Risk factors for rotavirus infection in pigs in Busia and Teso subcounties, Western Kenya

We analysed data that were previously collected for molecular characterisation of rotavirus (RV) groups A and C in pigs from Teso and Busia subcounties in Kenya to determine risk factors for its infection. The data included records from 239 randomly selected piglets aged between 1 and 6 months raise...

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Autores principales: Amimo, Joshua O., Otieno, T.F., Okoth, Edward A., Onono, J.O., Bett, Bernard K.
Formato: Journal Article
Lenguaje:Inglés
Publicado: Springer 2017
Materias:
Acceso en línea:https://hdl.handle.net/10568/77268
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author Amimo, Joshua O.
Otieno, T.F.
Okoth, Edward A.
Onono, J.O.
Bett, Bernard K.
author_browse Amimo, Joshua O.
Bett, Bernard K.
Okoth, Edward A.
Onono, J.O.
Otieno, T.F.
author_facet Amimo, Joshua O.
Otieno, T.F.
Okoth, Edward A.
Onono, J.O.
Bett, Bernard K.
author_sort Amimo, Joshua O.
collection Repository of Agricultural Research Outputs (CGSpace)
description We analysed data that were previously collected for molecular characterisation of rotavirus (RV) groups A and C in pigs from Teso and Busia subcounties in Kenya to determine risk factors for its infection. The data included records from 239 randomly selected piglets aged between 1 and 6 months raised in free range and backyard production systems. RV infection was confirmed by screening of fresh faecal samples by using reverse transcription polymerase chain reaction (RT-PCR); selected positive samples were subsequently sequenced and used for phylogenetic analysis. In this analysis, RV infection status was used as outcome variable, while the metadata collected at the time of sampling were used as predictors. A Bayesian hierarchical model which used integrated nested Laplace approximation (INLA) method was then fitted to the data. The model accounted for the spatial effect by using stochastic partial differential equations (SPDEs). Of the 239 samples screened, 206 were available for the analysis. Descriptive analyses showed that 27.7 % (57/206) of the samples were positive for rotaviruses groups A and C, 18.5 % were positive for group A rotaviruses, 5.3 % were positive for group C rotaviruses, while 3.9 % had co-infections from both groups of rotaviruses. The spatial effect was insignificant, and a simple (non-spatial) model showed that piglets (≤4 months) and those pigs kept in free range systems had higher risk of exposure to rotavirus infection as compared to older pigs (>4 months) and those tethered or housed, respectively. Intervention measures that will target these high-risk groups of pigs will be beneficial to farmers.
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spelling CGSpace772682023-12-08T19:36:04Z Risk factors for rotavirus infection in pigs in Busia and Teso subcounties, Western Kenya Amimo, Joshua O. Otieno, T.F. Okoth, Edward A. Onono, J.O. Bett, Bernard K. animal diseases swine We analysed data that were previously collected for molecular characterisation of rotavirus (RV) groups A and C in pigs from Teso and Busia subcounties in Kenya to determine risk factors for its infection. The data included records from 239 randomly selected piglets aged between 1 and 6 months raised in free range and backyard production systems. RV infection was confirmed by screening of fresh faecal samples by using reverse transcription polymerase chain reaction (RT-PCR); selected positive samples were subsequently sequenced and used for phylogenetic analysis. In this analysis, RV infection status was used as outcome variable, while the metadata collected at the time of sampling were used as predictors. A Bayesian hierarchical model which used integrated nested Laplace approximation (INLA) method was then fitted to the data. The model accounted for the spatial effect by using stochastic partial differential equations (SPDEs). Of the 239 samples screened, 206 were available for the analysis. Descriptive analyses showed that 27.7 % (57/206) of the samples were positive for rotaviruses groups A and C, 18.5 % were positive for group A rotaviruses, 5.3 % were positive for group C rotaviruses, while 3.9 % had co-infections from both groups of rotaviruses. The spatial effect was insignificant, and a simple (non-spatial) model showed that piglets (≤4 months) and those pigs kept in free range systems had higher risk of exposure to rotavirus infection as compared to older pigs (>4 months) and those tethered or housed, respectively. Intervention measures that will target these high-risk groups of pigs will be beneficial to farmers. 2017-01 2016-10-13T07:09:26Z 2016-10-13T07:09:26Z Journal Article https://hdl.handle.net/10568/77268 en Limited Access Springer Amimo, J.O., Otieno, T.F., Okoth, E., Onono, J.O. and Bett, B. 2017. Risk factors for rotavirus infection in pigs in Busia and Teso subcounties, Western Kenya. Tropical Animal Health and Production 49(1): 105–112.
spellingShingle animal diseases
swine
Amimo, Joshua O.
Otieno, T.F.
Okoth, Edward A.
Onono, J.O.
Bett, Bernard K.
Risk factors for rotavirus infection in pigs in Busia and Teso subcounties, Western Kenya
title Risk factors for rotavirus infection in pigs in Busia and Teso subcounties, Western Kenya
title_full Risk factors for rotavirus infection in pigs in Busia and Teso subcounties, Western Kenya
title_fullStr Risk factors for rotavirus infection in pigs in Busia and Teso subcounties, Western Kenya
title_full_unstemmed Risk factors for rotavirus infection in pigs in Busia and Teso subcounties, Western Kenya
title_short Risk factors for rotavirus infection in pigs in Busia and Teso subcounties, Western Kenya
title_sort risk factors for rotavirus infection in pigs in busia and teso subcounties western kenya
topic animal diseases
swine
url https://hdl.handle.net/10568/77268
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