Characterization of brown streak virus–resistant cassava
Cassava brown streak disease (CBSD) has become a major constraint to cassava production in East and Central Africa. The identification of new sources of CBSD resistance is essential to deploy CBSD mitigation strategies, as the disease is progressing westwards to new geographical areas. A stringent i...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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Scientific Societies
2016
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/77146 |
| _version_ | 1855517099308351488 |
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| author | Anjanappa, R.B. Mehta, D. Maruthi, M.N. Kanju, E. Gruissem, W. Vanderschuren, H. |
| author_browse | Anjanappa, R.B. Gruissem, W. Kanju, E. Maruthi, M.N. Mehta, D. Vanderschuren, H. |
| author_facet | Anjanappa, R.B. Mehta, D. Maruthi, M.N. Kanju, E. Gruissem, W. Vanderschuren, H. |
| author_sort | Anjanappa, R.B. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Cassava brown streak disease (CBSD) has become a major constraint to cassava production in East and Central Africa. The identification of new sources of CBSD resistance is essential to deploy CBSD mitigation strategies, as the disease is progressing westwards to new geographical areas. A stringent infection method based on top cleft–grafting combined with precise virus titer quantitation was utilized to screen 14 cassava cultivars and elite breeding lines. When inoculated with mixed infections of Cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV), the scions of elite breeding lines KBH 2006/18 and KBH 2006/26 remained symptom-free during a 16-week period of virus graft inoculation, while susceptible varieties displayed typical CBSD infection symptoms at 4 weeks after grafting. The identified CBSD resistance was stable under the coinoculation of CBSV and UCBSV with cassava geminiviruses. Double-grafting experiments revealed that transmission of CBSV and UCBSV to CBSD-susceptible top scions was delayed when using intermediate scions of elite breeding lines KBH 2006/18 and KBH 2006/26. Nonetheless, comparison of virus systemic movement using scions from KBH2006/18 and a transgenic CBSD resistant 60444 line (60444-Hp9 line) showed that both CBSV and UCBSV move at undetectable levels through the stems. Further, protoplast-based assays of virus titers showed that the replication of CBSV is inhibited in the resistant line KBH2006/18, suggesting that the identified CBSD resistance is at least partially based on inhibition of virus replication. Our molecular characterization of CBSD resistance in cassava offers a robust virus-host system to further investigate the molecular determinants of CBSD resistance. |
| format | Journal Article |
| id | CGSpace77146 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | Scientific Societies |
| publisherStr | Scientific Societies |
| record_format | dspace |
| spelling | CGSpace771462024-05-01T08:15:28Z Characterization of brown streak virus–resistant cassava Anjanappa, R.B. Mehta, D. Maruthi, M.N. Kanju, E. Gruissem, W. Vanderschuren, H. cassava plant diseases physiology Cassava brown streak disease (CBSD) has become a major constraint to cassava production in East and Central Africa. The identification of new sources of CBSD resistance is essential to deploy CBSD mitigation strategies, as the disease is progressing westwards to new geographical areas. A stringent infection method based on top cleft–grafting combined with precise virus titer quantitation was utilized to screen 14 cassava cultivars and elite breeding lines. When inoculated with mixed infections of Cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV), the scions of elite breeding lines KBH 2006/18 and KBH 2006/26 remained symptom-free during a 16-week period of virus graft inoculation, while susceptible varieties displayed typical CBSD infection symptoms at 4 weeks after grafting. The identified CBSD resistance was stable under the coinoculation of CBSV and UCBSV with cassava geminiviruses. Double-grafting experiments revealed that transmission of CBSV and UCBSV to CBSD-susceptible top scions was delayed when using intermediate scions of elite breeding lines KBH 2006/18 and KBH 2006/26. Nonetheless, comparison of virus systemic movement using scions from KBH2006/18 and a transgenic CBSD resistant 60444 line (60444-Hp9 line) showed that both CBSV and UCBSV move at undetectable levels through the stems. Further, protoplast-based assays of virus titers showed that the replication of CBSV is inhibited in the resistant line KBH2006/18, suggesting that the identified CBSD resistance is at least partially based on inhibition of virus replication. Our molecular characterization of CBSD resistance in cassava offers a robust virus-host system to further investigate the molecular determinants of CBSD resistance. 2016-07 2016-09-28T09:20:30Z 2016-09-28T09:20:30Z Journal Article https://hdl.handle.net/10568/77146 en Open Access Scientific Societies Anjanappa, R.B., Mehta, D., Maruthi, M.N., Kanju, E., Gruissem, W. & Vanderschuren, H. (2016). Characterization of brown streak virus–resistant cassava. Molecular Plant-Microbe Interactions, 29(7), 527-534 |
| spellingShingle | cassava plant diseases physiology Anjanappa, R.B. Mehta, D. Maruthi, M.N. Kanju, E. Gruissem, W. Vanderschuren, H. Characterization of brown streak virus–resistant cassava |
| title | Characterization of brown streak virus–resistant cassava |
| title_full | Characterization of brown streak virus–resistant cassava |
| title_fullStr | Characterization of brown streak virus–resistant cassava |
| title_full_unstemmed | Characterization of brown streak virus–resistant cassava |
| title_short | Characterization of brown streak virus–resistant cassava |
| title_sort | characterization of brown streak virus resistant cassava |
| topic | cassava plant diseases physiology |
| url | https://hdl.handle.net/10568/77146 |
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