Survey, molecular detection and characterization of geminiviruses associated with cassava mosaic disease in Zambia
A survey was conducted from April to May 2014 in 214 farmers’ fields located across six major cassava-producing provinces (Western, Northwestern, Northern, Luapula, Lusaka, and Eastern) of Zambia to determine the status of cassava mosaic disease (CMD) and the species diversity of associated cassava...
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| Format: | Journal Article |
| Language: | Inglés |
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Scientific Societies
2016
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| Online Access: | https://hdl.handle.net/10568/77106 |
| _version_ | 1855542744554930176 |
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| author | Mulenga, R.M. Legg, James P. Ndunguru, J. Miano, D.W. Mutitu, E.W. Chikoti, Patrick Chiza Alabi, O.J. |
| author_browse | Alabi, O.J. Chikoti, Patrick Chiza Legg, James P. Miano, D.W. Mulenga, R.M. Mutitu, E.W. Ndunguru, J. |
| author_facet | Mulenga, R.M. Legg, James P. Ndunguru, J. Miano, D.W. Mutitu, E.W. Chikoti, Patrick Chiza Alabi, O.J. |
| author_sort | Mulenga, R.M. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | A survey was conducted from April to May 2014 in 214 farmers’ fields located across six major cassava-producing provinces (Western, Northwestern, Northern, Luapula, Lusaka, and Eastern) of Zambia to determine the status of cassava mosaic disease (CMD) and the species diversity of associated cassava mosaic geminiviruses (CMG). Mean CMD incidence varied across all six provinces but was greatest in Lusaka Province (81%) and least in Northern Province (44%). Mean CMD severity varied slightly between provinces, ranging from 2.78 in Eastern Province to 3.00 in Northwestern Province. Polymerase chain reaction discrimination of 226 survey samples, coupled with complete DNA-A genome sequence analysis, revealed the presence of African cassava mosaic virus (ACMV), East African cassava mosaic virus (EACMV), and East African cassava mosaic Malawi virus (EACMMV) as single or mixed infections of different proportions. Single-virus infections were predominant, occurring in 62.8% (ACMV), 5.8% (EACMMV), and 2.2% (EACMV) of samples relative to mixed-virus infections, which occurred in 19.5% (ACMV + EACMMV), 0.4% (ACMV + EACMV), and 0.9% (ACMV + EACMV + EACMMV) of samples. Phylogenetic analysis revealed the segregation of virus isolates from Zambia into clades specific to ACMV, EACMV, and EACMMV, further confirming the presence of all three viruses in Zambia. The results point to a greater diversity of CMG across major cassava-growing provinces of Zambia and implicate contaminated cassava cuttings in disease spread. |
| format | Journal Article |
| id | CGSpace77106 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | Scientific Societies |
| publisherStr | Scientific Societies |
| record_format | dspace |
| spelling | CGSpace771062024-04-25T06:01:58Z Survey, molecular detection and characterization of geminiviruses associated with cassava mosaic disease in Zambia Mulenga, R.M. Legg, James P. Ndunguru, J. Miano, D.W. Mutitu, E.W. Chikoti, Patrick Chiza Alabi, O.J. geminiviruses epidemiology genetic variation cassava A survey was conducted from April to May 2014 in 214 farmers’ fields located across six major cassava-producing provinces (Western, Northwestern, Northern, Luapula, Lusaka, and Eastern) of Zambia to determine the status of cassava mosaic disease (CMD) and the species diversity of associated cassava mosaic geminiviruses (CMG). Mean CMD incidence varied across all six provinces but was greatest in Lusaka Province (81%) and least in Northern Province (44%). Mean CMD severity varied slightly between provinces, ranging from 2.78 in Eastern Province to 3.00 in Northwestern Province. Polymerase chain reaction discrimination of 226 survey samples, coupled with complete DNA-A genome sequence analysis, revealed the presence of African cassava mosaic virus (ACMV), East African cassava mosaic virus (EACMV), and East African cassava mosaic Malawi virus (EACMMV) as single or mixed infections of different proportions. Single-virus infections were predominant, occurring in 62.8% (ACMV), 5.8% (EACMMV), and 2.2% (EACMV) of samples relative to mixed-virus infections, which occurred in 19.5% (ACMV + EACMMV), 0.4% (ACMV + EACMV), and 0.9% (ACMV + EACMV + EACMMV) of samples. Phylogenetic analysis revealed the segregation of virus isolates from Zambia into clades specific to ACMV, EACMV, and EACMMV, further confirming the presence of all three viruses in Zambia. The results point to a greater diversity of CMG across major cassava-growing provinces of Zambia and implicate contaminated cassava cuttings in disease spread. 2016-07 2016-09-20T15:21:24Z 2016-09-20T15:21:24Z Journal Article https://hdl.handle.net/10568/77106 en Open Access Scientific Societies Mulenga, R.M., Legg, J., Ndunguru, J., Miano, D.W., Mutitu, E.W., Chikoti, Patrick Chiza & Alabi, O.J. (2016). Survey, molecular detection and characterization of geminiviruses associated with cassava mosaic disease in Zambia. Plant Disease, 100(7), 1379-1387. |
| spellingShingle | geminiviruses epidemiology genetic variation cassava Mulenga, R.M. Legg, James P. Ndunguru, J. Miano, D.W. Mutitu, E.W. Chikoti, Patrick Chiza Alabi, O.J. Survey, molecular detection and characterization of geminiviruses associated with cassava mosaic disease in Zambia |
| title | Survey, molecular detection and characterization of geminiviruses associated with cassava mosaic disease in Zambia |
| title_full | Survey, molecular detection and characterization of geminiviruses associated with cassava mosaic disease in Zambia |
| title_fullStr | Survey, molecular detection and characterization of geminiviruses associated with cassava mosaic disease in Zambia |
| title_full_unstemmed | Survey, molecular detection and characterization of geminiviruses associated with cassava mosaic disease in Zambia |
| title_short | Survey, molecular detection and characterization of geminiviruses associated with cassava mosaic disease in Zambia |
| title_sort | survey molecular detection and characterization of geminiviruses associated with cassava mosaic disease in zambia |
| topic | geminiviruses epidemiology genetic variation cassava |
| url | https://hdl.handle.net/10568/77106 |
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