Genetic diversity of Colletotrichum gloeosporioides in Nigeria using amplified fragment length polymorphismA (AFLP) markers

Colletotrichum gloeosporioides is the causal agent of yam anthracnose disease in Nigeria. Differential cultivars and amplified fragment length polymorphic DNA markers were used to assess the extent of genetic diversity among 39 isolates of the pathogen. Fourteen (14) pathotypes of the pathogen were...

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Main Authors: Aduramigba-Modupe, A.O., Asiedu, Robert, Odebode, C.A., Owolade, F.
Format: Journal Article
Language:Inglés
Published: Academic Journals 2012
Subjects:
Online Access:https://hdl.handle.net/10568/77410
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author Aduramigba-Modupe, A.O.
Asiedu, Robert
Odebode, C.A.
Owolade, F.
author_browse Aduramigba-Modupe, A.O.
Asiedu, Robert
Odebode, C.A.
Owolade, F.
author_facet Aduramigba-Modupe, A.O.
Asiedu, Robert
Odebode, C.A.
Owolade, F.
author_sort Aduramigba-Modupe, A.O.
collection Repository of Agricultural Research Outputs (CGSpace)
description Colletotrichum gloeosporioides is the causal agent of yam anthracnose disease in Nigeria. Differential cultivars and amplified fragment length polymorphic DNA markers were used to assess the extent of genetic diversity among 39 isolates of the pathogen. Fourteen (14) pathotypes of the pathogen were identified based on inoculation of a differential set of Dioscorea alata genotypes of which pathotype P11 was the most virulent attacking all differential D. alata host genotypes, while pathotype P8 was the least virulent. 52% of the isolates were avirulent, 23% were slightly virulent and 25% were virulent. Amplified fragment length polymorphism (AFLP) analysis confirmed genetic variation among the C. gloeosporioides isolates and was effective in establishing genetic relationships between them. However, the grouping of the isolates based on AFLP analysis was not directly related to virulence groups or geographical origin of the isolates. The genetic variation in C. gloeosporioides is important in choosing strategies to develop durable resistance
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spelling CGSpace774102024-05-01T08:17:23Z Genetic diversity of Colletotrichum gloeosporioides in Nigeria using amplified fragment length polymorphismA (AFLP) markers Aduramigba-Modupe, A.O. Asiedu, Robert Odebode, C.A. Owolade, F. pathotypes genetic variation amplified fragment length polymorphism yams dna Colletotrichum gloeosporioides is the causal agent of yam anthracnose disease in Nigeria. Differential cultivars and amplified fragment length polymorphic DNA markers were used to assess the extent of genetic diversity among 39 isolates of the pathogen. Fourteen (14) pathotypes of the pathogen were identified based on inoculation of a differential set of Dioscorea alata genotypes of which pathotype P11 was the most virulent attacking all differential D. alata host genotypes, while pathotype P8 was the least virulent. 52% of the isolates were avirulent, 23% were slightly virulent and 25% were virulent. Amplified fragment length polymorphism (AFLP) analysis confirmed genetic variation among the C. gloeosporioides isolates and was effective in establishing genetic relationships between them. However, the grouping of the isolates based on AFLP analysis was not directly related to virulence groups or geographical origin of the isolates. The genetic variation in C. gloeosporioides is important in choosing strategies to develop durable resistance 2012-04 2016-10-27T08:30:14Z 2016-10-27T08:30:14Z Journal Article https://hdl.handle.net/10568/77410 en Limited Access Academic Journals Aduramigba-Modupe, A.O., Asiedu, R., Odebode, C.A. & Owolade, F. (2012). Genetic diversity of Colletotrichum gloeosporioides in Nigeria using amplified fragment length polymorphism (AFLP) markers. African Journal of Biotechnology, 11(33), 8189-8195.
spellingShingle pathotypes
genetic variation
amplified fragment length polymorphism
yams
dna
Aduramigba-Modupe, A.O.
Asiedu, Robert
Odebode, C.A.
Owolade, F.
Genetic diversity of Colletotrichum gloeosporioides in Nigeria using amplified fragment length polymorphismA (AFLP) markers
title Genetic diversity of Colletotrichum gloeosporioides in Nigeria using amplified fragment length polymorphismA (AFLP) markers
title_full Genetic diversity of Colletotrichum gloeosporioides in Nigeria using amplified fragment length polymorphismA (AFLP) markers
title_fullStr Genetic diversity of Colletotrichum gloeosporioides in Nigeria using amplified fragment length polymorphismA (AFLP) markers
title_full_unstemmed Genetic diversity of Colletotrichum gloeosporioides in Nigeria using amplified fragment length polymorphismA (AFLP) markers
title_short Genetic diversity of Colletotrichum gloeosporioides in Nigeria using amplified fragment length polymorphismA (AFLP) markers
title_sort genetic diversity of colletotrichum gloeosporioides in nigeria using amplified fragment length polymorphisma aflp markers
topic pathotypes
genetic variation
amplified fragment length polymorphism
yams
dna
url https://hdl.handle.net/10568/77410
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