How do roots respond to osmotic stress? A transcriptomic approach to address this question in a non-model crop
Drought is a complex phenomenon that is relevant for many crops. Performing high-throughput transcriptomics in non-model crops is challenging. The non-model crop where our workflow has been tested on is banana (Musa spp.), which ranks among the top ten staple foods (total production over 145 million...
| Main Authors: | , , , , , , |
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| Format: | Poster |
| Language: | Inglés |
| Published: |
2016
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/77311 |
| _version_ | 1855531679483953152 |
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| author | Zorrilla-Fontanesi, Yasmín Rouard, M. Cenci, A. Kissel, E. Roux, N. Swennen, Rony L. Carpentier, Sebastien C. |
| author_browse | Carpentier, Sebastien C. Cenci, A. Kissel, E. Rouard, M. Roux, N. Swennen, Rony L. Zorrilla-Fontanesi, Yasmín |
| author_facet | Zorrilla-Fontanesi, Yasmín Rouard, M. Cenci, A. Kissel, E. Roux, N. Swennen, Rony L. Carpentier, Sebastien C. |
| author_sort | Zorrilla-Fontanesi, Yasmín |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Drought is a complex phenomenon that is relevant for many crops. Performing high-throughput transcriptomics in non-model crops is challenging. The non-model crop where our workflow has been tested on is banana (Musa spp.), which ranks among the top ten staple foods (total production over 145 million tons in 2013 (FAOstat)[1]). Bananas need vast amounts of water and even mild-drought conditions are responsible for considerable yield losses[2]. To characterize drought in the roots of different banana genotypes, we designed a lab model based on osmotic stress (5% PEG treatment for 3 days) and performed mRNA-seq analysis[3]. Using Illumina technology, 18 cDNA libraries were sequenced producing around 568 million high quality reads, of which 70-84% were mapped to the diploid reference genome[4]. We show that the applied stress leads to a drop in energy levels inducing a metabolic shift towards (i) higher oxidative respiration, (ii) alternative respiration and (iii) fermentation. We also analyzed the expression patterns of paralogous genes belonging to the same gene families and detected possible cases of sub-functionalization. |
| format | Poster |
| id | CGSpace77311 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| record_format | dspace |
| spelling | CGSpace773112025-11-05T07:16:36Z How do roots respond to osmotic stress? A transcriptomic approach to address this question in a non-model crop Zorrilla-Fontanesi, Yasmín Rouard, M. Cenci, A. Kissel, E. Roux, N. Swennen, Rony L. Carpentier, Sebastien C. drought climate change roots bananas musa osmotic stress Drought is a complex phenomenon that is relevant for many crops. Performing high-throughput transcriptomics in non-model crops is challenging. The non-model crop where our workflow has been tested on is banana (Musa spp.), which ranks among the top ten staple foods (total production over 145 million tons in 2013 (FAOstat)[1]). Bananas need vast amounts of water and even mild-drought conditions are responsible for considerable yield losses[2]. To characterize drought in the roots of different banana genotypes, we designed a lab model based on osmotic stress (5% PEG treatment for 3 days) and performed mRNA-seq analysis[3]. Using Illumina technology, 18 cDNA libraries were sequenced producing around 568 million high quality reads, of which 70-84% were mapped to the diploid reference genome[4]. We show that the applied stress leads to a drop in energy levels inducing a metabolic shift towards (i) higher oxidative respiration, (ii) alternative respiration and (iii) fermentation. We also analyzed the expression patterns of paralogous genes belonging to the same gene families and detected possible cases of sub-functionalization. 2016 2016-10-14T12:47:27Z 2016-10-14T12:47:27Z Poster https://hdl.handle.net/10568/77311 en Open Access application/pdf Zorrilla-Fontanesi, Y.; Rouard, M.; Cenci, A.; Kissel, E.; Roux, N.; Swennen, R.; Carpentier, S.C. (2016) How do roots respond to osmotic stress? A transcriptomic approach to address this question in a non-model crop. Poster presented at the Plant Biology Europe EPSO/FESB 2016 Congress, Prague, Czech Republic, June 26-30, 2016. 1 p. |
| spellingShingle | drought climate change roots bananas musa osmotic stress Zorrilla-Fontanesi, Yasmín Rouard, M. Cenci, A. Kissel, E. Roux, N. Swennen, Rony L. Carpentier, Sebastien C. How do roots respond to osmotic stress? A transcriptomic approach to address this question in a non-model crop |
| title | How do roots respond to osmotic stress? A transcriptomic approach to address this question in a non-model crop |
| title_full | How do roots respond to osmotic stress? A transcriptomic approach to address this question in a non-model crop |
| title_fullStr | How do roots respond to osmotic stress? A transcriptomic approach to address this question in a non-model crop |
| title_full_unstemmed | How do roots respond to osmotic stress? A transcriptomic approach to address this question in a non-model crop |
| title_short | How do roots respond to osmotic stress? A transcriptomic approach to address this question in a non-model crop |
| title_sort | how do roots respond to osmotic stress a transcriptomic approach to address this question in a non model crop |
| topic | drought climate change roots bananas musa osmotic stress |
| url | https://hdl.handle.net/10568/77311 |
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