jores, Joerg Lemey, P. Moreira-Soto, A. Pfaender, S. Rasche, A. Roncoroni, C. Rusenov, A. Rusenova, N. Sandev, N. Schuler, G. Shnaiderman-Torban, A. Steinman, A. Steinmann, E. Todt, D. Venezian, V. Veronesi, M.C. Walter, S. Zapryanova, D. |
| author_facet | Walter, S. Rasche, A. Moreira-Soto, A. Pfaender, S. Bletsa, M. Corman, V.M. Aguilar-Setien, A. García Lacy, F. Hans, A. Todt, D. Schuler, G. Shnaiderman-Torban, A. Steinman, A. Roncoroni, C. Venezian, V. Rusenova, N. Sandev, N. Rusenov, A. Zapryanova, D. García Bocanegra, I. Jores, Joerg Carluccio, A. Veronesi, M.C. Cavalleri, J.M.V. Drosten, C. Lemey, P. Steinmann, E. Drexler, J.F. |
| author_sort | Walter, S. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The hepatitis C virus (HCV) is a major human pathogen. Genetically related viruses in animals suggest a zoonotic origin of HCV. The closest relative of HCV is found in horses (termed equine hepacivirus, EqHV). However, low EqHV genetic diversity implies relatively recent acquisition of EqHV by horses, making a derivation of HCV from EqHV unlikely. To unravel the EqHV evolutionary history within equid sister species, we analyzed 829 donkeys and 53 mules sampled in nine European, Asian, African and American countries by molecular and serologic tools for EqHV infection. Antibodies were found in 278 animals (31.5%), and viral RNA was found in 3 animals (0.3%), all of which were simultaneously seropositive. A low RNA prevalence in spite of high seroprevalence suggests predominance of acute infection, a possible difference from the mostly chronic hepacivirus infection pattern seen in horses and humans. Limitation of transmission due to short courses of infection may explain the existence of entirely seronegative groups of animals. Donkey and horse EqHV strains were paraphyletic and 97.5-98.2% identical in their translated polyprotein sequences, making virus/host co-speciation unlikely. Evolutionary reconstructions supported host switches of EqHV between horses and donkeys without the involvement of adaptive evolution. Global admixture of donkey and horse hepaciviruses was compatible with anthropogenic alterations of EqHV ecology. In summary, our findings do not support EqHV as the origin of the significantly more diversified HCV. Identification of a host system with predominantly acute hepacivirus infection may enable new insights into the chronic infection pattern associated with HCV. |
| format | Journal Article |
| id | CGSpace77699 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| publisher | American Society for Microbiology |
| publisherStr | American Society for Microbiology |
| record_format | dspace |
| spelling | CGSpace776992025-12-08T09:54:28Z Differential infection patterns and recent evolutionary origins of equine hepaciviruses in donkeys Walter, S. Rasche, A. Moreira-Soto, A. Pfaender, S. Bletsa, M. Corman, V.M. Aguilar-Setien, A. García Lacy, F. Hans, A. Todt, D. Schuler, G. Shnaiderman-Torban, A. Steinman, A. Roncoroni, C. Venezian, V. Rusenova, N. Sandev, N. Rusenov, A. Zapryanova, D. García Bocanegra, I. Jores, Joerg Carluccio, A. Veronesi, M.C. Cavalleri, J.M.V. Drosten, C. Lemey, P. Steinmann, E. Drexler, J.F. animal diseases The hepatitis C virus (HCV) is a major human pathogen. Genetically related viruses in animals suggest a zoonotic origin of HCV. The closest relative of HCV is found in horses (termed equine hepacivirus, EqHV). However, low EqHV genetic diversity implies relatively recent acquisition of EqHV by horses, making a derivation of HCV from EqHV unlikely. To unravel the EqHV evolutionary history within equid sister species, we analyzed 829 donkeys and 53 mules sampled in nine European, Asian, African and American countries by molecular and serologic tools for EqHV infection. Antibodies were found in 278 animals (31.5%), and viral RNA was found in 3 animals (0.3%), all of which were simultaneously seropositive. A low RNA prevalence in spite of high seroprevalence suggests predominance of acute infection, a possible difference from the mostly chronic hepacivirus infection pattern seen in horses and humans. Limitation of transmission due to short courses of infection may explain the existence of entirely seronegative groups of animals. Donkey and horse EqHV strains were paraphyletic and 97.5-98.2% identical in their translated polyprotein sequences, making virus/host co-speciation unlikely. Evolutionary reconstructions supported host switches of EqHV between horses and donkeys without the involvement of adaptive evolution. Global admixture of donkey and horse hepaciviruses was compatible with anthropogenic alterations of EqHV ecology. In summary, our findings do not support EqHV as the origin of the significantly more diversified HCV. Identification of a host system with predominantly acute hepacivirus infection may enable new insights into the chronic infection pattern associated with HCV. 2017-01 2016-11-10T15:05:49Z 2016-11-10T15:05:49Z Journal Article https://hdl.handle.net/10568/77699 en Open Access American Society for Microbiology Walter, S., Rasche, A., Moreira-Soto, A., Pfaender, S., Bletsa, M., Corman, V.M., Aguilar-Setien, A., García-Lacy, F., Hans, A., Todt, D., Schuler, G., Shnaiderman-Torban, A., Steinman, A., Roncoroni, C., Veneziano, V., Rusenova, N., Sandev, N., Rusenov, A., Zapryanova, D., García-Bocanegra, I., Jores, J., Carluccio, A., Veronesi, M.C., Cavalleri, J.M.V., Drosten, C., Lemey, P., Steinmann, E. and Drexler, J.F. 2017. Differential infection patterns and recent evolutionary origins of equine hepaciviruses in donkeys. Journal of Virology 91(1):e01711-16. |
| spellingShingle | animal diseases Walter, S. Rasche, A. Moreira-Soto, A. Pfaender, S. Bletsa, M. Corman, V.M. Aguilar-Setien, A. García Lacy, F. Hans, A. Todt, D. Schuler, G. Shnaiderman-Torban, A. Steinman, A. Roncoroni, C. Venezian, V. Rusenova, N. Sandev, N. Rusenov, A. Zapryanova, D. García Bocanegra, I. Jores, Joerg Carluccio, A. Veronesi, M.C. Cavalleri, J.M.V. Drosten, C. Lemey, P. Steinmann, E. Drexler, J.F. Differential infection patterns and recent evolutionary origins of equine hepaciviruses in donkeys |
| title | Differential infection patterns and recent evolutionary origins of equine hepaciviruses in donkeys |
| title_full | Differential infection patterns and recent evolutionary origins of equine hepaciviruses in donkeys |
| title_fullStr | Differential infection patterns and recent evolutionary origins of equine hepaciviruses in donkeys |
| title_full_unstemmed | Differential infection patterns and recent evolutionary origins of equine hepaciviruses in donkeys |
| title_short | Differential infection patterns and recent evolutionary origins of equine hepaciviruses in donkeys |
| title_sort | differential infection patterns and recent evolutionary origins of equine hepaciviruses in donkeys |
| topic | animal diseases |
| url | https://hdl.handle.net/10568/77699 |
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