Generation of transgenic plantain (Musa spp.) with resistance to plant pathogenic nematodes
Plant parasitic nematodes impose a severe constraint on plantain and banana productivity; however, the sterile nature of many cultivars precludes conventional breeding for resistance. Transgenic plantain cv. Gonja manjaya (Musa AAB) plants, expressing a maize cystatin that inhibits nematode digestiv...
| Main Authors: | , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Wiley
2012
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/77449 |
| _version_ | 1855531931387559936 |
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| author | Roderick, H. Tripathi, L. Babirye, A. Wang, D. Tripathi, J.N. Urwin, P.E. Atkinson, H.J. |
| author_browse | Atkinson, H.J. Babirye, A. Roderick, H. Tripathi, J.N. Tripathi, L. Urwin, P.E. Wang, D. |
| author_facet | Roderick, H. Tripathi, L. Babirye, A. Wang, D. Tripathi, J.N. Urwin, P.E. Atkinson, H.J. |
| author_sort | Roderick, H. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Plant parasitic nematodes impose a severe constraint on plantain and banana productivity; however, the sterile nature of many cultivars precludes conventional breeding for resistance. Transgenic plantain cv. Gonja manjaya (Musa AAB) plants, expressing a maize cystatin that inhibits nematode digestive cysteine proteinases and a synthetic peptide that disrupts nematode chemoreception, were assessed for their ability to resist nematode infection. Lines were generated that expressed each gene singly or both together in a stacked defence. Nematode challenge with a single species or a mixed population identified 10 lines with significant resistance. The best level of resistance achieved against the major pest species Radopholus similis was 84% ± 8% for the cystatin, 66% ± 14% for the peptide and 70% ± 6% for the dual defence. In the mixed population, trial resistance was also demonstrated to Helicotylenchus multicinctus. A fluorescently labelled form of the chemodisruptive peptide underwent retrograde transport along certain sensory dendrites of R. similis as required to disrupt chemoreception. The peptide was degraded after 30 min in simulated intestinal fluid or boiling water and after 1 h in nonsterile soil. In silico sequence analysis suggests that the peptide is not a mammalian antigen. This work establishes the mode of action of a novel nematode defence, develops the evidence for its safe and effective deployment against multiple nematode species and identifies transgenic plantain lines with a high level of resistance for a proposed field trial. |
| format | Journal Article |
| id | CGSpace77449 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2012 |
| publishDateRange | 2012 |
| publishDateSort | 2012 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | CGSpace774492023-09-30T10:57:18Z Generation of transgenic plantain (Musa spp.) with resistance to plant pathogenic nematodes Roderick, H. Tripathi, L. Babirye, A. Wang, D. Tripathi, J.N. Urwin, P.E. Atkinson, H.J. transgenic plantains resistance plant pathogens nematodes Plant parasitic nematodes impose a severe constraint on plantain and banana productivity; however, the sterile nature of many cultivars precludes conventional breeding for resistance. Transgenic plantain cv. Gonja manjaya (Musa AAB) plants, expressing a maize cystatin that inhibits nematode digestive cysteine proteinases and a synthetic peptide that disrupts nematode chemoreception, were assessed for their ability to resist nematode infection. Lines were generated that expressed each gene singly or both together in a stacked defence. Nematode challenge with a single species or a mixed population identified 10 lines with significant resistance. The best level of resistance achieved against the major pest species Radopholus similis was 84% ± 8% for the cystatin, 66% ± 14% for the peptide and 70% ± 6% for the dual defence. In the mixed population, trial resistance was also demonstrated to Helicotylenchus multicinctus. A fluorescently labelled form of the chemodisruptive peptide underwent retrograde transport along certain sensory dendrites of R. similis as required to disrupt chemoreception. The peptide was degraded after 30 min in simulated intestinal fluid or boiling water and after 1 h in nonsterile soil. In silico sequence analysis suggests that the peptide is not a mammalian antigen. This work establishes the mode of action of a novel nematode defence, develops the evidence for its safe and effective deployment against multiple nematode species and identifies transgenic plantain lines with a high level of resistance for a proposed field trial. 2012-10 2016-10-27T08:30:59Z 2016-10-27T08:30:59Z Journal Article https://hdl.handle.net/10568/77449 en Open Access Wiley Roderick, H., Tripathi, L., Babirye, A., Wang, D., Tripathi, J., Urwin, P.E. & Atkinson, H.J. (2012). Generation of transgenic plantain (Musa spp.) with resistance to plant pathogenic nematodes. Molecular Plant Pathology, 13(8), 842-851. |
| spellingShingle | transgenic plantains resistance plant pathogens nematodes Roderick, H. Tripathi, L. Babirye, A. Wang, D. Tripathi, J.N. Urwin, P.E. Atkinson, H.J. Generation of transgenic plantain (Musa spp.) with resistance to plant pathogenic nematodes |
| title | Generation of transgenic plantain (Musa spp.) with resistance to plant pathogenic nematodes |
| title_full | Generation of transgenic plantain (Musa spp.) with resistance to plant pathogenic nematodes |
| title_fullStr | Generation of transgenic plantain (Musa spp.) with resistance to plant pathogenic nematodes |
| title_full_unstemmed | Generation of transgenic plantain (Musa spp.) with resistance to plant pathogenic nematodes |
| title_short | Generation of transgenic plantain (Musa spp.) with resistance to plant pathogenic nematodes |
| title_sort | generation of transgenic plantain musa spp with resistance to plant pathogenic nematodes |
| topic | transgenic plantains resistance plant pathogens nematodes |
| url | https://hdl.handle.net/10568/77449 |
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